1988 Fiscal Year Final Research Report Summary
Renal growth factor, a novel factor purified from pituitaries: The relationship between biological activity and structure.
| Project/Area Number |
62570522
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
内分泌・代謝学
|
|---|
| Research Institution | Tokyo Women's Medical College |
|---|
Principal Investigator |
NOMURA Kaoru Tokyo Women's Medical College, 内科2, 講師 (40096623)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HORIBA Nobuo Tokyo Women's Medical College (80173649)
|
|---|
| Project Period (FY) |
1987 – 1988
|
| Keywords | Renal growth factor / Luteinizing hormone / Kidney / 糖蛋白質 |
|---|
| Research Abstract |
We demonstrated that renal growth factor(RGF) was a particular isoform of luteinizing hormone (LH) with microheterogeneity. RGF/IL was purified from porcine pituitary glands, and finally by isoelectric focusing electrophoresis (IEF). IEF separated porcine LH into four fractions based on different pI's. Only IFF-fr.2,pI ranged between 10.28 and 10,18, showed ex vivo renotropic activity. Each IEF fraction showed identical elution profile at reverse-phase HPLC, suggesting that charge heterogeneity among IEF-fractions was due to differences in carbohydrate portion, but not to those in polypeptide chains.
Analysis of carbohydrate structure of each subunit, however, failed to show structure specific to IEF-fr.2. Significant in vitro renotropic activity was demonstrated in each IEF-fraction, but their potencies were weaker in fractions with lower pI.
These findings duggest that the important role of metabolic clearance rate of each IEF fraction to express in vivo renotropic activity.
|
Research Products
(10 results)
