1988 Fiscal Year Final Research Report Summary
Induction of Activated Macrophages by Intraperitoneal Injection of MMC-CH, Mitomycin C Adsorbed on Activated Carbon Particles
| Project/Area Number |
62570575
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
General surgery
|
|---|
| Research Institution | Meiji College of Oriental Medicine |
|---|
Principal Investigator |
SAKITA Masakazu Professor of Dept. Surg. of Meiji College of Oriental Medicine, 外科学教室, 教授 (40117883)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Toshiharu Assistant of Dept. Surg. of Kyoto Prefectural Univ. of Medicine, 第1外科教室, 助手 (90111327)
|
|---|
| Project Period (FY) |
1987 – 1988
|
| Keywords | peritonitis carcimatosa / Mitomycin C adsorbed on activated carbon particles / activate macrophage / 腹腔マクロファージの活性化 / 殺細胞活性 |
|---|
| Research Abstract |
In this study, we examined the effect on the immunological status of hosts and on the cytolytic activity induced in the peritoneal macrophage(PM) by intraperitoneal(IP) injection of MMC-CH, Mitomycin C adsorbed on activated carbon particles and compared it with that of the MMC solution(MMC-AQ) itself. The weight of spleen and thymus of the mice given MMC-CH was decreased lesser than those of the mice given MMC-AQ. Reactivity of spleen cells by Con A was inhibited in the spleen cells from the mice given MMC-AQ more than that from the mice given MMC-CH. Fifty per cent lethal dose was 8 mg/kg in the mice given MMC-AQ, and 16 mg/kg in the mice given MMC-CH. Single IP injection of either MMC-AQ or MMC-CH induced tumoricidal macrophage in the peritoneal cavity. The tumoricidal activity was dependent on the dose of MMC injected and on the effector-to-target ratio and was suppressed by the presence of carrageenan. A significant difference was found in the chronological change of pec between MMC-AQ and MMC-CH administration. One day after MMC-AQ IP injection, the number of PEC wasdecreased markedly, however, it was increased after MMC-CH injection and the number was ten times more than that of MMC-AQ injection. A marked difference in the chronological change of the cytolytic activity of the PM was also observed. In the case of MMC-CH injection, cytolytic activity of PM remained high longer time than that of MMC-AQ. MMC was detected in the soluble fraction of ultrasonicated PM from either MMC-AQ or MMC-CH injected mice and these soluble fraction had a tumoricidal effect on the target cells. Based on these results, it was concluded that there is some participation of intra-cellular drug within the MMC-induced PM in tumoriciadal effects, especially after MMC-CH injection.
|
