1988 Fiscal Year Final Research Report Summary

A study of phosphoprotein produced by Dunn osteosarcoma

Research Project

Project/Area Number	62570675
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Orthopaedic surgery
Research Institution	Gunma University
Principal Investigator	UDAGAWA Eiichi Gunma University, 医学部, 教授 (70009976)
Co-Investigator(Kenkyū-buntansha)	CHIGIRA Masaki Gunma University, 医学部, 講師 (70143196)
Project Period (FY)	1987 – 1988
Keywords	Phosphoproteins / Tumor markers / Osteosarcoma clone / 予後因子
Research Abstract	A phosphoprotein (MW=6,0000) was purified from conditioned medium, complete proteinfree (GM-40), of Dunn osteosarcoma. It showed alkaline phosphatase activity. From the data, it waw shown that one of the most prominent phosphoproteins secreted by osteosarcoma is alkaline phosphatase. No correlation between the prognosis of tumor bearing host, tumor doubling time (in vivo and in vitro), the incidence of pulmonary metastasis, and the level of alkaline phosphatase production. These phenomena was analysed theoretically using gene control system and the phenotypic diversity of the tumor. Under these conditions, Corelations between the prognosis of the patients and the level of tumor markers are only phenomenological, and tumor markers should be used to demonstrate the presence of the tumor elsewhere. Biologically, there is no reason to explain the relation between the two phenomena.