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1989 Fiscal Year Final Research Report Summary

Investigation on protooncogenes in gynecologic malignancies

Research Project

Project/Area Number 62570763
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research Institution佐賀医科大学

Principal Investigator

IWASAKA Tsuyoshi  Saga Medical School Department of Obstetrics and Gynecology Assistant Professor, 医学部, 講師 (60117067)

Co-Investigator(Kenkyū-buntansha) HAYASHI Yoshinobu  Saga Medical School Department of Obstetrics and Gynecology Senior Lecturer, 医学部, 助手 (90156464)
Project Period (FY) 1987 – 1989
KeywordsCervical carcinoma / HPV type 16 / HPV type 18 / Overexpression of oncogenes / c-myc / Ha-ras / hit and run / Amplification of oncogenes
Research Abstract

Oncogenic potential of human papillomavirus (HPV) which is associated with occurrence of cervical carcinoma and mechanism of oncogenesis by this virus was investigated by transformation experiments in vitro. The results were obtained as follows. 1) Complete carcinogenesis can be mediated by HPV-16 or HPV-18 DNA under collaboration with other cofactors such as HSV-2.2) It is suggested that neoplastic transformation induced by HPV-18 DNA is based on "hit and run" oncogenesis. 3) HPV-16 or HPV-18 DNA can immortalize primary diploid cells and convert them to fully tumorigenic phenotype by repeating cell passage. 4) It has been experimentally proved that the difference in transforming potential exists between HPV 6/11 and HPV 16/18.5) Amplification and overexpression of c-myc oncogene was detected in transformed cells obtained by HPV-16 transfection. While overexpression of c-myc was detected in transformed cells induced by HPV-18 DNA, but no amplification was observed.
On the other hand, de … More tection of HPV DNA and amplification or overexpression of protooncogenes was performed in cervical intraepithelial neoplasias (CIN) and invasive cervical carcinomas. The results were summarized as follows. 1) HPV DNA was detected in approximately 70% of a population with CIN by in situ hybridization. CIN II showed the highest incidence of positive HPV DNA(91%),and the positive ratio decreased in CIN III(56%). 2) Immunohistochemical study of paraffin-embedded specimens with monoclonal antibodies to oncogene products revealed that only some of cervical invasive carcinomas expressed c-myc protein, ras p21 or EGFR. 3) HPV DNA was detected in 46% of invasive cervical carcinomas by Southern blot hybridization. The percentage of patients with positive results for HPV 16/18 was 29%. However, it increased up to 58% by use of polymerase chain reaction(PCR), suggesting that there are many cervical cancer tissues in which a number of cells lack viral DNA. 4) Northern blot hybridization analysis revealed overexpression of c-myc mRNA in 30% of cervical invasive carcinomas although amplification of c-myc oncogene was detected in only one of invasive carcinomas. Less

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] T.Iwasaka,T.Yoshimura,Y.Ohkuma,et al.: "Anticellular activities of human fibroblast interferon and human recombinant interferon." Acta Cytologica. 31. 941-945 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Iwasaka,H.Tsugitomi,Y.Ohkuma,et al.: "Anticellular activities of human recombinant interferon against cervical tumor transplanted into nude mice." Acta Obstet.Gynecol.Scandr.66. 501-505 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Iwasaka,M.Yokoyama,Y.Hayashi,et al.: "Combined herpes simplex virus type 2 and human papilloma virus type 16 or 18 DNA leads to oncogenic transformation." American.Journal of Obstetrics and Gynecology. 159. 1251-1255 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Hayashi,T.Iwasaka,M.Yokoyama,et al.: "Sensitivity of in situ hybridization for detection of human papillomavirus(HPV)as studied in HPV type 16 transfected cells." Acta Obstet.Gynaecol.Jpn.41. 2011-2012 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Hachisuga,K.Fukuda,Y.Hayashi,et al.: "Immunohistochemical demonstration of histiocytes in normal ectocervical epithelium and epithelial lesions of the uterine cervix." Gynecologic Oncology. 33. 273-278 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Iwasaka,T.Hayashi,M.Yokoyama,et al.: "Interferon treatment for cervical intraepithelial neoplasia." Gynecologic Oncology. (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Iwasaka, T.Yoshimura, Y.Ohkuma, et al.: "Anticellular activities of human fibroblast interferon and human recombinant interferon gamma." Acta Cytologica. 31. 941-945 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Iwasaka, H.Tsugitomi, Y.Ohkuma, et al.: "Anticellular activities of human recombinant interferon gamma against cervical tumor transplanted into nude mice." Acta Obstet. Gynecol. Scand. 66:501-505, 1987.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Iwasaka, M.Yokoyama, Y.Hayashi, et al.: "Combined herpes simplex virus type 2 and human papilloma virus type 16 or 18 DNA leads to oncogenic transformation." American Journal of Obstet. and Gynecol. 159:1251-1255, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Hayashi, T.Iwasaka, M.Yokoyama, et al.: "Sensitivity of insitu hybridization for detection of human papillomavirus(HPV) as studied in HPV type 16 transfected cells." Acta Obstet. Gynecol. Jpn. 41:2011-2012, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Iwasaka, Y.Hayashi, M.Yokoyama, et al.: "Interferon treatment for cervical intraepithelial neoplasia." Gynecol. Oncol. 37:96-102, 1990.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-26  

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