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1989 Fiscal Year Final Research Report Summary

Study of acid production and growth of oral streptococci under low pH and strictly anaerobic conditions.

Research Project

Project/Area Number 62570826
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionTohoku University

Principal Investigator

IWAMI Yoshimichi  Tohoku University, School of Dentistry, Research Associate., 歯学部, 助手 (60005030)

Co-Investigator(Kenkyū-buntansha) YAMADA Tadashi  Tohoku University, School of Dentistry, Professor., 歯学部, 教授 (50005021)
Project Period (FY) 1987 – 1989
KeywordsStreptoccocci / Glycolysis / Anaerobic conditions / Lactate / Acetate / Continuous Culture / Formate / Fluoride
Research Abstract

1. Production of formic arid acetic acids by Streptococcus mutatis JC2 decreased and lactic acid increased when the pH of culture medium lowered from 7.0 to 5.5. even under glucose-limitation and strictly anaerobic conditions. Two mechanisms seemed to be involved in the increased production of lactic acid at extracellular pli of 5.5. Firstly, lactate dehydrogenase(LDH) had a higher activity than pyruvate formate-lyase(PFL) in the cells. Secondly, the amount of LDH was increased remarkably in comparison to that of PFL during growth at pH 5.5.
2. Anaerobic glycolysis of Streptococcus sanguis ATCC 10556 was inhibited more strongly by fluoride than aerobic glycolysis. The cells of S. sanguis had lower pliosphoenolpyruvate(PEP) potential(intracellular levels of 3-phosphoglycerate, 2-phosphoglycerate and phosphoenolpyruvate) under anaerobic conditions than aerobic conditions. Thus it was suggested that the low PEP potential accounted for the reduced transport of sugar by the PEP-dependent phosphotransport system and consequently the reduced glycolysis of the microorganism under anaerobic conditions.
3. The acid produced by streptococci during their sugar metabolism was estimated with a pH-stat and a carboxylic acid analyzer. The amount of titratable acid estimated by the alkaline titration with the pH-stat was larger than the amount of total carboxylic acids estimated with the carboxylic acid analyzer. The results indicated that streptococci produced not only carboxylic acids, such as lactic, formic and acetic acid, but also non-carboxylic acid(s).

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Yoshimichi,Iwami: "Influence of environment on glycolysis in streptococci" Kitasato Arch.of Exp.Med.61. 1-20 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩見憙道: "酸性環境におけるstreptococcus mutansJC2の増殖と酸産生" 歯科基礎医学会雑誌. 30(補冊). 118 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩見憙道: "酸性環境におけるstreptococcus mutansJC2による乳酸産生増加" 歯科基礎医学会雑誌. 31(補冊). 102 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshimichi,Iwami: "Difference in amounts between titratable acid and total carboxylic acids produced by oral streptococci during sugar metabolism." J.Dent.Res.68. 16-19 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shinji,Hata: "Biochemical mechanisms of enhanced inhibition of fluoride on the anaerobic sugar metabolism by streptococcus sanguis." J.Dent.Res.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshimichi,Iwami: "Increased lactate production by Streptococcus mutans NCIB 11723 at low pH level under strictly anaerobic conditions."

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshimichi, Iwami: "Influence of environment on glycolysis in streptococci." Kitasato Arch. Exp. Med., 61, 1-20, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshimichi, Iwami: "Difference in amounts between titratable acid and total carboxylic acids produced by oral streptococci during sugar metabolism." J. Dent. Res., 68, 16-19, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shinji, Hata: "Biochemical mechanisms of enhanced inhibition of fluoride on the anaerobic sugar metabolism by Streptococcus sanguis." J. Dent. Res.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshimichi, Iwami: "Increased lactate production by Streptococcus mutans NCIB 11723 at low pH level under strictly anaerobic conditions."

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-26  

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