1988 Fiscal Year Final Research Report Summary
Superoxide production by leukocytes from streptozotocin induced diabetic rats and naturally occurring gingivitis rats.
| Project/Area Number |
62570851
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | Osaka Dental University |
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Principal Investigator |
MORI Masakazu Osaka Dental University, 歯学部, 教授 (20066963)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIMOTO Heizo Osaka Dental University, 歯学部, 助手 (20190077)
SHINOHARA Mitsuko Osaka Dental University, 歯学部, 助手 (40067187)
OHURA Kiyoshi Osaka Dental University, 歯学部, 講師 (20131378)
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| Project Period (FY) |
1987 – 1988
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| Keywords | Diabetes mellitus / Gingivitis / Rats / Leukocytes / 活性酸素 |
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| Research Abstract |
A common oral sign of diabetes mellitus is an increased severity in periodontal disease. In this study, we examined leukocytes abnormalities in streptozotocin diabetes rats. Superoxide (O_2^-) production of polymorphonuclear leukocytes (PMNs) and macrophages (M s) were lower in streptozotocin diabetes rats than in normal rats(p<0.05-0.01). These findings suggest that impared O_2^- production might be one of the factors accounting for impared bactericidal activity of PMNs and M s in diabetics. Additionally, it was investigated the relationship between the severity of gingivitis and abnormal M chemotaxis and O_2^- productionin rats susceptible to naturally occurring gingivitis (Sus rats). Sus rats were examined using plaque index and pocket probing depth. M chemotaxis and O_2^- production in the Sus deep pocket group (over 4.0mm pocket depth) were significantly lower than that in the Sus shallow pocket group (under 3.5mm pocket depth) and normal group (p<0.05 - 0.005). In addition, a negative correlation was found between the severity of gingivitis in Sus rats and M chemotakis and O_2^- production by Opsonized zymosan (p<0.05-0.01). These results suggest that defective M chemotaxis and superoxide production are a pathogenetic factor in destructive periodontal disease in Sus rats.
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