1988 Fiscal Year Final Research Report Summary
Analysis of biological active substances by high-performance liquid chromatography with coulometric detection
| Project/Area Number |
62570959
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | Kanazawa University (1988)
Tohoku University (1987) |
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Principal Investigator |
SHIMADA Kazutake Kanazawa University, 薬学部, 教授 (90004605)
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| Project Period (FY) |
1987 – 1988
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| Keywords | High-performance liquid chromatography / Coulometric detection / Electrophore / Ferrocene / Thiol / compound / Fatty acid / Optical active carboxylic acid |
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| Research Abstract |
High-performance liquid chromatography(HPLC) with electrochemical deteclion(ECD) is a useful method for the determination of trace components in complex matrices because of its excellent selectivity and sensitivity in previous studies, we proposed novel ferrocene reagents for the precolumn labeling of hydroxy compounds and glucuronides in HPLC-ECD. As the ferrocene derivative undergoes facile oxidation and the product is in turn readily reduced, it can be detected selectively in the presence of other electroactive compounds, such as phenols and aromatic amines.
This project has dealt with the preparation and properties of derivatization reagents having ferrocene as an electrophore for the determination of fatty acids and thiol compounds by HPLC-ECD. 3-Bromoacetyl-1,1'-dimethylferrocene and N-(ferrocenyl)maleimide were most favorable reagents for derivatization of fatty acids and thiol compounds, respectively. According to these data N-(ferrocenyl)maleimide was applied to the determination of glutathione in human blood ant the coulometric detection has been proved to be highly selective ans sensitive for the determination of biological active substances.
New derivatization methods using chiral ferroene reagents have been also developed for the optical resolution of carboxylic acids by HPLC-ECD. The diastereomeric amide formed from R-(-)-1-ferrocenylethylamine and -arylpropionic acid enantiomers were efficiently resolved within 20 min by reversed-phase chromatography with satisfactory sensitivity.
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Research Products
(6 results)
