1989 Fiscal Year Final Research Report Summary
Survey of new oncogenes in human germ cell tumors and function of oncogenes in differentiation of germ cell tumors
| Project/Area Number |
62571009
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Okayama University, Faculty of Engineering (1988-1989)
National Cancer Center Research Institute and Research Center for Innovative Oncology, National Cancer Center Hospital East (1987) |
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Principal Investigator |
KANAZAWA Hiroshi Okayama University, Dept. of Biotechnology, Professor, 工学部, 教授 (50116448)
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| Co-Investigator(Kenkyū-buntansha) |
TESHIMA Shinichi National Cancer Center Institute, Pathology Div., Chief Researcher, 病理部, 主任研究員 (40150198)
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| Project Period (FY) |
1987 – 1989
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| Keywords | human germ cell tumor / transfection / oncogenes / differentiation / N-myc |
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| Research Abstract |
Since human germ cell tumor occurs for Young ages of human, this type of tumors should have unique features in terms of its oncogenesis, compared to other-types of tumors including stomach, and lung. Therefore, we tried to obtain new type of oncogenic genes from this type of tumors by transfection assay using NIH 3T3 and EL2. a rat fibroblast cell line. We have not been able to obtain new oneogenes as a result. We also analyzed structural alteration and unusual expression of several onco genes reported previously, in tumor tissues from operational preparations and in germ cell tumor cell lines established in this study. We found amplification of N-myc gene in one of the operational preparations and also observed unusual expression of the gene in all of the cell lines studied, which was not found in other tumor cell lines including HeLa cells. These results suggested that N-myc expression contributes to oncogenesis of germ cell tumors or maintainance of its tumor state. It was also found that c-myc gene expression was repressed during differentiation of a germ cell tumor line (NCC-IT) established in this study by addition of retinole acid, while N-myc expression did not change.
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[Publications] Teshima,S.,Shimosato,Y.,Hirohashi,S.,Tome,Y.,Hayashi,I.,Kanazawa,H.,and Kakizoe,T.: "Four new human germ cell tumor cell lines" Laboratory Investigation. vol.59,. 328-336 (1988)
Description
「研究成果報告書概要(和文)」より
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[Publications] Orita,M.,Iwahana,H.,Kanazawa,H.,Hayashi,K.,and Sekiya,T.: "Detechtion of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms" Proc.Natl.Acad.Sci.,. vol.86,. 2766-2770 (1989)
Description
「研究成果報告書概要(和文)」より
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[Publications] Teshima, S., Shimosato, Y., Hirohashi, S., Tome, Y., Hayashi, I., Kanazawa, H., and Kakizoe, T.: "Four new human germ cell tumor cell lines" Laboratory Investigation (1988) vol.59, 328-336.
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yoshida, T., Tsutsumi, M., Sakamoto, H., Miyagawa, K., Teshima, S., Sugimura, T., and Terada, M.: "Expression of HST1 oncogen in human germ cell tumors" Biochem. Biophys. Res. Commun., (1988) vol. 155, 1324-1329.
Description
「研究成果報告書概要(欧文)」より
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[Publications] Orita, M., Iwahana, H., Kanazawa, H., Hayashi, K., and Sekiya, T.: "Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms" Proc.Natl.Acad.Sci., (1989) vol. 86, 2766-2770.
Description
「研究成果報告書概要(欧文)」より
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[Publications] Fujiki, H., Suganuma, M., Yoshizawa, S., Kanazawa, H., Sugimura, T., Manam, S., Kahn, S.M., Jiang, W., Hoshina, S., and Weinstein, I.B.: "Codon 61 mutations in the c-Harvey-ras gene in mouse skin tumors induced by 7, 12-dimethylbenz(a)anthracene plus okadaic acid class tumor promoters" Molecular carcinogenesis, (1989) vol. 2, 184-187.
Description
「研究成果報告書概要(欧文)」より
