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1989 Fiscal Year Final Research Report Summary

RESEARCH AND DEVELOPMENT ON EXTREMELY PRECISE ACTUATOR SYSTEM FOR CELL BIOTECHNOLOGY APPLICATION USING LASER LIGHT

Research Project

Project/Area Number 62850072
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field 計測・制御工学
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

INABA Humio  Tohoku Univ. RIEC, Professor, 電気通信研究所, 教授 (90006213)

Co-Investigator(Kenkyū-buntansha) SATO Shunichi  Tohoku Univ. RIEC, Research Associate, 電気通信研究所, 助手 (30162431)
ITO Hiromasa  Tohoku Univ. RIEC, Assistant Professor, 電気通信研究所, 助教授 (20006274)
Project Period (FY) 1987 – 1989
KeywordsLaser microscope / Biological cell / Cell fusion / Micro-processing / Semiconductor laser / Optical trapping / Excimer laser / Focused laser beam
Research Abstract

This project aims the research and development of extremely precise actuator system for biotechnology and lifescience applications utilizing diffraction-limited focused laser beam to achieve non-contact micro-processing, trapping. transfer and others of a single biological cell. For this purpose, we newly developed highly precise micro-processing system using ultraviolet pulsed excimer laser,and the single beam optical trapping technique for a trapping and transfer of a single biological cell using continuous-wave laser beam. In the highly precise laser micro-processing system. anultraviolet pulsed laser beam which vas focused upto the diffraction limit by an objective is irradiated on a sample through an inverted-type fluorescence microscope system. The portion of the sample stage is controlled by piezo-electric motor with a resolution less than 1 mum. Thus, based on various experiments for biological cells using this system, we have succeed in demonstrating perforation and cutting of a part of various cells together with cell fusion by means of the laser irradiatim.
Using an Ar laser of 488 nm and semiconductor diode lasers of 0.83 and 1.33 mum, non-contact and non-destructive three-dimensional optical trapping was demonstrated for polystyrene latex spheres and several single biological cells including lymphocyte, erythrocyte, and yeast. It was proved that near-infrared laser light is more suitable for their trapping than visible Ar-laser light because the cell damage caused by laser irradiation is not observed in the case using near-infrared semiconductor diode lasers.
Hence it can be confirmed that this research and development have contributed largely to establish the basic technology for novel highly precise actuator system for single biological cell using laser light which is expected to be extended widely to bioscience and biotechnology in the near future.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 牧野和浩: "生体細胞用レ-ザ-光超精密照射システムの試作開発" 日本レ-ザ-医学会誌. 8. 171-172 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 牧野和浩: "エキシマ-レ-ザ-細胞精密プロセッシング装置を用いた基礎的実験" 日本レ-ザ-医学会誌. 9. 375-378 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐藤俊一: "レ-ザ-光による細胞手術" 細胞工学. 8. 1089-1092 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大弓正志: "単一生体細胞の非接触捕捉・転送のためのレ-ザ-光トラッピング技術" 日本レ-ザ-医学会誌.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐藤俊一: "紫外域レ-ザ-を用いたレ-ザ-細胞融合" 日本レ-ザ-医学会誌.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大弓正志: "半導体レ-ザ-光による生体細胞の非接触トラッピング操作" 医用電子と生体工学.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Makino: "Research and development of extremely precise laser irradiation system for single biological cell." Jpn. J. Laser Med.vol.8. 171-172 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Makino: "Basic research on precise cell micro-processing using excimer laser." Jpn. J. Laser Med.vol.9. 375-378 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Sato: "Laser micro-surgery of Biological Cell" Cell Technology. vol.8. 1089-1092 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Ohyumi: "Laser trapping technique for non-contact trapping and transfer of single biological cell." Jpn. J. Laser Med.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Sato: "Laser biological cell fusion using ultraviolet laser." Jpn. J. Laser Med.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Ohyumi: "Non-contact trapping manipulation of biological cell using semiconductor diode laser." Jpn. J. Electron. Biol. Eng.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-26  

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