1989 Fiscal Year Annual Research Report

大腸菌のゲノム全構造の解明

Research Project

Project/Area Number	63300011
Research Institution	Kobe University
Principal Investigator	磯野克己神戸大学, 理学部, 教授 (70011640)
Co-Investigator(Kenkyū-buntansha)	大坪栄一東京大学, 応用微生物研究所, 教授 (10158800) 中田篤男大阪大学, 微生物病研究所, 教授 (80029769) 井口八郎京都大学, 理学部, 助教授 (20028195) 石浜明国立遺伝学研究所, 分子遺伝部, 教授 (80019869) 由良隆京都大学, ウイルス研究所, 教授 (20027311)
Keywords	大腸菌ゲノム / 整列クロ-ン / 大腸菌デ-タベ-ス / 塩基配列決定 / 遺伝子機能解析
Research Abstract	大腸菌は遺伝学的に最も詳細に解明されてきた生物であり、現在その全ゲノムの約25%の塩基配列が決定されている。しかし、これまでに塩基配列決定のなされたほとんどの部位は遺伝子およびその近傍の領域であり、遺伝子間の構造的特徴やゲノムの最小構成などについてはまだ不明の点が多い。そこで本研究は昭和62年度に発表されたKohara,Akiyama&Isono(Cell 50,495-508,1987)の大腸菌の「整列クロ-ンバンク」を活用し、大腸菌の全ゲノム構造を解明することを究極の目的として企画された。本研究ではこの目標を達成するための種々の方法や技術を検討し、同時にそれらを使って実際にゲノム構造の解明に向けての解析を行ない、さらにこの目標を達成するためには不可欠とえられる共同研究体制のあり方を検討した。また、「大腸菌のデ-タベ-ス」を作成し、国内外の研究者およびそれらの研究者の研究動向を把握すること、およびより高効率なDNAの塩基配列決定法を確立すること、などに的を絞って検討を加えた。本年度から重点領域研究「大腸菌のゲノム全構造の解析」が発足したため、本研究はこの重点研究と重複する形となったが、重点領域研究の総括班と協力して上述した諸点の実現を図った。本研究および上述の重点領域研究により、遺伝子の存在の有無とは無関係に構造解析が行われつつあるので、今後、(1)そのような領域にも遺伝子が存在するのか、(2)存在するとすればどのような遺伝子なのか、(3)大腸菌のDNA中に機能をもたない「無意味な」領域があるのか、(4)ゲノムの構造を特徴づけるものは何か、などの「ゲノム解析」を行なっていくことが可能となるであろう。

Research Products

(69 results)

All Other

All Publications (69 results)

[Publications] Nakamura,H.: "Nucleotide sequence of the cybB gene encoding cytochrome b561 in Escherichia" Mol.Gen.Genet.212. 1-5 (1988)
[Publications] Yamamoto,I.: "Mapping and disruption of the cybB gene coding for cytochrome b561 in Escherichia coli K-12" FEMS Microbiol.Lett.56. 21-28 (1988)
[Publications] Nakao,T.: "Mapping of the multiple regulatory sites for putP and putA expression in the putC region of Escherichia K-12." Mol.Gen.Genet.214. 379-388 (1988)
[Publications] Ohsawa,M.: "Proline carrier mutant of Escherichia coli K-12 with altered cation sensitivity of substrate-binding activity:cloning,biochemical characterization,and identification of the mutation." J.Bacteriol.170. 5185-5191 (1988)
[Publications] Yamato,I.: "Site-specific of cysteine-281,cysteine-344,and cysteine-349 in the proline carrier of Escherichia coli K-12." J.Biol.Cham.263. 16055-16057 (1988)
[Publications] Anraku,Y.: "Bacterial electron transport chains." Ann.Rev.Biochem.57. 101-132 (1988)
[Publications] Deguchi,Y.: "Molecular cloning of gltS and gltP,which encode glutamate carrier of Escherichia coliB." J.Bacteriol.171. 1314-1319 (1989)
[Publications] Kawakami,K.: "Isolation and characterization of herC,a mutation of Escherichia coli affecting maintenance of ColEl." Mol.Gen.Genet.219. 333-340 (1989)
[Publications] Masuda Miura,A.: "The gyrase of Escherichia coli participates in the formation of a spontaneous deletion by recA-independent recombination in vivo." Mol.Gen.Genet.inpress (1990)
[Publications] Glass,R.E.: "Assembly of amber fragments of the β subunit of Escherichia coliRNA polymerase." Eur.J.Biochem.176. 403-407 (1988)
[Publications] Ishihama,A.: "Promoter selectivity of prokaryotic RNA polymerase." Trends in Genet.4. 282-286 (1988)
[Publications] Nomura,T.: "A novel function of RN ase P from Escherichia coli:Processing of a suppressor tRNA precursor." EMBO J.7. 3539-3545 (1988)
[Publications] Ueshima,R.: "DNA supercoiling and temperature shift affect the promoter activity of the Escherichia coli rpoH gene encoding the heat-shock sigma subunit of RNA polymerase." Mol.Gen.Genet.215. 185-189 (1989)
[Publications] Igarashi,K.: "Promoter selectivity of Escherichia coliRNA polymerase:Omega factor is responsible for the ppGpp sensitivity." Nucleic Acids Res.17. 8755-8765 (1989)
[Publications] Wada.A.: "Structure and gene map of a ribosome modulation factor associated with 100S ribosome in stationary-phase Escherichia coli." Proc.Natl.Acad.sci.USA.inpress (1990)
[Publications] Ishihama,A.: "Molecular assembly and functional modulation of Escherichia coliRNA polymerase." Adv.Biophys.26. inpress (1990)
[Publications] Kang,W.K.: "Characterization of the gene rimK responsible for the addition of glutamic acid residues to the C-terminus of ribosomal protein S6 in Escherichia coli." Mol.Gen.Genet.217. 281-288 (1989)
[Publications] Tanaka,S.: "Cloning and molecular characterization fof the gene rimL which encodes an enzyme acetylating ribosomal protein L12 of Escherichia coli." Mol.Gen.Genet.217. 289-293 (1989)
[Publications] Tanaka,Y.: "Cloning and analysis of an Escherichia coli operon containing the rpmF gene for ribosomal protein L32 and the gene for a 30kilodalton protein." J.Bacteriol.171. 5707-5712 (1989)
[Publications] Yamao,F.: "Mischarging mutants of su+2 glutamine tRNA in E.Coli.I.Mutations near the anticodon cause mischarging." Jpn.J.Genet.63. 237-249 (1988)
[Publications] Yamao,F.: "Mischarging mutants of su+2 glutamine tRNA in E.Coli.II.Amino acid specificities of the mutant tRNAs." Jpn.J.Genet.63. 251-258 (1988)
[Publications] Kondo,K.: "The transporsable element Tn3 promotes general recombiation at the neightboring regions." Jpn.J.Genet.64. 417-434 (1989)
[Publications] Komine,Y.: "Genomic organization and physical mapping of the transfer RNA genes in Escherichia coli K-12" J.Mol.Biol.211. inpress (1990)
[Publications] Ohki,R.: "A complete deletion mutant of the Escherichia coli dnaK dnaJ operon." Biochim.Biophys.Acta. 1009. 94-98 (1989)
[Publications] Kawakami,K.: "Isolation and characterization of herC,a mutation of Escherichia coli affecting maintenance of ColEl." Mol.Gen.Genet.219. 333-340 (1989)
[Publications] Umeda,M.: "Mapping of insertion elements IS1.IS2.and IS3 on the E.coliK-12 chromosome;Role of insertion elements in formation of Hfrs and F-prime factors and in rearrangement of bacterial chromosomes." J.Mol.Biol.208. 601-614 (1989)
[Publications] Umeda,M.: "Mapping of an insertion elements IS5.in the E.coliK-12 chromosome;Role of IS5 in rearrangement of the bacterial chromosomes." J.Mol.Biol.inpress (1990)
[Publications] Nozaki,N.: "In vitro transcription of the origin regin of replication of the Escherichia coli chromosome." J.Biol.Chem.263. 14176-14183 (1988)
[Publications] Wada,M.: "Construction and characterization of the delection mutant of hupA and hupB genes in Escherichia coli." J.Mol.Biol.204. 581-591 (1988)
[Publications] Ogawa,T.: "DNA replication in Escherichia coli mutant that lack protein HU." J.Bacteriol.171. 5672-5679 (1989)
[Publications] Aoyama,T.: "Supercoiling responese of E.coli promoters with different spacer lengths." Biochem.Biophys.Acta.949. 311-317 (1988)
[Publications] Kita,K.: "The FokI restriction-modification system.I.Organization and nucleotide sequences of the restriction and modification genes." J.Biol.Chem.264. 5751-5756 (1989)
[Publications] Tabata,S.: "Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome." J.Bacteriol.171. 1214-1218 (1989)
[Publications] Kimura,T.: "Methylation strongly enhances DNA beading the replication origin region of the Escherichia coli chromosome." Mol.Gen.Genet.219. 69-74 (1989)
[Publications] Makino,K.: "Regulation of the phosphate regulon of Escherichia coli.Acitivation of pstS transcription by PhoB protein in vitro." J.Mol.Biol.203. 85-95 (1988)
[Publications] Shinagawa,H.: "RecA protein-dependent cleavage of UmuD protein and SOS mutagenesis." Proc.Natl.Acad.Sci.USA. 85. 1806-1810 (1988)
[Publications] Shinagawa,H.: "Structure and regulation of the Escherichia coli ruv operon involved in DNA repair and recombination." J.Bacteriol.170. 4322-4329 (1988)
[Publications] Kimura,S.: "Regulation of the phosphate regulon of Escherichia coli:characterization of the promoter of the pstS gene." Mol.Gen.Genet.215. 374-380 (1989)
[Publications] Kasahara,M.: "Nucleotide sequence of the ugpQ gene encoding glycerophosphoryl diester phosphodiesterase of Escherichia coli K-12" Nucleic Acids Res.17. 2854 (1989)
[Publications] Nakata,A.: "Unusual nucleotide arrangement with repeated sequences in Escherichia coli K-12 chromosome." J.Bacteriol.171. 3553-3556 (1989)
[Publications] Tao,K.: "Molecular cloning and nucleotide sequence of oxyR,the positive regulatory gene for the adaptive response to oxidative stress in Escherichia coli:Homologies H.between OxyR protein and family of a bacterial activator proteins." Mol.Gen.Genet.218. 371-376 (1989)
[Publications] Iwasaki,H.: "Overproduction,purification,and ATPase activity of the Escherichia coli RuvB protein involved in DNA repair." J.Bacteriol.171. 5276-5280 (1989)
[Publications] Yamada,M.: "Regulation of the phosphate regulon of Escherichia coli:analysis of mutant phoB and phoR genes causing different phenotypes." J.Bacteriol.171. 5601-5606 (1989)
[Publications] Iwasaki,H.: "Involvement DNA repair of the ruvA gene of Escherichia coli." Mol.Gen.Genet.219. 328-331 (1989)
[Publications] Makino,K.: "Signal transduction in the phosphate regulon of Escherichia coli involves phosphotransfer between PhoR and PhoB proteins." J.Mol.Biol.210. 551-559 (1989)
[Publications] Yamada,M.: "Regulation of phosphate regulon of Escherichia coli:properties of phoR deletion mutants subcellular localization of phoR protein." Mol.Gen.Genet.inpress (1990)
[Publications] Nagata,T.: "Requirement the Escherichia coli dnaA gene function of dnaA mutations by plasmid R100-1." Mol.Gen.Genet.213. 163-165 (1988)
[Publications] Katayama,T.: "Genetic suppression of a dnaG mutation in Escherichia coli." J.Bacteriol.171. 1485-1491 (1989)
[Publications] Takasuga,A: "Identification of the penicillin-binding site of penicillin-binding protein 2 of Escherichia coli." J.Biochem.104. 822-826 (1988)
[Publications] Tsuruoka,T.: "Thermosensitive omsA mutation of Escherichia coli that causes thermoregulated release of periplasmic proteins." J.Bacteriol.170. 5229-5235 (1988)
[Publications] Doi,M.: "Determinations of the DNA sequence of the mreB gene and of the gene products of the mre region that function in formation of the rod sphape of Escherichia coli cells." J.Bacteriol.170. 4619-4624 (1988)
[Publications] Wachi,M.: "Negative control of cell division by mreB,a gene that functions in determining the rod shape of Escherichia coli cells." J.Bacteriol.171. 3123-3127 (1989)
[Publications] Ishino,F.: "New mutations fts-36,Its-36,and ftsW clustered in the mra region of the Escherichia coli chromosome induce thermosensitive cell growth and division." J.Bacteriol.171. 5523-5530 (1989)
[Publications] Ikeda,M.: "Structural similarity among Escherichia coli FtsW and RodA proteins and Bacillus subtilis SpoVE protein,which function in cell division, cell elongation,and spore formation,respectively." J.Bacteriol.171. 6375-6378 (1989)
[Publications] Jung,H.K.: "Inhibition of growth of ftsQ,ftsA,and ftsZ mutant cells of Escherichia coli by amplification of a chromosomal region encompassing closely aligned cell division and cell growth genes." J.Bacteriol.171. 6379-6382 (1989)
[Publications] Wachi,M.: "New mre gene mreC,and mreD,responsible for formations of the rod sphape of Escherichia coli cells." J.Bacteriol.171. 6511-6516 (1989)
[Publications] Matsuhashi,M.: "Machinery for cell growth and division:penicillin-binding proteins and other proteins." Research in Microbiology(Paris). January issue.(1990)
[Publications] Ikeda,M.: "Nucleotide sequence involving murD and an open reading frame ORF-V spacing murF and ftsW in Escherichia coli." Nucleic Acids Res.18. inpress (1990)
[Publications] Sampei,G: "Nucleotide sequence of the Escherichia coli purF gene encoding amidophosphoribosyltranferase for de novo purine nucleotide synthesis." Nucleic Acids.Res.16. 8717 (1988)
[Publications] Watanabe,W.: "Identification and sequence analysis of Escherichia coli purE and purK genes encoding 5'-phosphoribosyl-5-amino-4-imidazole carboxylase for de novo purine biosynthesis." J.Bacteriol.171. 198-204 (1989)
[Publications] Sampei,G: "The organization of the purL gene encoding5'-phosphoribosylformylglycinamide amidotransferase of Escherichia coli." J.Biol.Chem.264. 21230-21238 (1989)
[Publications] Aida,A.: "Nucleotide sequence analysis of genes purH and purD involved in the de novo purine nucleotide biosynthesis of Escherichia coli." J.Biol.Chem.264. 21239-21246 (1989)
[Publications] Kusukawa,N.: "Heat shock protein GroE of Escherichia coli.:key protective roles against thermal stress." Genes&Development. 2. 874-882 (1988)
[Publications] Kawasaki,Y.: "Roles of Escherichia coli heat shock proteins DnaK,and GrpE in mini-F plasmid replication." Mol.Gen.Genet.220. 277-282 (1989)
[Publications] Kusukawa,N.: "Effects of mutations in heat-shock genes groES and groEL on protein export in Escherichia coli." EMBOJ.8. 3517-3521 (1989)
[Publications] Yano,R.: "Suppression of the Escherichia coli rpoH opal mutation by ribosomes lacking S15 protein." J.Bacteriol.171. 1712-1717 (1989)
[Publications] Yano,R.: "Mutation that enhances the synthesis of s32 and suppresses the temperature-sensitive growth of the rpoH15 mutant of Escherichia coli." J.Bacteriol.172. (1990)
[Publications] Nagai.H.: "Transcriptiknal regulation of the heat-shock regulatory gene(rpoH)in Escherichia coli:Involvement of a novel catabolite-sensitive promoter." J.Bacteriol.(1990)
[Publications] Anraku,Y.: "Molecular structure and energy coupling mechanism of the Escherichia coli proline carrier,In molecular basis of biomembrane transport,eds.F.Palmieri and E.Quagliariello." Elsevier Scientific Publishers,Amsterdam., 25-32 (1988)

1989 Fiscal Year Annual Research Report

大腸菌のゲノム全構造の解明

Principal Investigator

磯野 克己 神戸大学, 理学部, 教授 (70011640)

Research Products

[Publications] Nakamura,H.: "Nucleotide sequence of the cybB gene encoding cytochrome b561 in Escherichia" Mol.Gen.Genet.212. 1-5 (1988)

[Publications] Yamamoto,I.: "Mapping and disruption of the cybB gene coding for cytochrome b561 in Escherichia coli K-12" FEMS Microbiol.Lett.56. 21-28 (1988)

[Publications] Nakao,T.: "Mapping of the multiple regulatory sites for putP and putA expression in the putC region of Escherichia K-12." Mol.Gen.Genet.214. 379-388 (1988)

[Publications] Ohsawa,M.: "Proline carrier mutant of Escherichia coli K-12 with altered cation sensitivity of substrate-binding activity:cloning,biochemical characterization,and identification of the mutation." J.Bacteriol.170. 5185-5191 (1988)

[Publications] Yamato,I.: "Site-specific of cysteine-281,cysteine-344,and cysteine-349 in the proline carrier of Escherichia coli K-12." J.Biol.Cham.263. 16055-16057 (1988)

[Publications] Anraku,Y.: "Bacterial electron transport chains." Ann.Rev.Biochem.57. 101-132 (1988)

[Publications] Deguchi,Y.: "Molecular cloning of gltS and gltP,which encode glutamate carrier of Escherichia coliB." J.Bacteriol.171. 1314-1319 (1989)

[Publications] Kawakami,K.: "Isolation and characterization of herC,a mutation of Escherichia coli affecting maintenance of ColEl." Mol.Gen.Genet.219. 333-340 (1989)

[Publications] Masuda Miura,A.: "The gyrase of Escherichia coli participates in the formation of a spontaneous deletion by recA-independent recombination in vivo." Mol.Gen.Genet.inpress (1990)

[Publications] Glass,R.E.: "Assembly of amber fragments of the β subunit of Escherichia coliRNA polymerase." Eur.J.Biochem.176. 403-407 (1988)

[Publications] Ishihama,A.: "Promoter selectivity of prokaryotic RNA polymerase." Trends in Genet.4. 282-286 (1988)

[Publications] Nomura,T.: "A novel function of RN ase P from Escherichia coli:Processing of a suppressor tRNA precursor." EMBO J.7. 3539-3545 (1988)

[Publications] Ueshima,R.: "DNA supercoiling and temperature shift affect the promoter activity of the Escherichia coli rpoH gene encoding the heat-shock sigma subunit of RNA polymerase." Mol.Gen.Genet.215. 185-189 (1989)

[Publications] Igarashi,K.: "Promoter selectivity of Escherichia coliRNA polymerase:Omega factor is responsible for the ppGpp sensitivity." Nucleic Acids Res.17. 8755-8765 (1989)

[Publications] Wada.A.: "Structure and gene map of a ribosome modulation factor associated with 100S ribosome in stationary-phase Escherichia coli." Proc.Natl.Acad.sci.USA.inpress (1990)

[Publications] Ishihama,A.: "Molecular assembly and functional modulation of Escherichia coliRNA polymerase." Adv.Biophys.26. inpress (1990)

[Publications] Kang,W.K.: "Characterization of the gene rimK responsible for the addition of glutamic acid residues to the C-terminus of ribosomal protein S6 in Escherichia coli." Mol.Gen.Genet.217. 281-288 (1989)

[Publications] Tanaka,S.: "Cloning and molecular characterization fof the gene rimL which encodes an enzyme acetylating ribosomal protein L12 of Escherichia coli." Mol.Gen.Genet.217. 289-293 (1989)

[Publications] Tanaka,Y.: "Cloning and analysis of an Escherichia coli operon containing the rpmF gene for ribosomal protein L32 and the gene for a 30kilodalton protein." J.Bacteriol.171. 5707-5712 (1989)

[Publications] Yamao,F.: "Mischarging mutants of su+2 glutamine tRNA in E.Coli.I.Mutations near the anticodon cause mischarging." Jpn.J.Genet.63. 237-249 (1988)

[Publications] Yamao,F.: "Mischarging mutants of su+2 glutamine tRNA in E.Coli.II.Amino acid specificities of the mutant tRNAs." Jpn.J.Genet.63. 251-258 (1988)

[Publications] Kondo,K.: "The transporsable element Tn3 promotes general recombiation at the neightboring regions." Jpn.J.Genet.64. 417-434 (1989)

[Publications] Komine,Y.: "Genomic organization and physical mapping of the transfer RNA genes in Escherichia coli K-12" J.Mol.Biol.211. inpress (1990)

[Publications] Ohki,R.: "A complete deletion mutant of the Escherichia coli dnaK dnaJ operon." Biochim.Biophys.Acta. 1009. 94-98 (1989)

[Publications] Kawakami,K.: "Isolation and characterization of herC,a mutation of Escherichia coli affecting maintenance of ColEl." Mol.Gen.Genet.219. 333-340 (1989)

[Publications] Umeda,M.: "Mapping of insertion elements IS1.IS2.and IS3 on the E.coliK-12 chromosome;Role of insertion elements in formation of Hfrs and F-prime factors and in rearrangement of bacterial chromosomes." J.Mol.Biol.208. 601-614 (1989)

[Publications] Umeda,M.: "Mapping of an insertion elements IS5.in the E.coliK-12 chromosome;Role of IS5 in rearrangement of the bacterial chromosomes." J.Mol.Biol.inpress (1990)

[Publications] Nozaki,N.: "In vitro transcription of the origin regin of replication of the Escherichia coli chromosome." J.Biol.Chem.263. 14176-14183 (1988)

[Publications] Wada,M.: "Construction and characterization of the delection mutant of hupA and hupB genes in Escherichia coli." J.Mol.Biol.204. 581-591 (1988)

[Publications] Ogawa,T.: "DNA replication in Escherichia coli mutant that lack protein HU." J.Bacteriol.171. 5672-5679 (1989)

[Publications] Aoyama,T.: "Supercoiling responese of E.coli promoters with different spacer lengths." Biochem.Biophys.Acta.949. 311-317 (1988)

[Publications] Kita,K.: "The FokI restriction-modification system.I.Organization and nucleotide sequences of the restriction and modification genes." J.Biol.Chem.264. 5751-5756 (1989)

[Publications] Tabata,S.: "Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome." J.Bacteriol.171. 1214-1218 (1989)

[Publications] Kimura,T.: "Methylation strongly enhances DNA beading the replication origin region of the Escherichia coli chromosome." Mol.Gen.Genet.219. 69-74 (1989)

[Publications] Makino,K.: "Regulation of the phosphate regulon of Escherichia coli.Acitivation of pstS transcription by PhoB protein in vitro." J.Mol.Biol.203. 85-95 (1988)

[Publications] Shinagawa,H.: "RecA protein-dependent cleavage of UmuD protein and SOS mutagenesis." Proc.Natl.Acad.Sci.USA. 85. 1806-1810 (1988)

[Publications] Shinagawa,H.: "Structure and regulation of the Escherichia coli ruv operon involved in DNA repair and recombination." J.Bacteriol.170. 4322-4329 (1988)

[Publications] Kimura,S.: "Regulation of the phosphate regulon of Escherichia coli:characterization of the promoter of the pstS gene." Mol.Gen.Genet.215. 374-380 (1989)

[Publications] Kasahara,M.: "Nucleotide sequence of the ugpQ gene encoding glycerophosphoryl diester phosphodiesterase of Escherichia coli K-12" Nucleic Acids Res.17. 2854 (1989)

[Publications] Nakata,A.: "Unusual nucleotide arrangement with repeated sequences in Escherichia coli K-12 chromosome." J.Bacteriol.171. 3553-3556 (1989)

[Publications] Tao,K.: "Molecular cloning and nucleotide sequence of oxyR,the positive regulatory gene for the adaptive response to oxidative stress in Escherichia coli:Homologies H.between OxyR protein and family of a bacterial activator proteins." Mol.Gen.Genet.218. 371-376 (1989)

[Publications] Iwasaki,H.: "Overproduction,purification,and ATPase activity of the Escherichia coli RuvB protein involved in DNA repair." J.Bacteriol.171. 5276-5280 (1989)

[Publications] Yamada,M.: "Regulation of the phosphate regulon of Escherichia coli:analysis of mutant phoB and phoR genes causing different phenotypes." J.Bacteriol.171. 5601-5606 (1989)

[Publications] Iwasaki,H.: "Involvement DNA repair of the ruvA gene of Escherichia coli." Mol.Gen.Genet.219. 328-331 (1989)

[Publications] Makino,K.: "Signal transduction in the phosphate regulon of Escherichia coli involves phosphotransfer between PhoR and PhoB proteins." J.Mol.Biol.210. 551-559 (1989)

[Publications] Yamada,M.: "Regulation of phosphate regulon of Escherichia coli:properties of phoR deletion mutants subcellular localization of phoR protein." Mol.Gen.Genet.inpress (1990)

[Publications] Nagata,T.: "Requirement the Escherichia coli dnaA gene function of dnaA mutations by plasmid R100-1." Mol.Gen.Genet.213. 163-165 (1988)

[Publications] Katayama,T.: "Genetic suppression of a dnaG mutation in Escherichia coli." J.Bacteriol.171. 1485-1491 (1989)

[Publications] Takasuga,A: "Identification of the penicillin-binding site of penicillin-binding protein 2 of Escherichia coli." J.Biochem.104. 822-826 (1988)

[Publications] Tsuruoka,T.: "Thermosensitive omsA mutation of Escherichia coli that causes thermoregulated release of periplasmic proteins." J.Bacteriol.170. 5229-5235 (1988)

[Publications] Doi,M.: "Determinations of the DNA sequence of the mreB gene and of the gene products of the mre region that function in formation of the rod sphape of Escherichia coli cells." J.Bacteriol.170. 4619-4624 (1988)

[Publications] Wachi,M.: "Negative control of cell division by mreB,a gene that functions in determining the rod shape of Escherichia coli cells." J.Bacteriol.171. 3123-3127 (1989)

[Publications] Ishino,F.: "New mutations fts-36,Its-36,and ftsW clustered in the mra region of the Escherichia coli chromosome induce thermosensitive cell growth and division." J.Bacteriol.171. 5523-5530 (1989)

[Publications] Ikeda,M.: "Structural similarity among Escherichia coli FtsW and RodA proteins and Bacillus subtilis SpoVE protein,which function in cell division, cell elongation,and spore formation,respectively." J.Bacteriol.171. 6375-6378 (1989)

[Publications] Jung,H.K.: "Inhibition of growth of ftsQ,ftsA,and ftsZ mutant cells of Escherichia coli by amplification of a chromosomal region encompassing closely aligned cell division and cell growth genes." J.Bacteriol.171. 6379-6382 (1989)

[Publications] Wachi,M.: "New mre gene mreC,and mreD,responsible for formations of the rod sphape of Escherichia coli cells." J.Bacteriol.171. 6511-6516 (1989)

[Publications] Matsuhashi,M.: "Machinery for cell growth and division:penicillin-binding proteins and other proteins." Research in Microbiology(Paris). January issue.(1990)

[Publications] Ikeda,M.: "Nucleotide sequence involving murD and an open reading frame ORF-V spacing murF and ftsW in Escherichia coli." Nucleic Acids Res.18. inpress (1990)

[Publications] Sampei,G: "Nucleotide sequence of the Escherichia coli purF gene encoding amidophosphoribosyltranferase for de novo purine nucleotide synthesis." Nucleic Acids.Res.16. 8717 (1988)

[Publications] Watanabe,W.: "Identification and sequence analysis of Escherichia coli purE and purK genes encoding 5'-phosphoribosyl-5-amino-4-imidazole carboxylase for de novo purine biosynthesis." J.Bacteriol.171. 198-204 (1989)

[Publications] Sampei,G: "The organization of the purL gene encoding5'-phosphoribosylformylglycinamide amidotransferase of Escherichia coli." J.Biol.Chem.264. 21230-21238 (1989)

[Publications] Aida,A.: "Nucleotide sequence analysis of genes purH and purD involved in the de novo purine nucleotide biosynthesis of Escherichia coli." J.Biol.Chem.264. 21239-21246 (1989)

[Publications] Kusukawa,N.: "Heat shock protein GroE of Escherichia coli.:key protective roles against thermal stress." Genes&Development. 2. 874-882 (1988)

[Publications] Kawasaki,Y.: "Roles of Escherichia coli heat shock proteins DnaK,and GrpE in mini-F plasmid replication." Mol.Gen.Genet.220. 277-282 (1989)

[Publications] Kusukawa,N.: "Effects of mutations in heat-shock genes groES and groEL on protein export in Escherichia coli." EMBOJ.8. 3517-3521 (1989)

[Publications] Yano,R.: "Suppression of the Escherichia coli rpoH opal mutation by ribosomes lacking S15 protein." J.Bacteriol.171. 1712-1717 (1989)

[Publications] Yano,R.: "Mutation that enhances the synthesis of s32 and suppresses the temperature-sensitive growth of the rpoH15 mutant of Escherichia coli." J.Bacteriol.172. (1990)

[Publications] Nagai.H.: "Transcriptiknal regulation of the heat-shock regulatory gene(rpoH)in Escherichia coli:Involvement of a novel catabolite-sensitive promoter." J.Bacteriol.(1990)

[Publications] Anraku,Y.: "Molecular structure and energy coupling mechanism of the Escherichia coli proline carrier,In molecular basis of biomembrane transport,eds.F.Palmieri and E.Quagliariello." Elsevier Scientific Publishers,Amsterdam., 25-32 (1988)

磯野克己神戸大学, 理学部, 教授 (70011640)