"Structural Analysis of the Whole Genome of Escherichia coli"

1989 Fiscal Year Final Research Report Summary

• Project/Area Number: 63300011
• Research Category: Grant-in-Aid for Co-operative Research (A)
• Allocation Type: Single-year Grants
• Research Field: 広領域
• Research Institution: Kobe University
• Principal Investigator: ISONO Katsumi Kobe Univ., Faculty of Science Professor, 理学部, 教授 (70011640)
• Co-Investigator(Kenkyū-buntansha): OHTSUBO Eiichi Tokyo Univ., Inst. of Applied Microbiol. Professor, 応用微生物研究所, 教授 (10158800) ; NAKATA Atsuo Osaka Univ., Inst. of Microbial Diseases Professor, 微生物病研究所, 教授 (80029769) ; INOKUCHI Hachiro Kyoto Univ., Faculty of Science Assoc. Prof., 理学部, 助教授 (20028195) ; ISIHAMA Akira National Institute of Genetics Professor, 分子遺伝部, 教授 (80019869) ; YURA Takashi Kyoto Univ., Inst. of Virus Research Professor, ウィルス研究所, 教授 (20027311)
• Project Period (FY): 1988 – 1990
• Keywords: E. coli genome / Ordered clones / E. coli database / Nucleotide sequencing / Functional analysis of genes

Research Abstract

Escherichia coli is one of the most thoroughly investigated organisms and approximately 25 % of its genome have been sequenced till now. However, most of the regions so far sequenced are those containing known structural genes and their flanking sequences. It remains to be investigated what the structural characteristics of the intergenic regions are and what the minimal constituents of a genome are. This project was established to approach such problems by exploiting the "ordered clone bank" of Kohara, Akiyama and Isono (Cell 50: 495-508, 1987). It aimed at analyzing eventually the whole genomic structure of E. coli. To achieve this goal, it was planned to develop and improve various methods required for genome analysis, to apply such methods for determining the nucleotide sequences of long genomic stretches, and to discuss on the necessary collaborations among project members. Furthermore, an "E. coli database" was constructed and distributed among project members, and methods for more efficient nucleotide sequencing were sought. In April, 1989, another project work for E. coli genome analysis was started which is supported by a grant-in-aid for research in priority area from the Ministry of Education. As a result, all members of this project became supported by the two grants. Therefore, every effort was made to achieve effective cooperations between the members of two projects. Since the work performed in this project is yielding sequence data without prior knowledge of existence of any genes, it is anticipated to give us clues with respect to the points mentioned above.