1990 Fiscal Year Final Research Report Summary
Development of Active Transport and Ion-Channel Sensors
| Project/Area Number |
63430008
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
分析・地球化学
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| Research Institution | Hokkaido University |
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Principal Investigator |
UMEZAWA Yoshio Hokkaido University, Faculty of Science, Professor, 理学部, 教授 (80011724)
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| Co-Investigator(Kenkyū-buntansha) |
SUGAWARA Masao Hokkaido University, Faculty of Science, Instructor, 理学部, 助手 (50002176)
ODASHIMA Kazunori Hokkaido University, Faculty of Science, Lecturer, 理学部, 講師 (30152507)
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| Project Period (FY) |
1988 – 1990
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| Keywords | Ion-channel / Active transport / Chemical sensor / Biosensor / Langmuir-Blodgett Membrane / Bilayer lipid membrane |
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| Research Abstract |
Three different types of sensors were constructed. (1) A series of ion-channel type sensors responsive to organic and inorganic anions were constructed utilizing Langmuir-Blodgett membranes containing lipophilic derivatives of macrocyclic polyamine and cyclodextrin polyamine, capable of binding with anionic guests in multiprotonated forms. The sensor showed selective response (modulation of membrane permeability to a number of guest anions including adenosine nucleotides, dicarboxylates and inorganic anions. (2) A biological ion-channel sensor was fabricated by incorporating glutamate receptorion-channel protein, isolated from synaptic membrane of rat brain, into a planar bilayer lipid membrane (BLM) . A signal amplification of 2.1x10^5 was obtained based on the glutamate-triggered single channel current. (3) A Na^+/D-glucose co-transporter protein, isolated from intestinal brush border membrane of guinea pigs, was incorporated into a bilayer membrane to construct an active transport type BLM sensor for D-glucose. The response of the sensor was measured as a conductance change arising from co-transported Na^+ ion flux under a constant applied potential. The response of the sensor was induced by D-glucose above 10^<-9> M, but not by the corresponding L-isomer, D-fructose and D-mannose.
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