1990 Fiscal Year Annual Research Report

細胞分裂酵素・蛋白質系の解析と再構成

Research Project

Project/Area Number	63430020
Research Institution	The University of Tokyo
Principal Investigator	松橋通生東京大学, 応用微生物研究所, 教授 (40013297)
Co-Investigator(Kenkyū-buntansha)	冨田啓子東京大学応用微生物研究所, 教務職員
Keywords	細胞分裂 / 細胞生長 / ペニシリン結合蛋白質 / 細胞壁ペプチドグリカン / メチシリン耐性ブドウ球菌 / 半数体細胞 / 倍数性 / リピド中間体
Research Abstract	最も単純な細胞に於て、生物の基本的な営みである細胞分裂の機構を解明し、細胞増殖の系を再構成することを目的として、以下の研究を行った。 1。大腸菌の細胞生長と分裂に関与する<mra>___ー遺伝子群の解析。大腸菌染色体地図上2分に位置する20キロベ-ス<mra>___ー領域の完全な遺伝子解析をほヾ終了した。<mra>___ー領域の5^1ー末端に近い所に存在する330塩基対のオ-プンリ-ディングフレ-ム中に細胞生長と分裂のそれぞれを欠損させる変異があることを明らかにし、また<mraY>___ー、<murG>___ー遺伝子がペプチドグリカン生合成におけるリピド中間体生成の2つの酵素をコ-ドすることを明らかにした。 2。大腸菌の細胞生長と分裂を制御する<mre>___ー領域(71分)に属する<mreB>___ー、<mreC>___ー、<mreD>___ー遺伝子の上記の制御機能を同定し、また<mreD>___ー遺伝子の下流(3^1ー末端の外側)にあるorfE、orfFの塩基配列を決定し、またそれらの機能を推定した。 3。黄色ブドウ球菌のβーラクタム高度耐性菌(MRSAと略称され、病院感染の大きな問題になっている)の耐性原因と考えられる遺伝子、<mecA>___ーと、その発現を制御すると考えられる<mecB>___ー遺伝子の解析を1960年以降に世界で分離されている様々なMRSA株について行った。 4。マウスの半数体細胞系列を分離する目的で,半数体未受精卵を発生させ、これより遺伝子導入其他の方法で安定に自己増殖する半数体培養細胞を育成することを試み、有用なデ-タ-を得た。 5。細胞骨格系蛋白質乃至細胞骨格様蛋白質の分離と解析をすゝめた。

Research Products
(14 results)

All Other

All Publications (14 results)

[Publications] Kimiko UBUKATA: "Homology of mecA gene in methicillinーresistant Staphylococcus haemolyticus and Staphylococcus simulans to that of Staphylosoccus aureus" Antimicrobial Agents and Chemotherapy. 34. 170-172 (1990)
[Publications] Michio MATSUHASHI: "Machinery for cell growth and division;penicillinーbinding proteins and other proteins" Research in Microbiology,4th Forum in Microbiology. 141. 89-103 (1990)
[Publications] Yumiko SAKAKIBARA: "Effects of synthetic estrogens,(R,R)ー(+)ー,(S,S)ー(-)ー,dlー and mesoーhexestrol stereoisomers on microtubule assembly" Biochemical Pharmacology. 39. 167-171 (1990)
[Publications] Masato IKEDA: "Nucleotide sequence involving murG and murC in the mra gene cluster region of Escherichia coli" Nucleic Acids Research. 18. 4014-4014 (1990)
[Publications] Fumitoshi ISHINO: "Developmental potential of haploidーderived parthenogenetic cells in mouse chimeric embryos" Development Growth & Differentiation. 32. 139-144 (1990)
[Publications] Ayumu YAMAMOTO: "Solubilization of asterーforming proteins from yeast:possible constituents of spindle pole body and reconstitution of asters in vitro" Cell Structure and Function. 15. 221-228 (1990)
[Publications] Toshihiko MONODANE: "Comparison of two kinds of cell packets of Micrococcus luteus by scanning electron microscopy:outermost layer maintaining the packet structure" Microbiol.Immunology. 34. 691-696 (1990)
[Publications] Fumitoshi ISHINO: "Mutation causing overproduction of the short peptide component gamma of penicillinーbinding protein lBs of Escherichia coli" Journal of General and Applied Microbiology. 36. 163-169 (1990)
[Publications] Masato IKEDA: "Homology among MurC,MurD,MurE and MurF proteins in Escherichia coli and that between E.coli MurG and a possible MurG protein in Bacillus subtilis" Journal of General and Applied Microbiology. 36. 179-187 (1990)
[Publications] Michihiko ITO: "High level of aphidicolin resistance with multiple mutations in mouse FM3A cell mutants" Somatic Cell and Molecular Genetics. 16. 443-450 (1990)
[Publications] Yumiko SAKAKIBARA: "Effect of mesoーhexestrol,a synthetic estrogen,on Stubulin" Chemical Pharmacology Bulletin. 38. 3419-3422 (1990)
[Publications] Masato IKEDA: "The Escherichia coli mraY gene encoding UDPーNーacetylmuramoylーpentapeptide:undecaprenylーphosphate phosphoーNーacetylmuramoylーpentapeptide transferase" Journal of Bacteriology. 173. (1991)
[Publications] Michio MATSUHASHI: "“50 Years of Penicillin Application," Functions of penicillinーbinding proteins and their regulation" (1990)
[Publications] Michio MATSUHASHI: "“50 Years of Penicillin Application," Septum peptidoglycan sunthesis in vitro in Escherichia coli;reconstitution of the PBP3ーFtsW system on the membranes" (1990)

1990 Fiscal Year Annual Research Report

細胞分裂酵素・蛋白質系の解析と再構成

Principal Investigator

松橋 通生 東京大学, 応用微生物研究所, 教授 (40013297)

Research Products

[Publications] Kimiko UBUKATA: "Homology of mecA gene in methicillinーresistant Staphylococcus haemolyticus and Staphylococcus simulans to that of Staphylosoccus aureus" Antimicrobial Agents and Chemotherapy. 34. 170-172 (1990)

[Publications] Michio MATSUHASHI: "Machinery for cell growth and division;penicillinーbinding proteins and other proteins" Research in Microbiology,4th Forum in Microbiology. 141. 89-103 (1990)

[Publications] Yumiko SAKAKIBARA: "Effects of synthetic estrogens,(R,R)ー(+)ー,(S,S)ー(-)ー,dlー and mesoーhexestrol stereoisomers on microtubule assembly" Biochemical Pharmacology. 39. 167-171 (1990)

[Publications] Masato IKEDA: "Nucleotide sequence involving murG and murC in the mra gene cluster region of Escherichia coli" Nucleic Acids Research. 18. 4014-4014 (1990)

[Publications] Fumitoshi ISHINO: "Developmental potential of haploidーderived parthenogenetic cells in mouse chimeric embryos" Development Growth & Differentiation. 32. 139-144 (1990)

[Publications] Ayumu YAMAMOTO: "Solubilization of asterーforming proteins from yeast:possible constituents of spindle pole body and reconstitution of asters in vitro" Cell Structure and Function. 15. 221-228 (1990)

[Publications] Toshihiko MONODANE: "Comparison of two kinds of cell packets of Micrococcus luteus by scanning electron microscopy:outermost layer maintaining the packet structure" Microbiol.Immunology. 34. 691-696 (1990)

[Publications] Fumitoshi ISHINO: "Mutation causing overproduction of the short peptide component gamma of penicillinーbinding protein lBs of Escherichia coli" Journal of General and Applied Microbiology. 36. 163-169 (1990)

[Publications] Masato IKEDA: "Homology among MurC,MurD,MurE and MurF proteins in Escherichia coli and that between E.coli MurG and a possible MurG protein in Bacillus subtilis" Journal of General and Applied Microbiology. 36. 179-187 (1990)

[Publications] Michihiko ITO: "High level of aphidicolin resistance with multiple mutations in mouse FM3A cell mutants" Somatic Cell and Molecular Genetics. 16. 443-450 (1990)

[Publications] Yumiko SAKAKIBARA: "Effect of mesoーhexestrol,a synthetic estrogen,on Stubulin" Chemical Pharmacology Bulletin. 38. 3419-3422 (1990)

[Publications] Masato IKEDA: "The Escherichia coli mraY gene encoding UDPーNーacetylmuramoylーpentapeptide:undecaprenylーphosphate phosphoーNーacetylmuramoylーpentapeptide transferase" Journal of Bacteriology. 173. (1991)

[Publications] Michio MATSUHASHI: "“50 Years of Penicillin Application," Functions of penicillinーbinding proteins and their regulation" (1990)

[Publications] Michio MATSUHASHI: "“50 Years of Penicillin Application," Septum peptidoglycan sunthesis in vitro in Escherichia coli;reconstitution of the PBP3ーFtsW system on the membranes" (1990)

松橋通生東京大学, 応用微生物研究所, 教授 (40013297)