1989 Fiscal Year Final Research Report Summary
Structure and Functions of Immunoglobulins
| Project/Area Number |
63430022
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | Faculty of Pharmaceutical Sciences, University of Tokyo |
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Principal Investigator |
ARATA Yoji Faculty of Pharmaceutical Sciences University of Tokyo, Professor, 薬学部, 教授 (40011499)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIMURA Yoshifumi Yokohama City University Graduate School of Integrated Science, Professor, 大学院総合理学研究科, 教授 (70107390)
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| Project Period (FY) |
1988 – 1989
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| Keywords | NMR / Immunoglobulin / Antigen-antibody interactions |
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| Research Abstract |
The aim of this research is 1) to understand the molecular struetural basis of the antigen recognition by antibody and 2) to develop a general means of providing antibody with a variety of reactivity on the basis of the information obtained. Two-dimensional homonuclear Hartmann-Hahn (2D-HOHAHA) spectroscopy was found to be effective in establishing the connectivity between the C2-H and C4-H protons of His residues in the antibody molecule. The antibody molecule was selectively labeled with ^<13>C at the carbonyl carbon of a variety of amino acid residues including methionine and tryptophan by growing hybridoma cells in serum-free medium. It was possible to observe all the carbonyl carbon resonances of the antibody. On the basis of the results obtained using the technique of stable isotope labeling, structure-function correlates for the antibody molecule have successfully been discussed.
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Research Products
(14 results)
