1989 Fiscal Year Annual Research Report

下顎頭の成長及び老化に関与する局所成長因子の役割

Research Project

Project/Area Number	63440072
Research Institution	Osaka University
Principal Investigator	鈴木不二男大阪大学, 歯学部, 教授 (40028717)
Co-Investigator(Kenkyū-buntansha)	浅田彬大阪大学, 歯学部, 講師 (50028734) 加藤幸夫大阪大学, 歯学部, 講師 (10112062)
Keywords	成長因子 / 下顎頭 / 軟骨 / 老化
Research Abstract	1.CDFの精製牛胎児軟骨の粗抽出物をSDS-PAGEに展開したのち、gelから抽出してCDF活性を測定すると、分子量16,000と24,000に相当する画分に軟骨細胞のプロテオグリカン合成を促進する活性が出現した。そこで、牛胎児軟骨の抽出物を限外濾過、血清蛋白affinity chromatography,hepain-affinity chromatography,Mono-S chromatography,C18-HPLCにて分画して、16K-CDFと24K-CDFを純化した。16K-CDFにはN-glycanseにて分解される糖鎖はなかったが、24K-CDFには分子量4000の糖鎖が結合していた。これらのCDFを^<125>I-ヨ-ドにてラベルして軟骨細胞とincubateすると、これらのCDFは温度、時間依存性に軟骨細胞と特異的に結合した。他の成長因子あるいはホルモンを添加しても^<125>I-CDFの結合は影響されなかった。即ち軟骨細胞にはCDFに対する特異的な受容体が依存することが明らかとなった(日本生化学会、1989)。 2、軟骨に存在する局所成長因子の含量軟骨のinsulin-like growth factor-1,insulin-like growth factor-II,basic fibroblast growth factorの含量をradioimmunoassayおよびradioreceptor assayにて測定する方法を確立した(近畿支部生化学会、1989)。

Research Products
(19 results)

All Other

All Publications (19 results)

[Publications] Masahiro Iwamoto: "Regulation of colony formation of differentiated chondrocytes in soft agar by transformed growth factor-beta" Biochem.Biophys.Res.Commun.159. 1006-1011 (1989)
[Publications] Masahiro Iwamoto: "Hypertrophy and calcification of rabbit permanent chondrocytes in pelleted cultures:synthesis of alkaline phosphatase and 1,25-dihydroxycholecalciferol receptor" Dev.Biol.136. 500-507 (1989)
[Publications] Koike Tatsuya: "Potent mitogenic effects of parathyroid hormone(PTH)on embryonic chick and rabbit chondrocytes:differential effects of age on growth,proteoglycan,and cyclic AMP responses of chondrocytes to PTH" J.Clin.Invest.85. (1990)
[Publications] Katsuhiko Sato: "1,apha,25-dihydroxyvitamin D3 stimulates colony formation of chick embryo chondrocytes in soft agar" Exp.Cell Res.(1990)
[Publications] Yukio Kato: "Fibroblast growth factor is an inhibitor of chondrocyte terminal differentiation" J.Biol.Chem.265. (1990)
[Publications] Yukio Kato: "Rabbit articular chondrocytes in soft agar are useful to test the effect of non-steroidal anti-inflammatory drugs on chondrocyte replication" Scand.J.Rheumatol.77(Suppl.). 3-6 (1989)
[Publications] Yukio Kato: "Terminal differentiation and calcification in rabbit chondrocyte cultures grown in centrifuge tubes:regulation by transforming growth factor-beta and serum factors" Proc.Natl.Acad.Sci.U.S.A.85. 9552-9556 (1988)
[Publications] Yukio Kato: "Effects of limited exposure of rabbit chondrocyte cultures to parathyroid hormone and dibutyryl adenosine 3',5'-monophosphate on cartilage characteristic proteoglycan synthesis" Endocrinology. 122. 1991-1997 (1988)
[Publications] Yuji Hiraki: "Effect of transforming growth factor-beta on cell proliferation and glycosaminoglycan synthesis by rabbit growth-plate chondrocytes in culture" Biochim.Biophys.Acta. 969. 91-99 (1988)
[Publications] Hiroyuki Inoue: "Stimulation of cartilage-matrix proteoglycan synthesis by morphologically transformed chondrocytes grown in the presence of fibroblast growth factor and transforming growth factor-beta" J.Cell.Physiol.138. 329-337 (1989)
[Publications] M.Koji Owada: "Effects of vanadate on tyrosine phosphorylation and the pattern of glycosaminoglycan synthesis in rabbit chondrocytes in culture" J.Cell.Physiol.138. 484-492 (1989)
[Publications] Masahiro Iwamoto: "Interleukin 1:a regulator of chondrocyte proliferation" Immunol.Lett.21. 153-156 (1989)
[Publications] 小池達也: "軟骨細胞無血清軟寒天培養系におけるhigh-density lipoprotein(HDL)の影響" 中部日本整形外科災害外科学会誌. 32. 482-484 (1989)
[Publications] 加藤幸夫: "成長因子とホルモンによる軟骨細胞のterminal differentiationと細胞外基質石灰化の制御" 日本骨代謝学会誌. 7. 16-20 (1989)
[Publications] 加藤幸夫: "骨・軟骨由来成長因子" 日本臨床増刊“Receptor". 373-377 (1981)
[Publications] 加藤幸夫: "成長板軟骨細胞の成熟、肥大、及び石灰化に伴う成長因子とホルモン受容体の変動" 日本骨代謝学会誌. 7. 26-31 (1989)
[Publications] 加藤幸夫: "軟骨細胞の最終分化と石灰化:場による制御" 組織培養. 15. 390-394 (1989)
[Publications] 加藤幸夫: "軟骨細胞の増殖と分化の制御" 実験医学. 7. 1279-1285 (1989)
[Publications] Yukio kato: "Methods in Enzymol.Purification of various growth factors from cartilage" Academic Press Inc.,Publishers New York,

1989 Fiscal Year Annual Research Report

下顎頭の成長及び老化に関与する局所成長因子の役割

Principal Investigator

鈴木 不二男 大阪大学, 歯学部, 教授 (40028717)

Research Products

[Publications] Masahiro Iwamoto: "Regulation of colony formation of differentiated chondrocytes in soft agar by transformed growth factor-beta" Biochem.Biophys.Res.Commun.159. 1006-1011 (1989)

[Publications] Masahiro Iwamoto: "Hypertrophy and calcification of rabbit permanent chondrocytes in pelleted cultures:synthesis of alkaline phosphatase and 1,25-dihydroxycholecalciferol receptor" Dev.Biol.136. 500-507 (1989)

[Publications] Koike Tatsuya: "Potent mitogenic effects of parathyroid hormone(PTH)on embryonic chick and rabbit chondrocytes:differential effects of age on growth,proteoglycan,and cyclic AMP responses of chondrocytes to PTH" J.Clin.Invest.85. (1990)

[Publications] Katsuhiko Sato: "1,apha,25-dihydroxyvitamin D3 stimulates colony formation of chick embryo chondrocytes in soft agar" Exp.Cell Res.(1990)

[Publications] Yukio Kato: "Fibroblast growth factor is an inhibitor of chondrocyte terminal differentiation" J.Biol.Chem.265. (1990)

[Publications] Yukio Kato: "Rabbit articular chondrocytes in soft agar are useful to test the effect of non-steroidal anti-inflammatory drugs on chondrocyte replication" Scand.J.Rheumatol.77(Suppl.). 3-6 (1989)

[Publications] Yukio Kato: "Terminal differentiation and calcification in rabbit chondrocyte cultures grown in centrifuge tubes:regulation by transforming growth factor-beta and serum factors" Proc.Natl.Acad.Sci.U.S.A.85. 9552-9556 (1988)

[Publications] Yukio Kato: "Effects of limited exposure of rabbit chondrocyte cultures to parathyroid hormone and dibutyryl adenosine 3',5'-monophosphate on cartilage characteristic proteoglycan synthesis" Endocrinology. 122. 1991-1997 (1988)

[Publications] Yuji Hiraki: "Effect of transforming growth factor-beta on cell proliferation and glycosaminoglycan synthesis by rabbit growth-plate chondrocytes in culture" Biochim.Biophys.Acta. 969. 91-99 (1988)

[Publications] Hiroyuki Inoue: "Stimulation of cartilage-matrix proteoglycan synthesis by morphologically transformed chondrocytes grown in the presence of fibroblast growth factor and transforming growth factor-beta" J.Cell.Physiol.138. 329-337 (1989)

[Publications] M.Koji Owada: "Effects of vanadate on tyrosine phosphorylation and the pattern of glycosaminoglycan synthesis in rabbit chondrocytes in culture" J.Cell.Physiol.138. 484-492 (1989)

[Publications] Masahiro Iwamoto: "Interleukin 1:a regulator of chondrocyte proliferation" Immunol.Lett.21. 153-156 (1989)

[Publications] 小池達也: "軟骨細胞無血清軟寒天培養系におけるhigh-density lipoprotein(HDL)の影響" 中部日本整形外科災害外科学会誌. 32. 482-484 (1989)

[Publications] 加藤幸夫: "成長因子とホルモンによる軟骨細胞のterminal differentiationと細胞外基質石灰化の制御" 日本骨代謝学会誌. 7. 16-20 (1989)

[Publications] 加藤幸夫: "骨・軟骨由来成長因子" 日本臨床増刊“Receptor". 373-377 (1981)

[Publications] 加藤幸夫: "成長板軟骨細胞の成熟、肥大、及び石灰化に伴う成長因子とホルモン受容体の変動" 日本骨代謝学会誌. 7. 26-31 (1989)

[Publications] 加藤幸夫: "軟骨細胞の最終分化と石灰化:場による制御" 組織培養. 15. 390-394 (1989)

[Publications] 加藤幸夫: "軟骨細胞の増殖と分化の制御" 実験医学. 7. 1279-1285 (1989)

[Publications] Yukio kato: "Methods in Enzymol.Purification of various growth factors from cartilage" Academic Press Inc.,Publishers New York,

鈴木不二男大阪大学, 歯学部, 教授 (40028717)