1991 Fiscal Year Final Research Report Summary
Analysis of gene expression of calpain and calpastatin
| Project/Area Number |
63440083
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Kyoto University |
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Principal Investigator |
NOSAKA Tetsuya Inst. Virus Res, Kyoto University Instructor, ウイルス研究所, 助手 (30218309)
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| Co-Investigator(Kenkyū-buntansha) |
UEDA Kunihiro Kyoto Univ. Fac. Med. Associate Prof., 医学部, 助教授 (00027070)
HATANAKA Masakazu Inst. Virus Res. Kyoto Univ. Professor, ウイルス研究所, 教授 (30142300)
MAKI Masatoshi Inst. Virus Res. Kyoto Univ. Associate Prof, ウイルス研究所, 助教授 (40183610)
ADACHI Yoshifumi Kyoto Univ. Fac. Med. Instructor, 医学部, 助手 (50201893)
MURACHI Takashi Kyoto Univ. Fac. Med. Professor, 医学部, 教授 (10089104)
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| Project Period (FY) |
1988 – 1991
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| Keywords | Calpain / Calpastatin / Proteinase / Inhibitor / HTLV-I |
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| Research Abstract |
Calpain is an intracellular Ca^<2+>-dependent cysteine endopeptidase, and calpastatin is an endogenous specific inhibitor of calpain. Both proteins constitute an intracellular regulatory system operating in response to Ca^<2+> signaling. We cloned the CDNA for pig heart calpastatin and investigated structure-function relationships. The inhibitor consists of four inhibitory domains with mutually homologous sequences. A human calpastatin genomic DNA clone- was isolated, and synthetic oligopeptide corresponding to an exon of the human calpastatin gene showed strong inhibition against calpain I and calpain. When various hematopoietic cells were screened by Westem blot analysis, using antibodies specific for calpain I and for calpain 11, respectively ; HTLV-L infected cells were found to express high level ofcalpain 11, whereas HTLV-l non infected T cells did not express any appreciable amount of calpain 11. Calpain I was expressed in all the cells tested. Calpastatin gene expression was also found to be elevated in HTLV-L infected cells.
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