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1989 Fiscal Year Final Research Report Summary

Regulation of Reproductive Activity by Steroid Hormone Metabolizing Enzymes

Research Project

Project/Area Number 63480089
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 基礎獣医学
Research InstitutionFaculty of Agriculture, The University of Tokyo

Principal Investigator

TAKAHASHI Michio  The Univ. of Tokyo, Veterinary Medical Science, Professor., 農学部, 教授 (30011943)

Co-Investigator(Kenkyū-buntansha) NODA Ken  The Univ. of Tokyo, Veterinary Medical Science, Assistant Professor., 農学部, 助手 (60218287)
SHIOTA Kunio  The Univ. of Tokyo, Veterinary Medical Science, Associate Professor, 農学部, 助教授 (80196352)
Project Period (FY) 1988 – 1989
KeywordsPregnancy / Corpus luteum / Progesterone / 20alpha-hydroxysteroid dehydrogenase / Prolactin / steroid hormone metabolizing enzyme / Ovary / 20alpha-dihydroprogesterone
Research Abstract

20a-hydroxysteroid dehydrogenase (20alpha-HSD) catabolizes progesterone, which plays a crucial role to achieve and maintain pregnancy and is a major precursor of several steroid hormones, into biologically inactive 20a-dihydroprogesterone and plays important roles in steroid metabolism. Especially in rodents, the increase of this enzyme activity in the corpus luteum causes the rapid decrease of peripheral progesterone levels and this mechanism enable rodents to actualize very short ovulation cycle (about 4 days per a cycle). Pregnancy of the rodents is achieved and maintained by the inhibition of this increase in 20alpha-HSD activity by prolactin (PRL). In this research, we studied about this enzyme and the mechanism of the regulation of the activity at cellular and molecular level, and the results are as follows.
1) Ovarian 20alpha-HSDs were completely purified 2) There are two types of 20alpha-HSD with very close molecular weight (33,000) and with different ionic strengths.
3) Anti-20alpha-HSD antiserum were obtained, and cellular and intracellular localization was analyzed by immunocytochemi story and western blotting.
4) We found that suppressive action of PRL on the enzyme activity is mediated by transforming growth factor-beta (TGF-beta) which is produced by luteal macrophages.
5) cDNA library of rat ovary was prepared to get clone of 20alpha-HSD cDNA.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Naito,K.and M.Takahashi: "The effect of peritoneal macrophages' on monolayered luteal cell progestin secretion in the rat." Endocrinologia Japonica. 35. 439-446 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saito,S.,S.Matsuyama,K.Shiota and M.Takahashi: "Involvement of splenocytes' in the control of corpus luteum function in the rat." Endocrinologia Japonica. 35. 891-898 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kadota,C.,M.Ohta,M.Takahashi: "Dynamic response to follicle-stimulating hormone of secretion of progesterone by super tused rat granulosa cells." Molecular and Cellular Endocrinology. 59. 213-220 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuda,J.,K.Noda,K.Shiota,M.Takahashi: "Participation of ovarian 20α-hydroxysteroid dehydragenase in luteotrophic and luteolytic processes during rat pseudopregnancy." Journal of Reproduction and Fertility(印刷中). 88. (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kadota,C.,M.Ohta,M.Takahashi.: "Effects of androgen on progesterone secretion from granulosa cells obtained from antral follicles of rats" Endocrinologia Japonica(印刷中). 37. (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noda,K.,K.Shiota,M.Takahashi: "Durification and characterization of rat ovarian 20α-hydroxysteroid dehydrogenase." Biochemical Journal(投稿中). (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takahashi,M.,F.Kasuga,S.saito,S.Matsuyama,K.yamanouchi,T.Murata,K.Shiota: "Development of Preimplantation Embryo's and Their Environment.(Yoshinaga K,Mori T.Eds.)" Alan R.Liss,Inc, 101-115 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Naito, K. and M. Takahashi: "The effect of peritoneal macrophages on monolayered luteal cell progestin secretion in the rat." Endocrinol. Jpn. 35, 439-446, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito, S., S. Matsuyama, K. Shiota, and M. Takahashi: "Involvement of splenocytes in the control of corpus luteum function in the rat." Endocrinol. Jpn. 35, 891-898, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kadota, T., M. Ohta, M. Takahashi: "Dynamic response to follicle-stimulating hormone of secretion of progesterone by superfused rat ovarian granulosa cells." Mol. Cell. Endocrinol., 59, 213-220, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kasuga, F., K. Shiota, M. Takahashi: "Significance of germ cells for the expression of testicular endocrine function in rats." J. Reprod. Fert., 87, 179-186, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuda, J., K. Noda, K. Shiota and M. Takahashi: "Participation of ovarian 20alpha-hydroxysteroid dehydrogenase in luteotrophic and luteolytic processes during rat pseudopregnancy." J. Reprod. Fert. 88, 1990.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kadota, C., M. Ohta and M. Takahashi: "Effects of androgen on progesterone secretion from granulosa cells obtained from antral follicles of rats." Endocrinol. Jpn. 37, 1990.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noda, K. K. Shiota, M. Takahashi: "Purification and Characterization of Rat Ovarian 20alpha-hydroxysteroid dehydrogenase." Biochem. J. 1990.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-26  

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