1989 Fiscal Year Annual Research Report

正常組織におけるPAFとその内在性インヒビタ-

Research Project

Project/Area Number	63480132
Research Institution	Kansai Medical University
Principal Investigator	斎藤國彦関西医科大学, 医学部, 教授 (80077560)
Co-Investigator(Kenkyū-buntansha)	中山玲子関西医科大学, 医学部, 助手 (60172483) 里内清関西医科大学, 医学部, 講師 (50098125)
Keywords	PAF / アシル型PAF / ビニ-ル型PAF / ケミカル・メジィエ-タ- / オ-タコイド / 正常胃組織 / 正常心組織 / 炎症性胸水
Research Abstract	PAF(血小板活性化因子)は免疫・炎症反応のメジィエ-タ-と見なされ、一般には正常組織には存在しないと考えられていた。然しながら、我々は外界から何らの刺激を加えない正常組織例えばラット子宮にも微量ながらPAFが存在することを初めて報告したが、その生理的意義については全く不明である。今年も引き続き正常組織のPAFについて、また他方、疾病とPAFとの関係について以下の実績を得た。 1.ラットおよびモルモット心筋に存在するPAF類縁化合物についていづれにもPAF、アシル型PAFおよびビニ-ル型PAFが存在し、このビニ-ル型が発見されたのはこれが始めてである。量的にはアシル型が一番多く、次いでビニ-ル型そしてPAFの順であり、いづれも16:0が主成分であった。 2.ラット胃におけるPAF類似化合物について胃では胃前庭、体部、前胃の順に、十二指腸では胃体部より多量にPAFおよびアシル型PAFが存在しており、アルコ-ルの経口投与によって著明に低下した。この際アセチ-ル・ヒドロラ-ゼ活性は上昇していた。正常組織におけるPAFとその関連化合物の意義は不明ではあるが、オ-タコイド様の機能を持っていると考えている。 3.アレルギ-と関係の深い鼻ポリ-プの中に、好酸球および好中球の含まれた胸水の中に、PAFおよびアシル型PAFを検出し同定した。また人単核球は殆ど特異的に尿酸刺激にてPAFを生成した。以上の様にPAFは炎症免疫反応に関係するのみならず、正常組織の機能にも深く係わる重要な生理活性物質である。

Research Products

(18 results)

All Other

All Publications (18 results)

[Publications] J.Sugatani: "Occurrence of platalet-activating factor(PAF)in normal rat stomach and alteration of PAF level by water immersion stress" FASEB J.3. 65-70 (1989)
[Publications] K.Fujimura: "Serum platelet-activating factor acetylhyrolase activity in rats with gastric ulcers induced by water-immersion stress" Scand.J.Gastroenterol. 24(Suppl162). 59-62 (1989)
[Publications] R.Nakayama: "Presence of 1-0-alk-1'-enyl-2-0-acetyl glycerophosphocholine(vinyl form of PAF)in perfused rat and guinea pig hearts" J.Biochem.105. 494-496 (1989)
[Publications] Y.Tatsumi: "Changes in phospholipid composition of the mitochondrial membrane after orthotopic liver transplantation" Life Sci.44. 1053-1058 (1989)
[Publications] T.Okumura: "A sex difference in the effect of prostaglandins on hormonestimulated glycogenolysis in primary cultures of rat hepatocytes" Biochim.Biophys.Acta. 1051. 300-305 (1990)
[Publications] S.Kuma: "Effect of human recombinant interleukin-6 on the proliferation of mouse hepatocytes in the primary culture" Immunobiolgy. (1990)
[Publications] T.Horii: "Platelet-activating factor detected in bronchoalveolar lavage fluids from a patient with bronchial asthma" Lipids. (1990)
[Publications] J.Sugatani: "Molecular heterogeneity of platelet-activating factor(PAF)in normal rat glandular stomach determined by gas chromatography/mass spectrometry:alteration in molecular species of PAF by water-immersion stress" Lipids. (1990)
[Publications] J.Sugatani: "Development of a novel scintillation proximity radioimmunoassay for platelet-activating factor measurement:cmparison with say bioassay and GC/MS techniques" Life Sciences. (1990)
[Publications] M.Oda: "The presence of platelet-activating factor associated with eosinophil and/or neutrophil accumulations in the pleural fluids" Am.Rev.Respir.Dis.(1990)
[Publications] M.Furukawa: "Platelet-activating factor detected in nasal polyps" Acta Otolaryngol(Stock). (1990)
[Publications] T.Morimoto: "Monosodium urate crystals(MSUC)stimulate PAF production by human mononuclear leukocytes" FEBS Letters. (1990)
[Publications] K.Saito: "Three forms of PAF in normal rat tissues" "Platelet-Activating Factor and Diseases",ed.by Kunihiko Saito and Donald J.Hanahan. 19-35 (1989)
[Publications] K.Satouchi: "The discovery and present status of platelet-activating factor" "PAF in asthma",ed.by G.Holme and J.Morley. 3-19 (1989)
[Publications] K.Saito: "PAF in normal rat uterine tissue" "PAF in Asthma",ed.by G.Holme and J.Morley. 97-102 (1989)
[Publications] K.Saito: "PAF analogues in normal rat tissues" Proceedings of the Second International Symposium on Mass Spectrometry in the Health and Life Scieneces. (1990)
[Publications] K.Saito: "Phospholipase B in Penicillium notatum" "Phospholipases",ed.by E.Dennis,a volume of Methods in Enzymology,ed.by J.N.Abelson and M.I.Simon. (1990)
[Publications] Ed.by Kunihiko Saito and Donald J.Hanahan: ""Platelet-Activating Factor and Diseases"" International Medical Publishers,Ltd., 206 (1990)

1989 Fiscal Year Annual Research Report

正常組織におけるPAFとその内在性インヒビタ-

Principal Investigator

斎藤 國彦 関西医科大学, 医学部, 教授 (80077560)

Research Products

[Publications] J.Sugatani: "Occurrence of platalet-activating factor(PAF)in normal rat stomach and alteration of PAF level by water immersion stress" FASEB J.3. 65-70 (1989)

[Publications] K.Fujimura: "Serum platelet-activating factor acetylhyrolase activity in rats with gastric ulcers induced by water-immersion stress" Scand.J.Gastroenterol. 24(Suppl162). 59-62 (1989)

[Publications] R.Nakayama: "Presence of 1-0-alk-1'-enyl-2-0-acetyl glycerophosphocholine(vinyl form of PAF)in perfused rat and guinea pig hearts" J.Biochem.105. 494-496 (1989)

[Publications] Y.Tatsumi: "Changes in phospholipid composition of the mitochondrial membrane after orthotopic liver transplantation" Life Sci.44. 1053-1058 (1989)

[Publications] T.Okumura: "A sex difference in the effect of prostaglandins on hormonestimulated glycogenolysis in primary cultures of rat hepatocytes" Biochim.Biophys.Acta. 1051. 300-305 (1990)

[Publications] S.Kuma: "Effect of human recombinant interleukin-6 on the proliferation of mouse hepatocytes in the primary culture" Immunobiolgy. (1990)

[Publications] T.Horii: "Platelet-activating factor detected in bronchoalveolar lavage fluids from a patient with bronchial asthma" Lipids. (1990)

[Publications] J.Sugatani: "Molecular heterogeneity of platelet-activating factor(PAF)in normal rat glandular stomach determined by gas chromatography/mass spectrometry:alteration in molecular species of PAF by water-immersion stress" Lipids. (1990)

[Publications] J.Sugatani: "Development of a novel scintillation proximity radioimmunoassay for platelet-activating factor measurement:cmparison with say bioassay and GC/MS techniques" Life Sciences. (1990)

[Publications] M.Oda: "The presence of platelet-activating factor associated with eosinophil and/or neutrophil accumulations in the pleural fluids" Am.Rev.Respir.Dis.(1990)

[Publications] M.Furukawa: "Platelet-activating factor detected in nasal polyps" Acta Otolaryngol(Stock). (1990)

[Publications] T.Morimoto: "Monosodium urate crystals(MSUC)stimulate PAF production by human mononuclear leukocytes" FEBS Letters. (1990)

[Publications] K.Saito: "Three forms of PAF in normal rat tissues" "Platelet-Activating Factor and Diseases",ed.by Kunihiko Saito and Donald J.Hanahan. 19-35 (1989)

[Publications] K.Satouchi: "The discovery and present status of platelet-activating factor" "PAF in asthma",ed.by G.Holme and J.Morley. 3-19 (1989)

[Publications] K.Saito: "PAF in normal rat uterine tissue" "PAF in Asthma",ed.by G.Holme and J.Morley. 97-102 (1989)

[Publications] K.Saito: "PAF analogues in normal rat tissues" Proceedings of the Second International Symposium on Mass Spectrometry in the Health and Life Scieneces. (1990)

[Publications] K.Saito: "Phospholipase B in Penicillium notatum" "Phospholipases",ed.by E.Dennis,a volume of Methods in Enzymology,ed.by J.N.Abelson and M.I.Simon. (1990)

[Publications] Ed.by Kunihiko Saito and Donald J.Hanahan: ""Platelet-Activating Factor and Diseases"" International Medical Publishers,Ltd., 206 (1990)

斎藤國彦関西医科大学, 医学部, 教授 (80077560)