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1990 Fiscal Year Final Research Report Summary

PAF in Normal Rat Tissues and its Endogenous Inhibitors

Research Project

Project/Area Number 63480132
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionKansai Medical University

Principal Investigator

SAITO Kunihiko  Knasai Medical University, Department of Medical Chemistry Professor, 医学部, 教授 (80077560)

Co-Investigator(Kenkyū-buntansha) SUGATANI Junko  Kansai Medical University, Department of Medical Chemistry Instructor (from Apri, 医学部, 講師 (30098131)
NAKAYAMA Reiko  Kansai Medical University, Department of Medical Chemistry Assistant (Moved to K, 食物学科, 講師 (60172483)
SATOUCHI Kiyoshi  Kansai Medical University, Department of Medical Chemistry Instructor (Moved to, 工学部, 助教授 (50098125)
Project Period (FY) 1988 – 1990
KeywordsPAF / Endogenous PAF inhibitors / Vinyl PAF / PGF<@22alpha@>2 / Rat / Stomach / Heart muscle / Ovary
Research Abstract

The evidence we obtained indicated that PAF (1-alkyl-2-acetyl-sn-glycero-3-phosphocholine, alkylacetyl GPC) has a biphasic biological meaning : one is an allergic and inflammatory mediatory as well recognized and the other is an autacoid in normal biophysiology. It should be also emphasized taht PAF is the first example of a biologically active natural phospholipid. In addition to PAF, acylPAF (acylacetyl GPC) and vinylPAF (alk-1^'-enylacetyl GPC), both of which are PAF analogues, are also present in normal tissues but their biomedical meaning is still unknown.
Our main object of this research has been focussed on PAF of autacoid, and the results we obtained are as follows :
1. PAF level of normal rat stomach
The PAF levels of forstomach, corpus, antrum and duodenum are different and antrum is highest, 940+200 nmol/mol tissue phospholipid, and forstomach is lowest. The biological meaning of the endogenous PAF in normal tissue is uncertain but when a rat is immersed in water at 23 ^゚C for severalhr, the PAF level in antrum decreased rapidly to approximately 40 % of the original value at 1 hr, when the ulcer formation by the stress is scarcely recognized. The typical ulcer develops at 7 hr.
2. VinylPAF in normal rat heart
We reported for the first time the presence of vinylPAF in normal rat and guinea pig hearts. The platelet aggregating activity is 20 % of the corresponding PAF.
3. Effects of estradiol on PAF and PG levels of oophorectomized rat uterus
By oophorectomy, the PAF level of the uterus decreased and by estradiol administration its level recovers. At that time, PGF_<2alpha> increases in parallel with PAF but PGE_2 level slightly decreases.
From these evidence PAF and the analogues are present in normal rat tissues and the level is changed in response to various non-inflammatory stmuli, suggesting that the PAF and its analogues, vinylPAF at least, play a important role as an autacoid.
Further development in this line will be expected in future.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Reiko Nakayama: "1ー0ーhexadecー1'ーenylー2ーacetylーsnーglyceroー3ーphosphocholine and its biological activity" Biochem.Biophys.Res.Commun.151. 1256-1261 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katsuhiko Yasuda: "Acyl type plateletーactivating factor in normal rat uterus determined by gas chromatography mass spectrometry" Biomed.Environ.Mass Spectrom.16. 137-141 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Junko Sugatani: "Occurrence of plateletーactivating factor(PAF)in normal rat stomach and alteration of PAF level by water immersion stress" FASEB J.3. 65-70 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Reiko Nakayama: "Presence of 1ー0ーalkー1'ーenylー2ー0ーacetyl glycerophosphocholine(vinyl form of PAF)in perfused rat and guinea pig hearts" J.Biochem.105. 494-496 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Junko Sugatani: "Development of a novel scintillation proximity radioimmunoassay for plateletーactivating factor measurement:comparison with bioassay and GC/MS techniques" Life Sciences. 46. 1443-1450 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Reiko Nakayama: "Effect of 17βーestradiol on PAF and prostaglandin levels in oophorectomized rat uterus" Biochim.Biophys.Acta.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kunihiko Saito and Donald J.Hanahan: "PlateletーActivating Factor and Diseaes" International Medical Publishers,Tokyo, 206 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kunihiko Saito: "PAF analogues in normal rat tissues,in "Biological Mass Spectrometry",ed.by A.L.Burlingame and J.A.McCloskey" Elsevier,Amsterdam, 527-547 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Reiko Nakayama: "1-0-hexadec-1'-eny1-2-acetyl-sn-glycero-3-phosphocholine and its biological activity" Biochem. Biophys. Res. Commun.151. 137-141 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katsuhiko Yasuda: "Acyl type platelet-activating factor in normal rat uterus determined by gas chromatography mass spectrometry" Biomed. Env. Mass Spectrom.16. 137-141 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Junko Sugatani: "Occurrence of platelet-activating factor (PAF) in normal rat stomach and alteration of PAF level by water immersion stress" FASEB J.3. 65-70 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Reiko Nakayama: "Presence of 1-0-alk-1^'-eny1-2-0-acetyl glycerophosphocholine (vinyl form of PAF) in perfused rat and guinea pig hearts" J. Biochem. 105. 494-496 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Junko Sugatani: "Development of a novel scintillation proximity radioimmunoassay for platelet-activating factor measurement : Comparison with bioassay and GC/MS techniques" Life Sciences. 46. 1443-1450 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Reiko Nakayama: "Effect of 17beta-estradiol on PAF and prostaglandin levels in oophorectomized rat uterus" Biochem. Biophys. Acta.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kunihiko Saito and Donald J. Hanahan (Eds.): International Medical Publishers, Tokyo. Platelet-Activating Factor and diseases, 1989 (206)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kunihiko Saito: Elsevier, Amsterdam. PAF analogues in normal rat tissues, in "Biological Mass Spectrometry", ed. by A. L. Burlingame and J. A. McCloskey, 1990 (527-)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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