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1989 Fiscal Year Final Research Report Summary

Regulation of Gene Expression by (ADP-ribosyl)ation Reaction

Research Project

Project/Area Number 63480136
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Pathological medical chemistry
Research InstitutionKochi Medical School

Principal Investigator

SHIZUTA Yutaka  Kochi Medical School, Professor. -> 高知医科大学, 医学部, 教授 (50025631)

Co-Investigator(Kenkyū-buntansha) TODA Katsumi  Kochi Medical School, Associate Professor., 医学部, 助教授 (40197893)
Project Period (FY) 1988 – 1989
KeywordsADP-ribosylation / cDNA cloning / Genomic DNA cloning / Monoclonal Antibody / Microinjection
Research Abstract

ADP-ribose transferase plays an important role in gene expression of eukaryotic cells. In the present study, we have isolated CDNA as well as genomic DNA for the enzyme and elucidated the characteristics of the gene as follows.
1) The open reading frame of the cDNA encodes a protein of 1,0141 amino acid residues with a molecular weight of 113,153. The DNA binding domain involves a homologus repeat in the sequence and exhibits a sequence homology with localized region of transforming proteins such as c-fos and v-fos.
2) The promoter region of the transferase gene lacks TATA box and CAT box, but it contains two potent Sp1 binding sites and three putative AP-2 binding elements. Northern blot analysis has revealed that transcription of the transferase gene is markedly stimulated both by cyclic AMP and by phorbol ester.
3) Using domain-specific nionoclonal antibodies, the half life of the transferase in the living cells was determiner to be approximately 15 hours. The antibodies appear to inhibit thymidine uncorporation into living cells, suggesting that the enzyme also plays an important role in DNA replication.

Research Products

(14 results)

All Other

All Publications

  • [Publications] K.Kurosaki et al.: "Primary Structure of Human Poly(ADP-ribose)Synthetase as Deduced by cDNA Sequence." J.Boil.Chem.263. 7437-7438 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Yokoyama et al.: "Human Poly(ADP-ribose)Synthetase Gene." J.Biol.Chem.265. (inpress)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Toda et al.: "Alternative Usage of Different Poly(A)Addition Signals for Two Major Species of mRNA Encoding Human Aromatase P-450." FEBS Letters.247. 371-376 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Mitsuuchi et al.: "Cloning and Expression of cDNAs for Human Poly(ADP-ribose)Synthetase." Eur.J.Biochem.(in preparation). 189.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 戸田勝己,他: "ヒト胎盤アロマタ-ゼP-450のcDNAクロ-ニング" 生化学. 61. 524 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 戸田勝己,他: "ヒト胎盤アロマタ-ゼのcDNAの単離とその発現機構" 生化学. 61. 852 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Shizuta et al.: "(Molecular Structure of Poly(ADP-ribose)Synthetase)ADP-Ribose Transfer Reactions." Springer-Verlag, 527 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] I.Kameshita et al.: "(Synthesis and Degradation of Poly(ADP-ribose)Synthetase)ADP-ribose Transfer Reactions." Springer-Verlag, 527 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K. Kurosaki et al.: "Primary Structure of Human Poly(ADP-ribose) Synthetase as Deduced by cDNA Sequence." J. Biol. Chem. 262 7437-7438, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Yokoyama et al.: "Human Poly(ADP-ribose) Synthetase Gene." J. Biol. Chem. 265.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Toda et al.: "Alternative Usage of Different Poly(A) Addition Signals for Two Major Species of mRNA encoding Human Aromatse P-450." FEBS Letters 247 371-376, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Mitsuuchi et al.: "Cloning and Expression of cDNAs for Human Poly(ADP-ribose) Synthetase." Eur. J. Biochem. 189.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Shizuta et al.: "(Molecular Structure of Poly(ADP-ribose) Synthetase) ADP-Ribose Transfer Reactions" Springer-Verlag 527, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] I. Kameshita et al.: "(Synthesis and Degradation of Poly(ADP-ribose) Synthetase) ADP-ribose Transfer Reactions" Springer-Verlag 527, 1989.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-25  

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