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1990 Fiscal Year Final Research Report Summary

Mechanism of Protection of Cells from Damage of Autologous Complement

Research Project

Project/Area Number 63480165
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Immunology
Research InstitutionUniversity of Tsukuba

Principal Investigator

TAMUTA Nioboru  Univ. of Tsukuba, Inst. Basic Med. Sci. Prof., 基礎医学系, 教授 (90076977)

Co-Investigator(Kenkyū-buntansha) FUJITA Teizo  Univ. of Tsukuba, Inst. Basic. Sci., Associate Prof., 基礎医学系, 助教授 (20134223)
Project Period (FY) 1988 – 1990
KeywordsComplement Regulatory Protein / C3 Convertase / DAF / C4NeF / C3NeF / C8 / C9 / Homologous Restriction Factor
Research Abstract

We showed in the study ; (1) decay- accelerating factor (DAF) acts as a regulatory protein by dissociating C2a or Bb from classical and alternative C3 convertases, (2) DAF on the nucleated cells exhibits the same molecular weight and activity as DAF on red cells, (3) incorporation of DAF into red cells from paroxysmal nocturnal hemoglobinuria prolongs life-span of the cells, (4) DAF functions to inhibit the assembly of C3 convertases even in the presence of C4 or C3 nephritic factor which is autoantibody against classical or alternative C3 convertase to stabilize the convertase.
Concerning a regulatory protein affecting on the late-acting components of the complement we found ; (1) it protects neuraminidase-treated human red cells from hemolysis by human complement via alternative pathway, (2) its molecular weight is 20 kDa, (3) incorpo-ration of the purified factor into guinea pig red cells renders the cells resistant to human complement attack by both classical and alternative pathways, (4) it interferes with the action of human C8 and C9, but not with that of rabbit C8 and C9. These results indicate that the factor is a homologous restriction factor.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Fujita,T.: "Purification and characterization of decayーacceleーrating factor (DAF) from Raji cells." Immunology. 64. 369-374 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ninomiya,H.: "Decayーaccelerating factor (DAF) on the blood cell membranes in patients with paroxysmal nocturnal haemoglobinuria (PNH)." British J.Haematology. 69. 81-87 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ito,S.: "Effect of decayーaccelerating factor on the assembly of the classical and alternative pathway C3 convertases in the presence of C4 or C3 nephritic factor." Immunology. 68. 449-452 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okada,N.: "A novel membrane glycoprotein capable of inhibiting membrane attack by homologous complement." International Immunology. 1. 205-208 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okada,N.: "Monoclonal antibodies capable of causing hemolysis of neuraminidaseーtreated human erythrocytes by homologous complement." J.Immunology. 143. 2262-2266 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ninomiya,H.: "Prolongation of redーcell lifeーspan by incorporation of decayーaccelerating factor (DAF) into red cells of paroxysmal nocturnal haemoglobinuria (PNH)." British J.Haematology. 73. 132-133 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimosato,T.: "Suppression of tumor necrosis alpha production by a human immunoglobulin preparation for itravenous use." Infection&Immunity. 58. 1384-1390 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Harada,R.: "Purification of 1F5 antigen that prevents complement attack on homologous cell membranes." J.Immunolology. 144. 1823-1828 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujita, T. et al. :"Purification and characterization of decay-accelerating factor (DAF) from Raji cells." Immunology. 64. 369-374 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ninomiya, H. et al. :"Decay-accelerating factor (DAF) on the blood cell membranes in patients with paroxysmal nocturnal hamoglobinuria (PNH)." Brit. J. Haematol.69. 81-87 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito, S. et al. :"Effect of decay-accelerating factor on the assembly of the classical and alternative pathway C3 convertases in the presence of C4 or C3 nephritic factor." Immunology. 68. 449-452 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okada, N. et al. :"A novel membrane glycoprotein capable of inhibiting membrane attack by homologous complement." Intern. Immunol.1. 205-208 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okada, N. et al. :"Monoclonal antibodies capable of causing hemolysis of neuraminidase-treated human erythrocytes by homologous complement." J. Immunol.143. 2262-2266 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ninomiya, H. et al. :"Prolongation of red-cell life- span by incorporation of decay-accelerating factor (DAF) into red cells of paroxysmal nocturnal haemoglobinuria (PNH)." Brit. J.. Haematol.73. 132-133 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimosato, T. et al. :"Suppression of tumor necrosis factor alpha production by a human immunoglobulin preparation for intravenous use." Infect. Immun.58. 1384-1390 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Harada, R. et al. :"Purification of 1F5 antigen that prevents homplement attack on nomologous cell membranes." J. Immunol.144. 1823-1828 (1990)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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