Molecular mechanism of the abnormal expression of MHC-linked complement genes.

1989 Fiscal Year Final Research Report Summary

• Project/Area Number: 63480167
• Research Category: Grant-in-Aid for General Scientific Research (B)
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: Cancer Research Institute, Kanazawa University
• Principal Investigator: TAKAHASHI Morinobu CANCER RESEARCH INSTITUTE DEPARTMENT OF IMMUNOBIOLOGY, PROFESSOR., がん研究所, 教授 (80019877)
• Project Period (FY): 1988 – 1989
• Keywords: Transcriptional regulation / Complement C4 / Slp gene / Complement deficiency / Transciptional factor / トランススペライシング

Research Abstract

Regulatory mechanism underlying the abnormal expression of complement C4 and Slp genes of mouse was investigated at molecular level, using a wide variety of techniques of molecular biology. A few very important findings have been obtained from the present work which are summarized as follows.
I.Post transckiptional and pretranslational regulation was shown to determine low C4 expression of C4 gene of H-2^k mouse(BlO.BR). C4 cDNA was isolated from the liver cDNA library which contains sequences probably derived by transsplicing mechanism.
The transcriptional regulatory domains were identified in the 5' region of mouse C4 and Slp genes. The recognition mothif homologous to NF.kB/H2TF^1_ was identified in the region of C4 gene but not of Slp gene. Nuclear factors of mouse liver binds to this recognition mothif of C4 gene and probably enhance the transciption of C4 gene.

Research Products

• [Publications] Yu,D.Y.,Huang,Z.M.,Murakami,S.,Takahashi,M.& Nonaka,M.: "Specific binding of a hepatoma nuclear factor to the NF.kB/H2TF1 recognition motif found in the C4 promoter,but not in the SLp promoter." Journal of Immunology. 143. 2395-2400 (1989)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nakayama,K.,Pattanakitsakul,S.,Yokoyama,S.……Takahashi,M.: "Posttranscriptional regnlation of complement C4 in low C4 producing strain of mouse." Immunogenetics(印刷中). (1990)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sa-ngu,Pattanakitsakul,Nakayama,K,.Nonaka,M.& Takahashi,M.: "Occurrence of aberrantly spliced mRNA associated with complement C4 gene expression of low C4 producing strain of mouse" Proc,Natl.Acad.Sci,USA(提出中).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sa-ngu,P.,Nakayama,K.,Nonaka,M,.& Takahashi,M.: "Multiple recombination occurring in the he hotspot of MHC class III genes results in the C4-Slp recombinant gones with altered gene expression." Immunogenetics(提出中).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yu, D. Y., Nonaka, M. & Takahashi, M: "Mapping of the transcriptional regulatory domains for the difference in the promoter activity between mouse C4 and Slp(sex limited protein) genes." Journal of Immunology 141, 4381-4387, 1988.
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yu, D. Y., Huang, Z.M., Murakami, S., Takahashi, M., & Nonaka, M: "Specific binding of a hepatoma nuclear factor to the NF. kappaB/H2TF1 recognition motif found in the C4 promoter, but not in the Slp promoter." Journal of Immunology 143, 2395-2400, 1989.
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nakayama, K., Sa-nga, P., Yokoyama, S., Kimura, H., Nonaka, M. & Takahashi, M.: "Posttranscriptional regulation of complement C4 in low C4 producing strain of mouse." Immunogenetics 1990.
  • Description: 「研究成果報告書概要(欧文)」より