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1990 Fiscal Year Final Research Report Summary

Effects of Neurotransmitters on Acetylcholine Release in the Brain-In Vivo Dealysis

Research Project

Project/Area Number 63480192
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionHiroshima University

Principal Investigator

KATAYAMA Sadao  Hiroshima Univ. School of Medicine, Instructor, 医学部・附属病院, 助手 (00211160)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Shigenobu  Hiroshima Univ. School of Medicine, professor, 医学部, 教授 (30026843)
KITO Syozo  Univ. of the Air Fuculty of Liberal Acts, professor, 教養学部, 教授 (00010140)
Project Period (FY) 1988 – 1990
KeywordsMammalian brain / Acetylcholine release / In vivo intracerebral dialysis / Neuropeptide / Neurotransmitter interactions
Research Abstract

Acetylocholine (ACh) has been considered to be involved in the higher brain function such as learning and memory in the cerebral cortex and hippocampus, while in the basal ganglia and cerebellum it has relations with movement. It is important to elucidate the turnover of ACh in the brain not only inthe basic neuroscience but also for understanding of pathophysilogy and treatment of neurodegenerative disorders such as Alzheimer's disease. In this research project, we established the method for measuring ACh contents from in vivo dialysis samples of the brain. By use of method, the effects of various neurotransmitters and drugs on the release of ACh were studied in the rat brain.
First, we estabilished the method for measuring the endogenous ACh release in the striatum and hippocampus. The dialysis probe which was equipped with a semipermeable tube, was inserted into the rat brain. On perfusion experiments, rats were allowed to move freely and perfused with a Ringer solution. The perfusat … More es were collected with 20-min intervals and, ACh contents were measured by HPLC. When perfused with a Ringer solution, ACh contents were very low and not detectable. On the other hand, they were able to be detected in the presence of an anticholinesterase, physostigmine. Thus, we confirmed that ACh in the perfusates was released from cholinergic nerve terminals. As the next step, effects of neuropeptides, cholinergic drugs and nerve growth factor on ACh release were studied. Peripheraly administration of cholecystokinin induced an increase of ACh release in the striatum, and the results suggested that the action of the peptide was, in part, mediated via vagal afferent impulses. Neurotensin did not affect the striatal cholinergic release, while it increased the release of dopamine and its metabolites. A muscarinic ACh receptor agonist inhibited the release of ACh in the striatum, whereas an antagonist stimulated it. In addition, LH-RH had no effect on the hippocampal ACh release, while it decreased the release of glutamate.
In summary, the mechanisms of ACh release and its modulation by other neuroactive substances were investigated in the rat brain. Since the experimental system is in vivo, these results have possibilities for application to drug development. Less

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Shimoyama,M.: "Effect of cerulein on in vivo release of acetylcholine from the rat striatum" Brain Research. 492. 381-384 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyoshi,R.: "Quantitative autoradiographic localization of the M_1 and M_2 subtypes of muscarinic acetylcholine receptors in the monkey brain" Japanese Journal of Pharmacology. 51. 247-255 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyoshi,R.: "Modulation of dopamine D1 receptor binding by neurotensin in the rat striatum" Neurochemistry Internatioal. 15. 493-496 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamura,S.: "Subcelular distribution of acetylcholinesterase in Alzheimer's disease:abnormal localization and solubilization" J.Neural Transm.30. 13-23 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamura,S.: "Senile dementia and presenile dementia" Tohoku J.Exp.Med.161. 49-60 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimoyama,M.: "Effect of neurotensin on in vivo release of dopamine from the rat striatum" EUR.J.Pharmacology. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyoshi,R.: "Basal Ganglia III (Effect of neurotensin on dopamine and muscarinic acetylcholine receptors in the rat brain)" Plenum Press, IN TRESS (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kito,S.: "Neurotransmitter Receptors: Neuroreceptor Mechanisms in Brain (Effect of Neuropeptides on Clasic Types of Neurotransmission in the Rat Central Nervous System)" Plenum Press, 11 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimoyama, M.: "Effect of cerulein on in vivo release of acetylcholine from the rat striatum" Brain Research. vol. 492. 381-384 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyoshi, R.: "Quantitative autoradiographic localization of the M_2 and M_2 subtypers of muscarinic acetylcholine receptors in the monkey brain" Japanese Journal of Pharmacology. vol. 51. 247-255 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyoshi, R.: "Modulation of dopamine Dl receptor binding by neurotensin in the rat striatum" Neurochemisyry International. vol. 15. 493-496 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamnra, S.: "Subcelular distribution of acetylcholinesterase in Alzheimer's disease : abnormal localization and solubilization" J. Neural Transm. vol. 30. 13-23 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura, S.: "Senile dementia and presenile dementia" Tohoku J. Exp. Med.vol. 161. 49-60 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimoyama, M.: "Effect of neurotensin on in vivo release of dopamine from the rat striatum" EUR. J. Pharmacology. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyoshi, R. III: Plenum Press. Basal Ganglia (Effect of neurotensin on dopamine and muscarinic acetylcholine receptors in the rat brain), (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kito, S.: Plenum Press. Neurotransmitter Receptors : Neuroreceptor Mechanisms in Brain (Effect of Neuropeptides on Classic Types of Neurotransmission in the Rat central Nervous System), 11 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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