1990 Fiscal Year Final Research Report Summary
The Mechanism of Spontaneous Maturation in Human Neuroblastoma
| Project/Area Number |
63480238
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Pediatrics
|
|---|
| Research Institution | Kyoto Prefectural University of Medicine |
|---|
Principal Investigator |
SAWADA Tadashi Kyoto Prefectural University of Medicine Department of Medicine, Professor, 医学部, 教授 (10079874)
|
|---|
| Project Period (FY) |
1988 – 1990
|
| Keywords | Neuroblastoma / Spontaneous maturation / Differentiation / N-myc / c-src / HLA Class PI / HLAclassII抗原 |
|---|
| Research Abstract |
Neuroblastoma (NB), a malignant tumor that arises from the neural crest, is well known for its unique potential for spontaneous maturation in vivo and its capacity to differentiate in vitro. Proto-oncogenes comprise a class of cellular genes that are thought to be of key importance in the control of cell proliferation and differentiation.
1. N-myc oncogene was often amplified and overexpressed in human NB cell lines and stage-advanced NB tumor samples.
2. Decreased N-myc expression and increased c-arc expression were observed during neuronal differentiation by retinoic acid, polyprenoic acid (E5166) and dibutyryl cyclic AMP, whereas the expression of N-myc and cーsrc was reduced during schwannian differentiation by bromodeoxyuridine.
3. Induction of morphological differentiation and HLA class II antigens (HLA-DR and -DP) was observed by gamma -interferon.
These results suggest that enhanced N-myc expression might be closely linked to the undifferentiated phenotypes of NB cells, that N-myc and c-src genes might be regulated independently in the determination of the bipolar differentiation of NB cells, and that HLA class II expression by gamma -interfeon might be related to the differentiation stage of NB cells.
|
