1989 Fiscal Year Final Research Report Summary
Functional activation of human monocytes by recombinant human macrophage colony-stimulating factor.
| Project/Area Number |
63480279
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Jichi Medical School |
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Principal Investigator |
MOTOYOSHI Kazuo Jichi Med. Sch. Dept. of Med. Assoc. Prof., 医学部, 助教授 (80137702)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Masaki Jichi Med. Sch. Dept. of Med. Prof., 医学部, 教授 (60012762)
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| Project Period (FY) |
1988 – 1989
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| Keywords | M-CSF / Granulocytopenia / Thrombocytopenia / Bone Marrow Transplantation / Survival rate / Megakaryocyte-potentiator / Tumor-killing activity / Myelodysplastic syndrome |
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| Research Abstract |
Following results were obtained in this research project.
1) When human macrophage colony-stimulating factor (hM-CSF) was administered after anticancer chemotherapy, it accelerated both granulocyte and platelet recovery in patients with gynecologic malignancy and granulocyte recovery in patients with hematological malignancy.
2) Human M-CSF accelerated the granulocyte recovery and increased the survival rate of patients within 4 months after bone marrow transplantation. Human M-CSF did not increase the rate of leukemic relapse at 12-27 months after bone marrow transplantation.
3) Human M-CSF activated human monocutes to produce megakaryocyte- potentiating factor (Meg-POT) in vitro.
4) Human M-CSF enhanced antibody-dependent tumor-killing activity of human monocutes to Raji cells (Burkitt lymphoma-derived cell line).
5) Human M-CSF enhanced antibody-independent tumor-killing activity of human monocutes to several human leukemic cell lines such as K562, HL60, U937 and Daudi.
6) Changes in serum human M-CSF levels were monitored during neutropenic period after anticancer chemotherapy using enzyme-linked immunosorbent assay (ELISA). Negative correlation was found between the serum M-CSF level during neutropenic period and the duration of neutropenia.
7) Injection of human M-CSF resulted in the reduction of serum total cholesterol level in human and rabbits.
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[Publications] Masaoka T, Motoyoshi K, Takaku F, Kato S, Harada M, Kanamaru A, Moriyama Y, Ohno R, Ohira M, Shibata H, Inoue T: "Administration of human urinary colony-stimulating factor after bone marrow transplantation." Bone Marrow Transplantation 3:121-127, 1988.
Description
「研究成果報告書概要(欧文)」より
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[Publications] Motoyoshi K, Yoshida K, Hatake K, Saito M, Miura Y, Yanai N, Yamada M, Kawashima T, Wong GG, Temple PA, Leary AC, Witek-Giannoti JS, Fujisawa M, Yuo A, Okabe T, Takaku F: "Recombinant and native human urinary colony stimulating factor directly augments granulocytic and granulocyte-macrophage colony-stimulating factor production of human peripheral blood monocytes." Exp Hematol 17:68-71, 1989.
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yanai N, Yamada M, Motoyoshi K, Yokota H, Yoshida K, Saito M, Kawashima T, Nishida M, Miura Y, Saito M, Takaku F: "Effect of human macrophage colony-stimulating factor on granulopoiesis and survival in bone marrow transplanted mice." Jpn J Cancer Res 81.
Description
「研究成果報告書概要(欧文)」より
