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1989 Fiscal Year Final Research Report Summary

Functional activation of human monocytes by recombinant human macrophage colony-stimulating factor.

Research Project

Project/Area Number 63480279
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Hematology
Research InstitutionJichi Medical School

Principal Investigator

MOTOYOSHI Kazuo  Jichi Med. Sch. Dept. of Med. Assoc. Prof., 医学部, 助教授 (80137702)

Co-Investigator(Kenkyū-buntansha) SAITO Masaki  Jichi Med. Sch. Dept. of Med. Prof., 医学部, 教授 (60012762)
Project Period (FY) 1988 – 1989
KeywordsM-CSF / Granulocytopenia / Thrombocytopenia / Bone Marrow Transplantation / Survival rate / Megakaryocyte-potentiator / Tumor-killing activity / Myelodysplastic syndrome
Research Abstract

Following results were obtained in this research project.
1) When human macrophage colony-stimulating factor (hM-CSF) was administered after anticancer chemotherapy, it accelerated both granulocyte and platelet recovery in patients with gynecologic malignancy and granulocyte recovery in patients with hematological malignancy.
2) Human M-CSF accelerated the granulocyte recovery and increased the survival rate of patients within 4 months after bone marrow transplantation. Human M-CSF did not increase the rate of leukemic relapse at 12-27 months after bone marrow transplantation.
3) Human M-CSF activated human monocutes to produce megakaryocyte- potentiating factor (Meg-POT) in vitro.
4) Human M-CSF enhanced antibody-dependent tumor-killing activity of human monocutes to Raji cells (Burkitt lymphoma-derived cell line).
5) Human M-CSF enhanced antibody-independent tumor-killing activity of human monocutes to several human leukemic cell lines such as K562, HL60, U937 and Daudi.
6) Changes in serum human M-CSF levels were monitored during neutropenic period after anticancer chemotherapy using enzyme-linked immunosorbent assay (ELISA). Negative correlation was found between the serum M-CSF level during neutropenic period and the duration of neutropenia.
7) Injection of human M-CSF resulted in the reduction of serum total cholesterol level in human and rabbits.

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Masaoka T et al: "Administration of human urinary colony-stimulating factor after bone marrow transplantation." Bone Marrow Transplantation. 3. 121-127 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hanamura T et al: "Quantitation and identification of human monocytic colony-stimulating factor in human serum by enzyme-linked immunosorbent assay." Blood. 72. 886-892 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirai K et al: "Modulation of human basophil histamine release by hemopoietic growth factors." Journal of Immunology. 141. 3958-3964 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Motoyoshi K et al: "Recombinant and native human urinary colony-stimulating factor directly augments granulocytic and granulocyte-macrophage colony-stimulating factor production of human peripheral blood monocytes." Experimental Hematology. 17. 68-71 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Motoyoshi K et al: "Serum cholesterol-lowering activity of human monocytic colony-stimulating factor." Lancet. 11. 326-327 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzu S et al: "Enhancing effect of human monocytic colony-stimulating factor on monocyte tumoricidal activity." Cancer Research. 49. 5913-5917 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Motoyoshi K et al(Book Chapter): "Monokines and Other Non-lymphocytic Cytokines" edited by Oppenheim,Alan R. Liss,Inc.,pp301-306 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Motoyoshi K et al(book Chapter): "Recent Progress in Cytokine Research" edited by Yamamura Y,Medical View Co.,Ltd.,pp151-172 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masaoka T, Motoyoshi K, Takaku F, Kato S, Harada M, Kanamaru A, Moriyama Y, Ohno R, Ohira M, Shibata H, Inoue T: "Administration of human urinary colony-stimulating factor after bone marrow transplantation." Bone Marrow Transplantation 3:121-127, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hanamura T, Motoyoshi K, Yoshida K, Saito M, Miura Y, Kawashima T, Nishida M, Takaku F: "Quantitation and identification of human monocytic colony-stimulating factor in human serum by enzyme-linked immunosorbent assay." Blood 72:886-892, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Motoyoshi K, Yoshida K, Hatake K, Saito M, Miura Y, Yanai N, Yamada M, Kawashima T, Wong GG, Temple PA, Leary AC, Witek-Giannoti JS, Fujisawa M, Yuo A, Okabe T, Takaku F: "Recombinant and native human urinary colony stimulating factor directly augments granulocytic and granulocyte-macrophage colony-stimulating factor production of human peripheral blood monocytes." Exp Hematol 17:68-71, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Motoyoshi K, Takaku F: "Serum cholesterol-lowering activity of human monocytic colony-stimulating factor." Lancet ii:326-327, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzu S, Yokota H, Yamada M, Yanai N, Saito M, Kawashima T, Saito M, Takaku F, Motoyoshi K: "Enhancing effect of human monocytic colony-stimulating factor on monocyte tumoricidal activity." Cancer Research 49:5913-5917, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzu S, Yanai N, Saito M, Kawashima T, Kasahara T, Saito M, Takaku F, Motoyoshi K: "Enhancement of the antibody-dependent tumoricidal activity of human monocytes by human monocytic colony-stimulating factor." Jpn J Cancer Res 81:79-84, 1990.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yanai N, Yamada M, Motoyoshi K, Yokota H, Yoshida K, Saito M, Kawashima T, Nishida M, Miura Y, Saito M, Takaku F: "Effect of human macrophage colony-stimulating factor on granulopoiesis and survival in bone marrow transplanted mice." Jpn J Cancer Res 81.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-26  

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