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1989 Fiscal Year Final Research Report Summary

The study of corelationship between the level of immunosuppression and the cytokine levels in kidney transplant recipients.

Research Project

Project/Area Number 63480292
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General surgery
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

OKA Takahiro  Kyoto Prefectural University of Medicine The Second Department of Surgery Professor and Chairman of Surgery, 医学部, 教授 (60079837)

Co-Investigator(Kenkyū-buntansha) YOSHIMURA Norio  Kyoto Prefectural of University of Medicine, The Second Department of Surgery As, 医学部, 助手 (00191643)
Project Period (FY) 1988 – 1990
Keywordskidney transplantation / cytokine / IL-2 receptor / mRNA / Rejection
Research Abstract

The present study examined the effects of CsA administered with steroid in vivo on the capacity of kidney transplant recipient mononuclear cells to generate cytokines and their gene expression at the level messenger RNA(mRNA). Peipherel blood mononuclear cells(PBMC) from CsA-Prednisolon(Pred) treated recipients displayed 40-50% inhibition of IL-2 and 66% inhibition of gamma-IFN production compared with normal individuals. Azathioprine(AZ)-pred treated recipients displayed significantly less inhibition of IL-2 and gamma-INF generation than CsA treated patients. Macrophages(mphi) from CsA-Pred treated recipients also displayed 60.0% inhibition (5.l+O.7 U/ml, n=20, p<0.01) of IL-1 production compared with normal individuals (13.O+2.9U/ml, n=21). These result were confirmed by the experiments using CDNA probe for IL-2, gamma-IFN and IL-l(beta). High levels of IL-2 and gamma-IFN mRNA in PRA-stimulated PBL or IL-1(beta) mRNA in LPS-stimulated mphi were present in normal individuals, but not in CsA treated recipients as judged by hybridization. Moreover, the relationship between the onset of acute rejection crises and the expression of IL-2 or transferrin(TF) receptor on PBL from recipients was investigated.
The percentage of lymphocytes expressed IL-2 or transperrin receptor was increased at the onset of acute rejection crises, indicating that the measurement of IL-2 or TF receptor is good indicater for acute rejection crises in kidney transplant recipients.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Norio Yoshimura: "Cyclosporine effect on immunoregulatory cells in kidney transplant recipients:Suppression of gamma-interferon and interleukin production" Transplantation Proceedings. 20. 69-74 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 吉村了勇: "リンフォカインの腎移植における免疫学的モニタリングへの応用-特にインタ-ロイキン2(IL-2)を指標として" 今日の移植. 1. 148-152 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岡隆宏: "抗IL-2レセプタ-抗体による免疫抑制" 臨床免疫. 21. 859-867 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Norio Yoshimura: "Combination therapy of cyclosporine with steroid inhibits γ-interferon and interleukin-1 gene expression at the level of mRNA synthesis in vivo" Journal of Clinical Immunology. 9. 322-328 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 吉村了勇: "腎移植患者末梢血リンパ球におけるトランスフェリンおよび。IL-2レセプタ--免疫学的モニタリングとしての有用性" 日本外科学会雑誌. 90. 794 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 吉村了勇: "シクロスポリン使用腎移植患者末梢血リンパ球におけるインタ-ロイキン2(IL-2)レセプタ-遺伝子の発現抑制" 日本外科学会雑誌. 9. 956 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Norio Yoshimura: "Cyclosporine effect on immunoregulatory cells in kidney transplant recipients: Suppression of gamma-interferon and interleukin production." Transplantation Proceedings, 20, 69-74, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Norio Yoshimura: "Combination therapy of cyclosporine with steroid inhibits gamma-interferon and interleukin-1 gene expression at the level of mRNA synthesis in vivo." Journal of Clinical Immunology, 9, 322-328, 1989.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-26  

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