1989 Fiscal Year Final Research Report Summary
Study on tumor necrosis factor-producing cells induced by biological response modifiers.
| Project/Area Number |
63480303
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | OSAKA UNIVERSITY MEDICAL SCHOOL |
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Principal Investigator |
MORI Takesada OSAKA UNIVERSITY, MEDICAL SCHOOL, Professor, 医学部, 教授 (60028496)
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| Co-Investigator(Kenkyū-buntansha) |
MUROTANI Masahiro OSAKA UNIVERSITY, MEDICAL SCHOOL, Instructor, 医学部附属病院, 医員
MORIMOTO Hideki OSAKA UNIVERSITY, MEDICAL SCHOOL, Instructor, 医学部, 助手 (70220085)
SHIMANO Takashi OSAKA UNIVERSITY, MEDICAL SCHOOL, Associate Professor, 医学部, 講師 (80144476)
MONDEN Takushi OSAKA UNIVERSITY, MEDICAL SCHOOL, Instructor, 医学部, 助手 (20174477)
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| Project Period (FY) |
1988 – 1989
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| Keywords | Biological response modifiers / Tumor necrosis factor / Immunostain / In situ hybridization |
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| Research Abstract |
In vitro treatment of human peripheral blood mononulcar cells with OK-432 resulted in the rapid production or tumor necrosis factor (TNF), interlcukin-1alpha (IL-1alpha) and interlcukin-1beta (IL-1beta). The newly synthesized TNF, IL-1alpha and IL-1beta were identified by cnzyme immunoassay (EIA) and immunocytochemical staining. While the ability of OK-432 to induce to production of cytokines varied individually, IL-1alpha and TNF activities in supernatant reached maximal levels at 12 hours after OK-432 stimulation. In contrast, IL-1beta activity reached peak at 6 hours and then decreased gradually. Among the subjects examined, close correlations were observed between the value of TNF and IL-1alpha (r=0.863). TNF and IL-1beta (r=0.825), IL-1alpha and IL-1beta (r=0.986) at 24 hours after OK-432 stimulation, by rapid microwave fixation of stimulated mononuclear cells, cytoplasmic accumulation of OK-432, TNF, IL-Ualpha and IL-1beta was immunocytochemically demonstrated using specific antibodies. EIA of TNF and/or IL-1 in vitro will be useful for assessment of the potential ability of various biological response modifiers (BRMs) to induce the production of these cytokines, and immunostaining of the cells producing cytokines by microwave fixation technique will contribute to understand the mechanism of immuno responses.
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