1989 Fiscal Year Final Research Report Summary
Study of The Heterogeneity in Cancer by Monoclonal Antibodies
| Project/Area Number |
63480312
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Department of 2nd Surgery. Tokai University, School of Medicine |
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Principal Investigator |
NAKASAKI Hisao Surgery Tokai University school of Med., 医学部・外科, 講師 (10056145)
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| Co-Investigator(Kenkyū-buntansha) |
TOKUDA Yutaka Surgery Tokai University school of Med., 医学部・外科, 助手 (20163975)
MITOMI Toshio Surgery Tokai University school of Med., 医学部・外科, 教授 (20055809)
WATANABE Keiich Physiology Tokai University school of Med., 医学部・病理, 教授 (00055865)
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| Project Period (FY) |
1988 – 1989
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| Keywords | Monoclonal Antibody / Mosaicism / Heterogeneity |
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| Research Abstract |
Serial sections of a cancer tissue specimen were prepared with a combination of monoclonal antibodies of different kinds-SHI (IgG_3), FH4(IgG_3), FH_6(IgM), AH_6(IgM), and TKH_2(IgM)-and stain ed with an immunohistochemical method(ABC kit) to examine stained areas. Staining properties of individual monoclonal antibodies were recognized as differences in stained areas, being indicative of mosaicism. This finding is considered one mode of expression of the heterogeneity of cancer.
A positive result was obtained in 100% of the cancer tissue specimens when a combination of monoclonal antibodies was used. The technique by means of monoclonal antibodies has been thus found to be effective for the histological diagnosis of cancer. The suggestion has also been made that elucidating its heterogeneity aids in the selection of an appropriate anticancer drug.
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