• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1990 Fiscal Year Final Research Report Summary

Identification of a Chromosome Carrying a Putative Tumor Suppressor gene in Human Choriocarcinoma by Microcell-Mediated Chromosome Transfer.

Research Project

Project/Area Number 63480363
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionKyushu University (1989-1990)
Hokkaido University (1988)

Principal Investigator

WAKE Norio  Medical Institute of Bioregulation Kyushu Univ., 生体防御医学研究所, 教授 (50158606)

Co-Investigator(Kenkyū-buntansha) IMAMURA Toshiro  Medical Institute of Bioregulation Kyushu Univ., 生体防御医学研究所, 助手 (10221095)
MIYAMOTO Shingo  Medical Institute of Bioregulation Kyushu Univ., 生体防御医学研究所, 助手 (40209945)
KATO Hidenori  Medical Institute of Bioregulation Kyushu Univ., 生体防御医学研究所, 助手 (60214392)
IWANAGA Tomohisa  Medical Institute of Bioregulation Kyushu Univ., 生体防御医学研究所, 助手 (80213304)
Project Period (FY) 1988 – 1990
KeywordsHydatidiform mole / Choriocarcinoma / Cell fusion / Microcell fusion / Tumor / Suppressing gene / cDNA expression libraries specific to #7 / Gene cloning
Research Abstract

There are two main mechanisms of origin for complete hydatidiform mole ; a) fertilization of an empty egg by a haploid sperm followed by duplication and b) fertilization of such an egg by two haploid spermatozoa. It is widely accepted that of all forms of pregnancy that had to choriocarcinoma the risk associated with moles is by far the highest. Homozygous expression of a recessive mutation of moles has been assumed to associate with this propensity to malignancies.
Alterations of several tumor suppressor genes together with activation of oncogenes are assumed to be necessary for choriocarcinogenesis. Genetic characteristics shown in the moles suggest that the former alterations are especially important to explore the multistep conversions to malignancies. Thus, individual chromosomes derived from normal cells were introduced into choriocarcinoma cells via microcell fusion. Evaluation of tumorigenicity in microcell hybrids suggested that chromosome #7 carried a putative tumor suppressor gene for choriocarcinoma. The gene imprinting or recessive mutation mmy be responsible for the inactivation of such a gene. Being able to correspond with the high propensity to malignancy of moles. Now, the cDNA expression liberaties specific to the normal #7 chromosome could be produced. Colony hybridization using mRNA probes obtained from normal villi and CCI choriarcinoma in addition to suicide selection cloning the revertant will be able to clone the tumor suppressor genes of choriocarcinoma.

  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Shinkal,N: "Nonーsurgical MTX therapy for patients with tubalpregnancies,in preservation of tuboーovarlan function in gynecologic nenign and malignant diseases" Raven Press,PP. 595-603 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 藤本 征一郎: "尿路感染症,産婦人科領域感染症" 医薬ジャ-ナル社. 92-103 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安田 晶子: "加齢と胞状奇胎" 産婦人科の実際. 37. 125-128 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 新開 奈保子: "前期破水と子宮収縮のコントロ-ル" ペリタイタル・ケア. 7(6). 518-525 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 和気 徳夫: "II染色体分析技術の実際 D.腫瘍細胞の培養と染色体分析 8子宮内膜癌" 臨床病理. 80. 242-251 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 藤野 敬史: "DNA診断ー分子生物学の臨床応用 4.ヒト癌遺伝子研究の動向とDNA診断、(2)絨毛癌 胞状奇胎" 日本臨床. 589. 695-700 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 和気 徳夫: "羊水過多と羊水過少の次回妊娠に対する対応" 産婦人科の実際. 38. 383-388 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山田 秀人: "細胞融合法を用いた子宮内膜癌化機構解明へのアプロ-チ" 日本産科婦人科学会雑誌. 41. 122-128 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 涌井 之雄: "高度尿中蛋白漏出を肺水腫に至った重症妊娠中毒症の1症例" 周産期医学. 19. 134-138 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakai,K: "MTX resistant mechanims in human choriocarcinomg cells." Gynecologic Oncol. 34. 7-11 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 相原 稔彦: "部分奇胎の発生に関する細胞遺伝学的研究" 日本産科婦人科学会雑誌. 42. 67-72 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wake,N: "Isolation of clones resistant to 6ーthioguanine and G418 from the HHUA endometrial carcinoma cells and application to cell hybridization." Cancer Genet.Cytogenet. 49. 117-123 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamada,H: "Multiple chromo somes carring tumor suppressor activity fora uterine endometrial carcinoma cell line identifier by microcellーmediated chromosome transfer" Oncogene. 5. 1141-1147 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 和気 徳夫: "Flcorescent in Situ Hybridezstionについて" オンコロジア. 123(6). 141-142 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 和気 徳夫: "胞状奇胎からの絨毛癌化ーその機構と研究の現状ー" オンコロジア. 24. 31-39 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shinkai, N.: "Nonーsurgical MTX therapy for patients with tubal pregnancies. In preservation of tubo-ovarian function in gynecologic henign and malignant diseases." Raven Press, PP.595-603 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakai, K.: "MTX resistant mechanisms in human choriocarcinoma cells." Gynecologic Oncel.34. 7-11 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wake, N.: "Isolation of clones resistant to 6-thioguanine and G418 from the HHUA endometrial carcinoma cells and their application to cell hybridization." Cancer Genet. Cytogenet. 49. 117-123 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada, H.: "Multiple chromosomes carrying tumor suppressor activity for a uterine endometrial carcinoma cell line identified by microcell - mediated chromosome transfer." Oncogene. 5. 1141-1147 (1990)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1993-08-12  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi