1989 Fiscal Year Final Research Report Summary
The immunological investigations concerning the pathological mechanisms of periodontal diseases
| Project/Area Number |
63480420
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
ISHIKAWA Isao Tokyo Medical and Dental University Dental School Professor, 歯学部, 教授 (10014151)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEFUTA Wataru Tokyo Medical and Dental University Dental School Assistant, 歯学部, 助手 (00179665)
WATANABE Hisashi Tokyo Medical and Dental University Dental School Lecturer, 歯学部, 講師 (40143606)
HAGIWARA Satsuki Tokyo Medical and Dental University Dental School Lecturer, 歯学部, 講師 (70134715)
URAGUCHI Ryoji Tokyo Medical and Dental University Dental School Lecturer, 歯学部, 講師 (90111608)
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| Project Period (FY) |
1988 – 1989
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| Keywords | ELISA method / Serum IgG antibody / Leukocytes function / Platelet-activating-factor (PAF) / 15-HETE / Immunoblotting / Gene cloning / DNA Probe |
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| Research Abstract |
Our analysis concerning the microbiological and immunological mechanisms of periodontal diseases yielded many fruitful results. These results are as follows.
1) Serum IgG antibodies of severe periodontitis patients were measured to seven periodontopathic bacteria with enzyme-linked immunosorbent assay(ELISA).
The findings demonstrated the pathological role of B. gingivalis and A. actino mycetemcomitans played in periodontal disease.
2) The degree of definitive neutrophil chemotaxis in Down' s syndrome(DS) and its relationship to periodontal disease progression was confirmed by our studies.
3) Platelet-activating factor(PAF), a biological active lipid was detected in inflamed human gingival tissue directly associated with the occurrence and persistence of periodontal inflammation.
4) Synthesis competence of 15-hydroxy-eicosatetraenoic acid(15-HETE) by polymorphonuclear leukocytes(PMLs) of severe periodontitis patients was measured to observe 15-lipoxygenase activity. Juvenile and rapidly pro
… More
gressive periodontitis groups reported lower 15-HETE synthesis than the age-matched controls. The results are relevant to those communications concerning juvenile and rapidly progressive periodontitis with abnormal PMNL function.
5) Sonicated whole cell extracts and outer membrane proteins from B.gingivalis W50, Veionella purvula ATCC 17745 and A. actinomycetemcomitans ATCC 9710, and sonicated whole cell extract from B. gingivalis 381 were analyzed by the immunoblot technique using sera of patients with diverse periodontal diseases and control subjects.
Several major bands to which the specific antigens reacted more frequently with sera from periodontitis patients were recorded. Monitoring of antibody response to specific antigens may be significant to assess human periodontal disease progress.
6) Clones of genes with antigen reactive from sera of advanced destructive periodontitis(ADP) patients to construct a phage library of A.actinomycetemcomitans Y4 DNA were screened by the immunochemical analysis. This investigation indicated a distinctive DNA sequence encoding an A.a. Y4 antigen strongly reactive to the sera of the ADP patients. Hence, serve as a DNA probe in diagnosis of periodontitis. Less
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