• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1989 Fiscal Year Final Research Report Summary

Preliminary study for developing a new approach to treating malignant salivary gland tumors

Research Project

Project/Area Number 63480443
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 外科・放射線系歯学
Research InstitutionOsaka University

Principal Investigator

SHIRASUNA Kanemitsu  Osaka University School of Dentistry, The First Department of Oral and Maxillofacial Surgery, Associate Professor, 歯学部, 助教授 (30093420)

Project Period (FY) 1988 – 1989
KeywordsSalivary gland / Salivary neoplasms / Cytodifferentiation / Cell growth / Growth factors / Induction of differentiation / Cancer therapy
Research Abstract

Since malignant salivary gland tumors are resistance to radiotherapy and/or chemotherapy, the development of new effective treatment is required. HSG cell line of human salivary gland origin may serve as useful tool for studying the biology of the salivary neoplasms and developing a new therapy. The HSG cells have a fine structure similar to intercalated duct cells and the inoculation of the cells into athymic mice results in production of adenocarcinoma. Cells with various phenotypes including myoepithelial-like cells could be isolated from HSG by repeated single cell cloning, changes of cultured conditions or treatment with certain differentiation inducers, such as dibutyryl cyclic AMP and glucocorticoid, suggesting that HSG is pluripotential in differentiation. Induction of more differentiated stages in HSG resulted in slowing of the growth. Inhibition of tumor growth associated with cytodifferentiation is also found in vivo.
Investigation of HSG in serum free medium suggested that this cell line produce EGF and beta-TGF which are probably involved in growth mechanism as regulator. Moreover, it was found that normal human fibroblasts exert a profound influence on the growth and differentiation of HSG, by an indirect effect involving diffusible factor(s). Partial purification and characterization of the factor(s) produced by the fibroblasts suggests that it may be a novel protein carrying both tumor inhibiting and differentiation inducing activities. These results indicate that induction of cytodifferentiation in HSG is possible, suggesting a therapeutic implication for salivary gland tumors. In order to establish cancer therapy, however, further investigation of molecular mechanism on growth and differentiation regulation of HSG as well as other cell lines of salivary gland tumors is required. Such studies are presently in progress using HSG and new cell lines recently established from adenoid cystic carcinoma.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Kanemitsu SHIRASUNA et al.: "Isolation and characterization of different clones including myoepi-thelial-like variants from a clonal neopastic epithelial duct cell lines of human salivary gland origin" Cancer Research. 46. 1418-1426 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Setsuko HATAKEYAMA et al.: "Glucocorticoid-induced growth inhibition of human neoplastic salivary gland duct cell line" Acta Pathol.Jpn. 37. 587-595 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kanemitsu SHIRASUNA et al.: "Growth inhibition and differentiation of human salivary adenocarcionoma cells by medium conditioned with normal human fibroblasts" Cancer Reseach. 48. 2819-2824 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Setsuko HATAKEYAMA et al.: "Glucocorticoid-induced growth inhibition with enhanced expression of ductal epithelium of human salivary gland adenocarcinoma cells transplanted into athymic nude mice" Cancer. 62. 716-722 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kanemitsu SHIRASUNA et al.: "Different contents of glycosaminoglycans in a human neoplastic salivary duct cell line and its subclone with myoepithelial phenotype" Virchows Archiv B Cell Pathology. 57. 175-180 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kanemitsu SHIRASUNA et al.: "Bilolgical characterization of pseudocyst forming cell lines from adenoid cystic carcinoma of minor salivary gland origin" Cancer Research(in press). (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shirasuna K., Watatani K., Sugiyama M., Morioka, S., and Miyazaki, T.: "Isolation and characterization of different clones including myoepithelial-like variants from a clonal neoplastic epithelial duct cell line of human salivary gland origin." Cancer Res., 46: 1418-1426, 1986.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shirasuna K., Morioka S., Watatani K., Sugiyama M.: "Different expression of alkaline phosphatase in subclones of human neoplastic salivary duct cell line." Biochem. Biophys. Res. Comm., 138: 625-630, 1986.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hatakeyama S., Kurokawa R., Satoh M., Suzuki, A., Ota, M. and Shirasuna K.: "Glucocorticoid-induced growth inhibition of human neoplastic salivary gland duct cell line (HSG)." Acta Pathol. Jpn., 37: 587-595, 1987.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shirasuna K.: "Experimental approach to therapy of salivary gland neoplasms" J. Jpn Stomatol. Soc. 36: 982-1015, 1987.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shirasuna K., Morioka S., Watatani K., Hayashido Y., Furusawa H., Sugiyama M., Okura M., and Matsuya T.: "Growth inhibition and differentiation of human salivary adenocarcinoma cells by conditioned medium with normal human fibroblasts." Cancer Res., 48: 2819-2824, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hatakeyama S., Sashima M., Shirasuna K., Satoh, M., and Suzuki A.: "Glucocorticoid-induced growth inhibition with the enhanced expression of ductal epithelium of human salivary gland adenocarcinoma cells transplanted into athymic nude mice." Cancer (Phila.), 62: 716-722, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shirasuna K., Furusawa H., Morioka S., Watatani K., and Matsuya T.: "Different contents of glycosaminoglycans in a human neoplastic salivary duct cell line and its subclone with a myoepithelial phenotype." Virchows Arch. Cell Pathol., 57: 175-180, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shirasuna K., Watatani K., Furusawa H., Saka M., Morioka S., Yoshioka H. and Matsuya T.: "Biological characterization of pseudocyst-forming cell lines from adenoid cystic carcinomas of minor salivary gland origin." Cancer Res., 1990.

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1993-03-26  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi