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1989 Fiscal Year Final Research Report Summary

Integration of the biowarning information through the hypothalamic neuronal network.

Research Project

Project/Area Number 63480470
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 医学一般
Research InstitutionKyushu University

Principal Investigator

SHIMIZU Nobuaki  Kyushu Univ., Fac. Med., Assist. Prof., 医学部, 講師 (50019634)

Co-Investigator(Kenkyū-buntansha) OOMURA Yutaka  Kyushu Univ., Emeritus Prof., 名誉教授 (30019517)
KATAFUCHI Toshihiko  Kyushu Univ., Fac. Med., Assist. Prof., 医学部, 助手 (80177401)
HORI Tetsuro  Kyushu Univ., Fac. Med., Prof., 医学部, 教授 (00022814)
Project Period (FY) 1988 – 1989
Keywordsstress / hypothalamus / feeding behavior / serotonin / immobilization / electrochemistry / microdialysis / rat
Research Abstract

The effects of stress on food intake and on neural activity in the lateral hypothalamic area (LHA) were investigated. Significant reduction of daily food intake was observed after 2 hr immobilization. Injection of methysergide (5 mg/kg, i.p.) antagonized the immobilization-induced anorexia for 3 hr, while injection of naloxone had no effect. To study LHA neuronal responses to immobilization stress, single neuron activity was recorded by chronically implanted wire electrodes. Activity of 16 of 20 LHA neurons were significantly decreased by immobilization stress. The mean firing rate (discharges/sec) decreased from a baseline of 27.1 <plus-minus> 2.4 to 8.1 <plus-minus> 1.0 during immobilization, and the mean latency to the steady .state response was 20.1 <plus-minus> 5.2 min. No neurons increased their firing rate following immobilization in this study. Injection of methysergide (5 mg/kg, i.p.), 60 min before immobilization, completely blocked the suppressive effect of immobilization. I … More n 5 neurons tested for the effect of methysergide, all responses were antagonized by preinjection of methysergide (-67.8 <plus-minus> 5.4% change in neural discharge by immobilization without methysergide, arnd -19.6 <plus-minus> 6.2% after injection of methysergide). Further more differential pulse voltammetry with a carbon fiber electrode or brain microdialysis with high-performance liquid chromatography and electrochemical detection were used to measure 5-hydroxyindoleacetic acid (5-HIAA), the metabolic product of the serotonin. A significant increase in 5-HIAA concentration in the LHA was detected after immobilization stress. Immobilization of rats resulted in rapid increase of 5-HIAA concentration in the LHA. The increased levels reached a maximum of 46.3 <plus-minus> 8.3% 45 min after starting the immobilization. The electrochemical signals gradually decayed immediately after cessation of immobilization and returned to the preimmobilization level within 180 min. These results suggest that immobilization-induced anorexia is mediated at least in partly through serotoninergic mechanisms in the h Less

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Shimizu,N.: "Stress-induced anorexia in rats mediated by serotonergic mechanisms in the hypothalamus." Physiol.Behav.46. 835-841 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimizu,N.: "Electrochemical analysis of hypothalamic serotonin metabolism accompanied by immobilization stress in rats." Physiol.Behav.46. 829-834 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Minami,T.: "Hypothalamic neuronal activity changes to 3-hydroxybutyric acid,an endogenous organic acid." Brain Res.509. 351-354 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ono,K.: "Fetal hypothalamic brain grafts to the ventromedial hypothalamic obese rats:an immnohistochemical,electrophysiological and behavioral study." Brain Res.Bull.24. 89-96 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimizu,N.: "Hypothalamic microdialysis of mazindol causes anorexia with increased in synaptic serotonin in rats." Submitted.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimizu,N.: "Glutamete modulates dopamine release in the striatum as measured by brain microdialysis." Submitted.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimizu, N. et al.: "Stress-induced anorexia in rats mediated by serotonergic mechanisms in the hypothalamus." Physiol. Behav., 46, 835-841, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimizu, N. et al.: "Electrochemical analysis of hypothalamic serotonin metabolism accompanied by immobilization stress in rats." Physiol. Behav., 46, 829-834, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ono, K. et al.: "Fetal hypothalamic brain grafts to the ventromedial hypothalamic obese rats: an immunohistochemical, electrophysiological and behavioral study." Brain Res. Bull., 24 89-96, 1990.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minami et al.: "Hypothalamic neuronal activity changes to 3-hydroxybutyric acid, an endogenous organic acid." Brain Res., 509, 351-354, 1990.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimizu, N. et al.: "Hypothalamic microdialysis of mazindol causes anorexia with increase in synaptic serotonin in rats."

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimizu, N. et al.: "Gulutamate modulates dopamine release in the striatum as measured by brain microdialysis."

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Duan, S., et al.: "Hyperpolarizing action of enkephalin on neurons in the dorsal motor nucleus of the vagus in vitro."

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Aoyagi, K. et al.: "Serotoninergic modulation of feeding behavior: analysis with in vivo voltammetry and simultaneous recording of single neuron activity in the lateral hypothalamus."

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-26  

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