Studies on production of new stock (Matthiola incana R.Br) varieties with dark yellow-colored flowers.

1989 Fiscal Year Final Research Report Summary

• Project/Area Number: 63560027
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: 園芸・造園学
• Research Institution: Kobe University
• Principal Investigator: INAGAKI Noboru Kobe Univ. Fac. of Agri. Assistant, 農学部, 助手 (30151575)
• Project Period (FY): 1988 – 1989
• Keywords: Matthiola incana R.Br / Cheiranthus cheiri L. / yellow flower / protoplast / cell fusion / micropropagation / morphogenesis / in vitro fertilization

Research Abstract

The following experiments were carried out to produce new varieties of stock(Hatthiola incana R.Br) with dark yellow-colored flowers through crossing between stock and-wall flower(Cheiranthus cheiri L.), fertilized ovule cultuie, in vitro fertilization, and protoplast fusion.
The micropropagation was also applied to obtain a number of the stock plants which were useful for commercial markets.
1.Crossing between stock and wall flower: The characters and flowering earliness of most of the plants, which were obtained from crossing between the above two plants, were similar to those of the female parents in crossing. The flower color(white) of the plants, obtained through the cross M.ineana cv.'Shiho'x C.cheiri cv.'Aliony orange' was different from that (violet) of the female parent, as well as that (pale yellow) of M.incana cv.'Sosetsu' C.cheiri cy.'all double' was different from that(white) of the female parent.
2.Protoplast isolation and culture from stock callus: The yield of protoplast f rom stock callus under the light condition was higher than that under the dark condition. However, prtoplasts from stock callus under dark condition continued to divide and grew into colonies, and calluses on the MS modified medium with 0.3 mg/l BA after 3 months of incubation.
3.Electrofusion of Protoplasts: The optimal conditions for the formation of pearl chains of protoplasts and the fusin of protoplasts were as follows respectively : a protoplast density of 105 /ml and a high frequency electric field of 150 v/cm under a alternative frequency of 1 MHz for 20 sec., and a pulse field strength of 1.5 Kv/ cm and a pulse width of 70 mu s.
4.Micropropagation of stock with double flowers through tissue culture :Leaf and hypocotyl sections and hypocotyl-derived calluses could be applied as prominent explants for in vitro shoot formation when cultured on 1/2MS modified medium supplemented with BA or zeatin (i mg/1), and L-glutamate (30 mm) in compensation for NH_4NO_3 and kNO_3 as a nitrogen source.