1989 Fiscal Year Final Research Report Summary
Studies on Novel Syntheses of Useful Carbohydrates by Enzyme Hybrid
| Project/Area Number |
63560124
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
製造化学・食品
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| Research Institution | Shizuoka University |
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Principal Investigator |
USUI Taichi Shizuoka University, Faculty of Agriculture; Professor, 農学部, 教授 (50111802)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIDO Yoshiharu Tokyo Pharmaceutical University; Professor, 教授 (60016037)
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| Project Period (FY) |
1988 – 1989
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| Keywords | Lysozyme / Chitinase / Enzyme Hybrid / Transglycosylation / Synthesis Of Useful Oligosaccharide |
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| Research Abstract |
This project is concerned with the enzymatic syntheses of useful chitinoligo- saccharides by chitinolytic enzymes p-Nitrophenyl penta-N-acetyl-beta-chitopentaoside, which is available as a substrate for a lysozyme assay, was efficiently synthesized by transfer of the tetra-N-acetyl-chitotetraosyl residue to the 4-position of p- nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside using lysozyme in an aqueous solution containing DMSO in a high concentration. Also, a chitinase from Trichoderma reesei catalyzed a transglycosylation reaction from tetra-N-acetyl-chitotetraose as a substrate to hexa-N-acetyl-chitohexaose. The transglycosylation by lysozyme furthermore afforded a more convenient way of preparing hexa-N-acetyl-chitohexaose and hepta-N-acetyl-chitoheptaose from di-N-acetyl-chitobiose than the chitinase reaction.
Enzyme hybrid was prepared by shiff-amino reduction of hen egg-white lysozyme with lactose. The modified lysozyme gained more temperature stability and resistance to organic solvent than the native one.
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