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1990 Fiscal Year Final Research Report Summary

Immunological and Biochemical Studies on a Hemolysin Produced by Aeromonas Salmonicida

Research Project

Project/Area Number 63560196
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General fisheries
Research InstitutionKitasato University

Principal Investigator

NOMURA Setsuzo  Kitasato University, Department of Fisheries Sciences, Professor, 水産学部 (30050404)

Co-Investigator(Kenkyū-buntansha) KAWAHARA Eijiro  Kitasato University, Department of Fisheries Sciences, Research Associate, 水産学部, 助手 (80186121)
Project Period (FY) 1988 – 1990
KeywordsFurunculosis / Aeromonas Salmonicida / Hemolysin / Toxoid / Vaccine / Enzyme Linked Immuno-Sorbent Assay / Anti-toxin / Asialoglycophorin
Research Abstract

The 50 % lethal doses (LD_<50>) of purified salmolysin for white-spotted char on intramuscular and intraperitoneal injection were 196 and 556 mu/kg. The LD_<50> for rainbow trout on intramuscular injection was 58 mu/kg. Salmolysin was detoxificated by heat-treatment at 60 ^0C for 30 min, or by formalin-treatment for 1 week. Salmolysin toxoid was injected to white-spotted char intramuscularly. As the result, agglutinating antibody titer was reached to maximum after 3 weeks. White-spotted char were immunized with salmolysin toxoid by intramuscular injection or immersion method, after, that, the fish were cahllenged with Aeromonas salmonicida. AS the result, survival ratio of immunized fish was higher than that of non-immunized fish. These results indicate that salmolysin toxoid is an effective immunogen against furunculosis. Salmolysin antitoxin was purified from the serum of white-spotted char by ammonium sulfate precipitation, anion-exchange chromatography on DEAE-cellulose, and gel filtration on Sephatose 6B. Furthermore, salmolysin produced in the culture supernatant of A. salmonicida and in the focus tissue of infected fish was detected by enzyme linked immuno-sorbent assay. On the other hand, the red blood cells of salmonids were peculiarly lysed by salmolysin, however, the red blood cells of fowls and mammalia were not lysed by the toxin. Since the sensitivity of salmonid red blood cells to the toxin was different, it was suggested that the surface structure of salmonid erythrocytes varies with the genus level. Salmolysin did not show phospholipase C activity and was not reactivated by thiolcompound. The hemolytic catibity of salmoysin was not affected by phospholipids, however, the activity was stimulated by asialoglycophorin.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Eijiro Kawahara and Setsuzo Nomura: "Lethal toxicity and immurogenicity of salmolysin,Aeromonas salmonicida hemolysin to whiteーspotted char,Salvelinus leucomaenis." Fish Pathology. 24. 149-154 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Eijiro Kawahara and Setsuzo Nomura: "Lethality and immurogenicity of Aeromonas salmonicida extracellular products to salmonids." Proceedings of The Second Asian Fisheries Forum. 671-674 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Eijiro Kawahara, Shinーichiro Oshma and Setsuzo Nomura: "Separation of lymphocytes from peripheral blood of coho salmon Oncorhynchus kisutch." Nippon Suisan Gakkaishi. 56. 363 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Eijiro Kawahara,Shunーichiro Oshima and Setsuzo Nomura: "Toxicity and immunogenicity of Aeromonas salmonicida extraソellular products to salmonids." Journal of Fish Diseases. 13. 495-503 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Eijiro Kawahara,tomohiro Ueda and Setsuzo Nomura: "Injection vaccination of whiteーspotted char,Salvelinus leucomaenis,against furnnculosis with Aeromonas salmonicida salmolysin." Fish Pathology. 26. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Eijiro Kawahara,Teruaki Inarimori,Setsuzo Nomura and Yukinori Takahashi: "Humoral immure response of whiteーspotted char,Salvelinus leucomaenis,to Aeromonas salmonicida extrecellnlar products." Nippon Suisan Gakkaishi.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawahara, E. and S. Nomura: "Lethal toxicity and immunogenicity of salmolysin, Aeromonas salmonicida hemolysin to white-spotted char, Salvelinus leucomaenis." Fish Pathol. 24(3). 149-154 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawahara, E. and S. Nomura: "Lethality and immunogenicity of Aeromonas salmonicida extracellular products to salmonids." "Proceedings of The Second Asian Fisheries Forum.671-674 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawahara, E., S. Oshima, and S. Nomura: "Separation of lymphocytes from the peripheral blood of coho salmon Onchorhynchus kisutch." Nippon Suisan Gakkaishi. 56(2). 363 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawahara, E., S. Oshima, and S. Nomura: "Toxicity and immunogenicity of Aeromonas salmonicida extracellular products to salmonids." J. Fish Dis.13(6). 495-503 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawahara, E., T. Ueda, and S. Nomura: "Infection vaccination of white-spotted char, Salvelinus leucomaenis, against furunculosis with Aeromonas salmonicida salmolysis." Fish Pathol. 26 (1). (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawahara, E., T. Inarimori, S. Nomura, and Y. Takahashi: "Humoral immune response of white-spotted char, Salvelinus leucomaenis, to Aeromonas salmonicida extracellular products." Nippon Suisan Gakkaishi.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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