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1989 Fiscal Year Final Research Report Summary

Studies on Fish Sarcoplasmic Reticulum

Research Project

Project/Area Number 63560198
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Fisheries chemistry
Research InstitutionThe University of Tokyo

Principal Investigator

WATABE Shugo  Instructor, Fac. Agric., Univ. Tokyo, 農学部, 助手 (40111489)

Project Period (FY) 1988 – 1989
KeywordsSarcoplasmic reticulum / Plaice / Carp / Ca^<2+> uptake / Rigor mortis / Temperature acclimation / Storage experiment / Ca^<2+> pump
Research Abstract

Sarcoplasmic reticulum (SR) is an organ which controls muscle intracellular Ca^<2+> concentration and found in fish muscle. This study deals with characterization of SR from a few fishes and its possible role in rigor mortis progress.
1. Spiked plaice was stored at 0 or 10゚C and examined for changes in Ca^<2+> concentration in the ordinary muscle SR by electron microscopic observation. Ca^<2+> in SR disappeared faster at 0゚C than at 10゚C with a faster development in rigor tension at 0゚C. In addition, fragmented SR prepared from plaice showed the lowest Ca^<2+> uptake when measured at 0゚C.
2. Plaice myofibrillar Mg^<2+>-ATPase activity was lower at a reaction temperature of 0゚C than at 10゚C in the absence of Ca^<2+>, the condition corresponding to a relaxed state of muscle. Since the previous results suggest that intracellular Ca^<2+> concentration increases quickly when plaice is stored at 0゚C, myofibrillar Mg^<2+>-ATPase activity was measured at this temperature in the presence of Ca^<2+>. The activity was higher than that at 10゚C in the absence of Ca^<2+>.
3. Fragmented SR from carp ordinary muscle was further purified into heavy and light SR fractions. HSR fraction contained many round vesicles, some of them with feet structures on the surface. SDS-gel electrophoresis revealed that carp SR was composed mainly of Ca^<2+> pump protein with a subunit molecular weight of 95,000 (95kDa) and of 70kDa, 35kDa and 10kDa Ca^<2+>-binding proteins.
4. Carp acclimated to 30゚C was faster in rigor mortis progress than that acclimated to 10゚C when stored at 0゚C after spiking. Correspondingly, Ca^<2+> uptake by SR from warm-acclimated carp was lower at a reaction temperature of 0゚C than that from cold-acclimated carp.
5. The above results suggest that fish SR plays an important role in rigor mortis in terms of affecting intracellular Ca^<2+> concentration after death.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Watabe,Shugo: "Temperature-dependency of rigor-mortis of fish muscle:Myofibrillar Mg^<2+>-ATPase activity and Ca^<2+> uptake by sarcoplasmic reticulum" Journal of Food Science. 54. 1107-1110 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ushio,Hideki: "Purfication and characterization of carp sarcoplasmic reticulum" Nipppon Suisan Gakkaishi. 55. 1641-1648 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 渡辺終五: "最近の魚貝類の鮮度保持研究-死後硬直を中心にして" 魚肉ソ-セ-ジ. No.221. 15-29 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Watabe,Shugo: "Changes in rigor-mortis progress of carp induced by temperature acclimation" Agricultural and Biological Chemistry. 54. 219-221 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Watabe,Shugo: "Biochemical responses to thermal acclimation in muscle proteins of carp" Proceedings of First International Marine Biotechnology Conference.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Watabe, Shugo: "Temperature-dependency of rigor-mortis of fish muscle: Myofibrillar Mg^<2+> -ATPase activity and Ca^<2+> uptake by sarcoplasmic reticulum." J. Food Sci., 54, 1107-1110(1989).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ushio, Hideki: "Purification and characterization of carp sarcoplasmic reticulum." Nippon Suisan Gakkaishi, 55, 1641-1648(1989).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watabe, Shugo: "Recent advances on the preservation of fish and shellfish with special reference to rigor mortis." Fish Sausage, No.221, 15-29(1989).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watabe, Shugo: "Changes in rigor-mortis progress of carp induced by temperature acclimation." Agric. Biol. Chem., 54, 219-221(1990).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watabe, Shugo: "Biochemical responses to thermal acclimation in muscle proteins of carp." Proc. 1st Internat. Mar. Biotechnol. Conf.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-26  

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