1989 Fiscal Year Final Research Report Summary
Experimental studies on the mechanism of lymph follicle formation
| Project/Area Number |
63570019
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Nihon University |
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Principal Investigator |
HOSHI Hajime Nihon Univ., Sch. Med., Dep. Anat., Professor, 医学部, 教授 (30059290)
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| Co-Investigator(Kenkyū-buntansha) |
HORIE Kaeko Nihon Univ., Sch. Med., Dep. Anat., Assistant Lecturer, 医学部, 助手
NAGATA Hidetsugu Nihon Univ., Sch. Med., Dep. Anat., Assistant Lecturer, 医学部, 助手 (00102525)
TAKEMOTO Ritsuko Nihon Univ., Sch. Med., Dep. Anat., Lecturer, 医学部, 講師 (60059251)
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| Project Period (FY) |
1990 – 1991
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| Keywords | Lymph follicle formation / Antigen stimulation / X-irradiation / Athymic nude mice / Follicle formation in developing lymph nodes / Primary and secondary antigenic challenge / Sites of lymph follicle formation |
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| Research Abstract |
The present project was designed to test our hypothesis that lymph follicle formation is triggered by substances which stimulate either T or B lymphocytes and concomitantly activate macrophages, and to gain some insight into the mechanism of lymph follicle formation in the lymph node.
1. Lymph follicle formation was effectively induced in mouse draining lymph nodes following local injection of certain types of antigen. but this was markedly inhibited when whole-body X-irradiation (500 rads) was given before or after the antigen stimulation, whereas it was not in the case local X-irradiation was given in stead of whole-body X-irradiation. The results indicated that the lymphocyte is required for lymph follicle formation.
2. When locally injected into athymic nude mice, precipitated or high-molecular, thymus-independent antigens were effective in inducing follicle formation in draining lymph nodes, whereas soluble thymus-independent antigens and precipitated or high-molecular-weight, thymus-dependent antigens were ineffective. The results support the hypothesis that lymph follicle formation is triggered by substances which stimulate B or T lymphocytes and concomitantly activate macrophages.
3. Mice were locally injected with effective antigens at various postnatal ages and the formation of lymph follicles in draining lymph nodes were studied. The results showed that the mouse popliteal lymph node began to respond to thymus-dependent antigens at around 3 days after birth, whereas they began to respond to thymus-independent antigens at around 5 days of age.
4. Induction of lymph follicle formation in draining nodes following the primary and secondary local challenge with antigens was studied. It was found that antigens which triggered the formation of lymph follicles in the primary challenge could evoke lymph follicle formation more efficiently in the secondary challenge.
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