1989 Fiscal Year Final Research Report Summary
ELECTROPHYSIOLOGICAL PROPERTIES AND OSMOSENSITIVITIES OF ISOLATED HYPOTHALAMIC NEURONS.
| Project/Area Number |
63570072
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | UNIVERSITY OF OCCUPATIONAL AND ENVIRONMENTAL HEALTH, SCHOOL OF MEDICINE |
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Principal Investigator |
INENAGA Kiyotoshi UNIVERSITY OF OCCUPATIONAL AND ENVIRONMENTAL HEALTH, SCHOOL OF MEDICINE, ASSISTANT PROFESSOR, 医学部, 助手 (90131903)
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| Project Period (FY) |
1988 – 1989
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| Keywords | HYPOTHALAMUS / PATCHCLAMP METHOD / OSMOSENSITIVITY / SLICE / OPIOID |
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| Research Abstract |
1. To test osmosensitivity of hypothalamic neurons, the patch clamp method and the concentration clamp were used in the present study. Whole cell recordings were obtained from neurons in the regions of the anteroventral third ventricle (AV3V) which were isolated enzymatically and mechanically from 7 to 15-day-old rats. We analyzed chance of voltage-dependent sodium current with osmotic challenge. The results demonstrated the view that osmotic stimulation acts on neurons located in the AV3V region by modifying their membrane permeability for sodium ions with a slow kinetics.
2. To clarify opioid-response of supraoptic neurons in the hypothalamus, slice preparation and extracellular recordings were used. The results suggest that opioids acting preferentially at kappa-receptors inhibit both vasopressin and oxytocin neurons while delta- and mu-receptor agonists inhibit primarily oxytocin neurons.
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