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1989 Fiscal Year Final Research Report Summary

Mechanism of the production of two isozymes of fumarase encoded from a single gene

Research Project

Project/Area Number 63570107
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionYamagata University

Principal Investigator

YOSHIDA Tadashi  School of Med. Dept. of Molecular and Pathological Biochemistry, Professor, 医学部, 教授 (10004673)

Co-Investigator(Kenkyū-buntansha) SATO Michihiko  School of Med. Dept. of Molecular and Pathological Biochemistry, Associate Profe, 医学部, 助教授 (00135344)
Project Period (FY) 1988 – 1989
KeywordsFumarase / Isozyme / cDNA for fumarase / Production of fumarase
Research Abstract

Rat liver contained two isozymes of fumarase which are localized in different intracellular compartments, the mitochondria and cytosol. These isozymes are almost equally distributed between these two subcellular compartments, and cannot be differentiated from each other except that the NH_2-terminal amino acid of the cytosolic isozyme was acetylated. In the rat liver it is suggested that the cytosolic and mitochondrial fumarases are products of the same gene. Therefore, in this study we intended to examine the mechanism how the two isozymes of fumarase are produced from the same gene and found the following observations.
cDNA clone for fumarase with an insert of about 1.7Kb was isolated from a rat liver cDNA library. Nucleotide sequence analysis of the insert revealed that the cDNA contained a 5'-noncoding region of 25 nucleotides, the coding region of 1521 nucleotides, and a 3'-nontranslated region of 43 nucleotides fallowed by a poly(A)^+ tail. The open reading frame encoded a polypeptide of 507-amino acid residues, which contained an additional sequence (so-called presequence) of 41-amino acid residues. Interestingly, there was another initiation codon AUG just upstream of GCA, which is the codon of the NH_2-terminal Ala of both mature enzymes. Therefore, if translation is initiated from this second initiation codon, the precursor of the cytosolic fumarase will be synthesized by the same mRNA as mitochondrial enzyme. Another experimental findings obtained by hybrid- selected translation, Northern and Southern blot, primer-extension analysis, and S-1 nuclease mapping analysis also supported the above possibility.
Recently we isolated a genomic clone for rat fumarase. Now we are trying to analyze the structure of the genomic DNA to obtain information on the mechanism of synthesis of two kinds of precursor polypeptides.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Tamio Suzuki: "Rat liver mitochondrial and cytosolic fumarases with identical amino acid sequences are encoded from a single gene" J.Biol.Chem.264. 2581-2586 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Syozo Tuboi: "Rat liver mitochondrial and cytosolic fumarases with identical amino acid sequences are encoded from a single mRNA with two alternative in-phase AUG initiation site" Advan.Enzyme Regul.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tamio Suzuki, Michihiko Sato, Tadashi Yoshida, Syozo Tuboi: "Rat liver mitochondrial and cytosolic fumarases with identical amino acid sequences are encoded from a single gene" J. Biol. Chem. 264 (5), 2581-2586, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Syozo Tuboi, Tamio Suzuki, Michihiko Sato, Tadashi Yoshida: "Rat liver mitochondrial and cytosolic fumarases with identical amino acid sequences are encoded from a single mRNA with two alternative in-phase AUG initiation site" Advan. Enzyme Regul.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-26  

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