1989 Fiscal Year Final Research Report Summary
Immunohistochemical localization of catecolamine synthesizing enzymes in human pheochromocytomas
| Project/Area Number |
63570149
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Human pathology
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| Research Institution | Tokai University School of Medicine |
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Principal Investigator |
OSAMURA Yoshiyuki Tokai University Department of Pathology Professor, 医学部, 教授 (10100992)
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| Co-Investigator(Kenkyū-buntansha) |
TSUTUMI Yutaka Tokai University Department of Pathology, 医学部, 講師 (80138643)
SATO Shinkichi Tokai University Department of Pathology, 医学部, 講師 (80119677)
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| Project Period (FY) |
1988 – 1989
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| Keywords | Pheochromocytoma / Adrenalin / Noradrenaline / Phenylethanolamine beta-methyltransferase / Dopamine beta-hydroxylase / Immunohistochemistry / Tyrosine beta hydroxylase |
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| Research Abstract |
Abstract Total 17 (10 adrenal and 7 extra-adrenal) pheochromocytomas were examined immunohistochemically for the localization of catecholamine synthesizing enzymes, throsine hydroxylase (TH), dopamine 3-hydroxylase (DBH) and phenylethanolamine beta-methyltransferase (PNMT) as well as the marker peptides for pheochromocytomas, i.e. met-enkephalin-arg-gly-leu (MEAGL). The normal adult, fetal, newborn human adrenal medullae were also examined.
6 adrenal pheochromocytomas showed immunohistochemical positive staining for PNMT, 5 of these cases demonstrated elevated serum adrenalin levels. This indicated morpho-functional correlation. PNMT positive cells were mostly positive for TH, but did not show colocalization of MEAGL except for one case. Absence of colocalization was considered to reflect physiological condition from the similar staining in the normal adult adrenal medulla. Primary culture and immunoelectron microscopy suggested the processing and synthesis of adrenalin and MEAGL in the secretory granules.
7 extra-adrenal pheochromocytomas were negative for PNMT and suggested the lack of adrenalin synthesis.
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[Publications] Osamura,Yoshiyuki.,Kawai,K.,Hori,S.,Suemizu,H.,Onoda,N.,Tong H.Joh: "Immunohistochemical Localization of Catecholamine synthesizing enzymes in human pheochromocytomas" Endocrine Pathology.
Description
「研究成果報告書概要(和文)」より
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[Publications] Osamura, Y., Kawai, K., Hori, S., Suemizu, Y., Onoda, N. and Joh, T.H.: "Immunohistochemical localization of catecholamine synthesizing enzymes in human pheochromocytomas." Endocrine Pathology 1990.
Description
「研究成果報告書概要(欧文)」より
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[Publications] Osamura, Y., Suemizu, Y., Yasuda, N., Hiro, S., Onoda, N. and Joh, T.H.: "Immunohistochemical studies of catecholamine synthesis in human pheochromocytomas." Folia Endocrinol Japon 65:205 (Abstr), 1989.
Description
「研究成果報告書概要(欧文)」より
