1989 Fiscal Year Annual Research Report

人及びラットの肝臓細胞癌、肝内胆管癌の前駆病変細胞核DNAploidyに関する研究

Research Project

Project/Area Number	63570158
Research Institution	Gifu University
Principal Investigator	森秀樹岐阜大学, 医学部, 教授 (70021433)
Co-Investigator(Kenkyū-buntansha)	加藤一夫岐阜大学, 医学部, 助教授 (70115400) 田中卓二岐阜大学, 医学部, 助手 (40126743)
Keywords	人肝癌 / 細胞核DNAploidy / 腺腫様過形成 / 変異増殖巣 / 肝発癌物質 / ラット
Research Abstract	人肝癌の前駆病変の1つとして最近腺腫様過形成(adenomatoid hype-rplasia(肝硬変を背景に出現する)の存在が注目されている。この様な病変の細胞核DNAploidyパタ-ンの解析を組織切片にてFeilgen反応標本によるMMSP(オリンパス)の計測にて解析した。これらの症例肝細胞核DNAploidyパタ-ンは2.5〜5cをモ-ドし1.5〜9.5cの拡がりを有するのであった。非病変細胞核ploidyパタ-ンはこれに対し、2c〜3cにモ-ドを有し、1c〜6cの拡がり有するものであった。この成績は相対的に腺腫様過形成細胞は非病変細胞核に比し、相対的に高いDNAcontectを有することを示すものと考えられた。先に我々はN-2-flciorevorylacetamide(FAA)の投与により誘発されるラット肝細胞性特異増殖巣細胞核DNAploidyパタ-ンが非病変細胞核ploidyパタ-ンに比し拡がりを有し、DNA量の相対的増加を示すことを報告したが、その後の幾つかの報告は逆に変異増殖巣肝細胞はploidy reductionを有し、diploidの細胞が多いことを指摘している。最近我々はFAA、methylazoxymethanol acetate、N-nitrosopyrrolizicline、aflatoxinB1、5、6-dimethoxysterigmatocystin、diethylnitros-amine(DEN)、dimethoxylaminoagobenzeneなどの肝発癌物質によるラットの異なったモデルに誘発された種々の変異増殖巣細胞核DNAploidyパタ-ンを比較解析した。その結果、DENなどにより短期にin-itiateしたモデルに出現する変異増殖巣はdiploidパタ-ンに近いDNAモ-ドを示すことが多く、FAAなどの飼料投与により、比較的長いinitiarionにより造られる変異増殖巣ではploidyパタ-ンの拡がるtypeが増加することが分かった。この様にモデルの種類により異なったDNAploidyの変異増殖巣が出現するなど変異増殖巣の多様性が明らかとなり、安易に比較を論じられないことが分かった。

Research Products
(8 results)

All Other

All Publications (8 results)

[Publications] Mori,H.,Tanaka,T.,N.,iwa,K.,Yoshimi,NIwata,H.,Hara,A.: "Induction of altered hepatocellular foci of hamster for a possible short-term assay for carcinogens" Research Communications in Chemical Pathology and Pharmacology. 63. 451-454 (1989)
[Publications] Tanaka,T.,Nishikawa,A.,Iwata,H.,Mori,Y.,Hara,A.,Mori,H.: "Enhancing effect of ethanol on aflatoxin-B1-induced hepatocarcinogenesis in male ACI/N rats" Japanese Journal Cancer Research. 80. 526-530 (1989)
[Publications] Tanaka,T.,Takeuchi,T.,Nishikawa,A.,Takami,T.,Mori,H.: "Nucleolar organizer regions in hepatocarcinogenesis induced by N-2-fluorenylacetamide in rats:Comparison with bromodeoxyuridine immunohistochemistry" Japanese Journal Cancer Research. 80. 1047-1051 (1989)
[Publications] Hara,A.,Sakai,N.,Yamada,H.,Tanaka,T.,Kato,K.,Mori,H.,Sato,K.: "Induction of glutathione S-transferase,placental type in T9 glioma cell by dibutyryladenosine 3',5'-cyclic monophosphate and modification of its expression by naturally occurring isothiocyanates" Acta Neuropathologica. 79. 144-148 (1989)
[Publications] Williams,G.M.,Mori,H.,McQueen C.A.: "Structure-activity relationship in the rathepatocyte DNA-repair test for 300 chemicals" Mutation Research. 221. 263-286 (1989)
[Publications] Mori,H.,Yoshimi,N.,Iwata,H.,Mori,Y.,Hara,A.,Tanaka、T.,: "Carcinogenicity of naturally occurring 1-hydroxyanthraquinone in rats:induction of larqe bowel,liver and stomach neoplasms" Carcinogenesis. (1990)
[Publications] Mori,H.,Ichida,H.,Tanaka,T.,Williams,G.M.: "Pathological features of preneoplastic and neopastic liver lesions in rodents and Humans.In Comparative U1trastructural Pathology of Selected Tumors in Man and Animals ed by H.M.Schuller" CRC Press Inc.,Boca Raton,Florida, 61-96 (1989)
[Publications] Mori,H.,Kawai,K.: "Genotoxicity in rodent hepatocytes and carcinogenicity of mycotoxins and related chemicals.In Mycotoxins and phycotoxins '88. ed by S.Natori,K.Hashimoto,Y.Ueno" Elsevier Science PUB1. B.V.Amsterdam, 81-90 (1989)

1989 Fiscal Year Annual Research Report

人及びラットの肝臓細胞癌、肝内胆管癌の前駆病変細胞核DNAploidyに関する研究

Principal Investigator

森 秀樹 岐阜大学, 医学部, 教授 (70021433)

Research Products

[Publications] Mori,H.,Tanaka,T.,N.,iwa,K.,Yoshimi,NIwata,H.,Hara,A.: "Induction of altered hepatocellular foci of hamster for a possible short-term assay for carcinogens" Research Communications in Chemical Pathology and Pharmacology. 63. 451-454 (1989)

[Publications] Tanaka,T.,Nishikawa,A.,Iwata,H.,Mori,Y.,Hara,A.,Mori,H.: "Enhancing effect of ethanol on aflatoxin-B1-induced hepatocarcinogenesis in male ACI/N rats" Japanese Journal Cancer Research. 80. 526-530 (1989)

[Publications] Tanaka,T.,Takeuchi,T.,Nishikawa,A.,Takami,T.,Mori,H.: "Nucleolar organizer regions in hepatocarcinogenesis induced by N-2-fluorenylacetamide in rats:Comparison with bromodeoxyuridine immunohistochemistry" Japanese Journal Cancer Research. 80. 1047-1051 (1989)

[Publications] Williams,G.M.,Mori,H.,McQueen C.A.: "Structure-activity relationship in the rathepatocyte DNA-repair test for 300 chemicals" Mutation Research. 221. 263-286 (1989)

[Publications] Mori,H.,Yoshimi,N.,Iwata,H.,Mori,Y.,Hara,A.,Tanaka、T.,: "Carcinogenicity of naturally occurring 1-hydroxyanthraquinone in rats:induction of larqe bowel,liver and stomach neoplasms" Carcinogenesis. (1990)

[Publications] Mori,H.,Ichida,H.,Tanaka,T.,Williams,G.M.: "Pathological features of preneoplastic and neopastic liver lesions in rodents and Humans.In Comparative U1trastructural Pathology of Selected Tumors in Man and Animals ed by H.M.Schuller" CRC Press Inc.,Boca Raton,Florida, 61-96 (1989)

[Publications] Mori,H.,Kawai,K.: "Genotoxicity in rodent hepatocytes and carcinogenicity of mycotoxins and related chemicals.In Mycotoxins and phycotoxins '88. ed by S.Natori,K.Hashimoto,Y.Ueno" Elsevier Science PUB1. B.V.Amsterdam, 81-90 (1989)

森秀樹岐阜大学, 医学部, 教授 (70021433)