1990 Fiscal Year Annual Research Report

人及びラットの肝細胞癌,肝肉胆管癌の前駆病変細胞核DNA ploidyに関する研究

Research Project

Project/Area Number	63570158
Research Institution	Gifu University
Principal Investigator	森秀樹岐阜大学, 医学部, 教授 (70021433)
Co-Investigator(Kenkyū-buntansha)	杉江茂幸岐阜大学, 医学部・附属病院, 助手 (60187648) 田中卓二岐阜大学, 医学部, 講師 (40126743)
Keywords	細胞核DNA ploidy / 変異増殖巣 / Microspectrophotometer / SoltーFarberモデル / Nーαーfluorenylacetamide / ラット
Research Abstract	先にNーαーfluorenylacetamideCFAA)の投与により誘発されるラット肝細胞性変異増殖巣細胞核DNA ploidyパタ-ンが非病変肝細胞核DNA ploidyパタ-ンに比し,拡がりを有し,DNA量の相対的増加を示すことを報告したが,その後の幾つかの報告は逆に変異増殖巣肝細胞がploidy reductionを有し,diploidの細胞が増加することを指摘している。我々は変異増殖単細胞核DNA量は病変の誘発方法により多様性を示すものと推論している。今回,2つの肝発癌モデルを用いて病変を誘発し,変異増殖巣細胞核DNA量の比較解析を行った。第I群では病変はSoltーFooberのモデルにより得られた(diethylnitrosamine 200mg/kg1回投与,0.02%FAA飼料投与,2/3部分肝切除を行った)。第II群では変異増殖巣は0.02%FAAの飼料投与のみになって得られた。この様なラットモデルにより誘発された肝病変を特に経時的に動物を屠殺した材料についてFeulgen染色切片標本にてmicrospectrophotomater(MMSP)を用いて比較観察した。第I群に出現した変異増殖巣はほとんどが好酸性型で出現頻度は経時的に減少し,diethylnitrosamine投与後6週から8週にかけて35.0/cm^2→15.8/cm^2の如く変化した。第II群は於いても出現増殖巣の多くは好酸性型であり、経時的な出現頻度の変化は第I群と異なり、FAA投与12週まで増加し、その後漸減するものであった。第I群に於いて,好酸性変異増殖巣は時間の経過と共に相対的DNA量の低下を示し,diploid化を惹起した。一方,第II群に誘発された好酸性変異増殖巣のDNA量の経時的変化は第I群と少々異なり,FAA投与6週後と16週では本質的な差異はみられなかった。第II群に主として観察されたtoluidine blue陽性の超塩基型変異増殖巣細胞核DNA ploidyパタ-ンはaneceploidyを示した。変異増殖巣の細胞核DNA ploidyパタ-ンは病変の種類の他,誘発モデルの相違,時間的経過等により異なりを示すことが確認された。

Research Products
(8 results)

All Other

All Publications (8 results)

[Publications] Mori,H.,Yishimi,N.,Iwata,H.,Mori,Y.,Hara,A.,Tanaka,T.et al.: "Carcinogenicity of naturally occurring 1ーhydroxyanthraquinone in rats:induction of large bowel,liver and stomach neoplasms" Carcinogenesis. 11. 799-802 (1990)
[Publications] Mori,H.,Iwata,H.,Morishita,Y.,Mori,Y.,Ohno,T.,Tanaka,T.,Sasaki,S.: "Synergistic effect of radiation on Nー2ーfluorenylacetーamideーinduced hepatocarcinogenesis in male ACI/N rats" Japanese Journal of Cancer Research. 81. 975-978 (1990)
[Publications] Hara,A.,Yamada,H.,Sakai,N.,Hirayama,H.,Tanaka,T.,Mori,H.: "Immunohistochemical demonstration of the placental form of glutathione Sーtransferase,a detoxifying enzyme in human gliomas" Cancer. 66. 2563-2568 (1990)
[Publications] Tanaka,T.,Kojima,T.,Okumura,A.,Yoshimi,N.,Mori,H.: "Alterations of the nucleolar organizer regions during 4ーnitroquinoline 1ーoxideーinduced tongue carcinogenesis in rats" Carcinogenesis. 12. 329-333 (1991)
[Publications] Mori,Y.,Yoshimi,N.,Iwata,H.,Tanaka,T.,Mori,H.: "The synergistic effect of 1ーhydroxyanthraquinone on methylazoxymethanol acetateーinduced carcinogenesis in rats" Carcinogenesis. 12. (1991)
[Publications] Mori,H.,Sugie,S.,Okumura,A.,Hara,A.,Kinouchi,T.,Kataoka,K.,Ohnishi,Y.: "Genotoxicity of pyrene oxide and 1ーnitropyrene oxides in hepatocyte primary culture/DNA repair test" Mutation Research. (1991)
[Publications] Mori,H.: "Genotoxicity of naturallyーoccurring metabolites:structural analogs of aflatoxin and related chemicals.In Handbook of Applied Mycology,Vol.5 ed by D.Bhatnagar et al." Marcel Dekker Inc.,New York, (1991)
[Publications] Kawai,K.,Hisada,K.,Mori,H.,Nozawa,Y.: "Molecular approach to the toxic action of quinone mycotoxins:Chemical structure and biochemistry.In Current Topics of Medical Mycology 4,ed by M.Borgers et al." SpringerーVerlag,New York, (1991)

1990 Fiscal Year Annual Research Report

人及びラットの肝細胞癌,肝肉胆管癌の前駆病変細胞核DNA ploidyに関する研究

Principal Investigator

森 秀樹 岐阜大学, 医学部, 教授 (70021433)

Research Products

[Publications] Mori,H.,Yishimi,N.,Iwata,H.,Mori,Y.,Hara,A.,Tanaka,T.et al.: "Carcinogenicity of naturally occurring 1ーhydroxyanthraquinone in rats:induction of large bowel,liver and stomach neoplasms" Carcinogenesis. 11. 799-802 (1990)

[Publications] Mori,H.,Iwata,H.,Morishita,Y.,Mori,Y.,Ohno,T.,Tanaka,T.,Sasaki,S.: "Synergistic effect of radiation on Nー2ーfluorenylacetーamideーinduced hepatocarcinogenesis in male ACI/N rats" Japanese Journal of Cancer Research. 81. 975-978 (1990)

[Publications] Hara,A.,Yamada,H.,Sakai,N.,Hirayama,H.,Tanaka,T.,Mori,H.: "Immunohistochemical demonstration of the placental form of glutathione Sーtransferase,a detoxifying enzyme in human gliomas" Cancer. 66. 2563-2568 (1990)

[Publications] Tanaka,T.,Kojima,T.,Okumura,A.,Yoshimi,N.,Mori,H.: "Alterations of the nucleolar organizer regions during 4ーnitroquinoline 1ーoxideーinduced tongue carcinogenesis in rats" Carcinogenesis. 12. 329-333 (1991)

[Publications] Mori,Y.,Yoshimi,N.,Iwata,H.,Tanaka,T.,Mori,H.: "The synergistic effect of 1ーhydroxyanthraquinone on methylazoxymethanol acetateーinduced carcinogenesis in rats" Carcinogenesis. 12. (1991)

[Publications] Mori,H.,Sugie,S.,Okumura,A.,Hara,A.,Kinouchi,T.,Kataoka,K.,Ohnishi,Y.: "Genotoxicity of pyrene oxide and 1ーnitropyrene oxides in hepatocyte primary culture/DNA repair test" Mutation Research. (1991)

[Publications] Mori,H.: "Genotoxicity of naturallyーoccurring metabolites:structural analogs of aflatoxin and related chemicals.In Handbook of Applied Mycology,Vol.5 ed by D.Bhatnagar et al." Marcel Dekker Inc.,New York, (1991)

[Publications] Kawai,K.,Hisada,K.,Mori,H.,Nozawa,Y.: "Molecular approach to the toxic action of quinone mycotoxins:Chemical structure and biochemistry.In Current Topics of Medical Mycology 4,ed by M.Borgers et al." SpringerーVerlag,New York, (1991)

森秀樹岐阜大学, 医学部, 教授 (70021433)