1990 Fiscal Year Final Research Report Summary
Study on cell-nuclear DNA ploidy of precancerous population for hepatocellular carcinoma and cholangiocarcinoma of humans and rats
| Project/Area Number |
63570158
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Experimental pathology
|
|---|
| Research Institution | Gifu University |
|---|
Principal Investigator |
MORI Hideki Gifu Univ. Sch Med. Pathology Professor, 医学部, 教授 (70021433)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SUGIE Shigeyuki Gifu Univ. Sch Med. Pathology Research Associate, 医学部附属病院, 助手 (60187648)
TANAKA Takuji Gifu Univ. Sch Med. Pathology Instructor, 医学部, 講師 (40126743)
|
|---|
| Project Period (FY) |
1988 – 1990
|
| Keywords | Cell nuclear DNA ploidy / Altered hepatocellular focus / Microspectrophometer / Solt-Farber's model / N-2-Fluorenylacet-amide / Rat |
|---|
| Research Abstract |
Analysis of cell nuclear DNA ploidy patterns of precancerous hepatocellular population derived from different induction models was done using Feulgen-reacted spectrophotometer on sliced liver tissues. Precancerous hepatocellular population including altered hepatocellular foci were obtained in two different models I
1) SoltーFarber's model : single i. p. exposure of diethylnitrosamine 200 mg/kg, 2/3 hepatectomy and short term dietary exposure of N-2-fluorenylacetamide 2) continous dietary exposure of N-2-fluorenylacetamide (0.02%)]. The animals (male ACI/Nrats were sacrificed sequentially starting 4 weeks after the start of the experiment. Measurement of DNA content on the spectrophotometer (Olympus MMSP) was done using 545 nm on interphase and lymphocytes as diploid control. In the 1st and 2nd model, eosinophilic altered focus was the predominant type. DNA Ploidy pattern of this type of altered focus induced in the 1st model showed diploidization along the passage of time after the carcinogen exposure. Meanwhile, the ploidy pattern of the focus appeared in the model using continuous exposure of N-2-fluorenylacetamide did not show this tendency. The result suggests that altered hepatocelluar foci having similar phenotypic character could have different nuclear DNA ploidy pattern and the ploidy pattern of these precancerous population could depend on the induction methods including type of carcinogens and time and others. Hyperbasophilic focci infrequently appeared in both models. This type of focus had aneuploid pattern of DNA ploidy. Since many cells of hepatocellular carcinoma exhibit aneuploid pattern on DNA histogram. The results again indicate that this type of focus could be a direct precursor lesion for the hepatocellular malignancy.
|
Research Products
(16 results)
-
[Publications] Mori,H.,Yoshimi,N.,Iwata,H.,Mori,Y.,Hara,A.,Tanaka,T.,Kawai,K.: "Carcinogenicity of naturally occurring 1ーhydroxyanthraquinone in rats: induction of large bowel,liver and stomach neoplasms" Carcinogenesis. 11. 799-802 (1990)
Description
「研究成果報告書概要(和文)」より
-
[Publications] Mori,H.,Iwata,H.,Morishita,Y.,Mori,Y.,Ohno,T.,Tanaka,T.,Sasaki,S.: "Synergistic effect of radiation on Nー2ーfluorenylacetamideーinduced hepatocarcinogenesis in male ACI/N rats" Japanese Journal of Cancer Research. 81. 975-978 (1990)
Description
「研究成果報告書概要(和文)」より
-
[Publications] Hara,A.,Yamada,H.,Sakai,N.,Hirayama,H.,Tanaka,T.,Mori,H.: "Immunohistochemical demonstration of the plecental form of glutathione Sーtransferase,a detoxifying enzyme in human gliomas" Cancer. 66. 2563-2568 (1990)
Description
「研究成果報告書概要(和文)」より
-
-
[Publications] Mori,H.,Sugie,S.,Okumura,A.,Hara.A.,Kinouchi,T.,Kataoka,K.,Ohnishi,Y.: "Genotoxicity of pyrene oxide and 1ーnitropyrene oxides in hepatocyte primary culture/DNA repair test" Mutation Research. (1991)
Description
「研究成果報告書概要(和文)」より
-
-
-
[Publications] Kawai,K.,Hisada,K.,Mori,H.,Nozawa,Y.: "Molecular approach to the toxic action of quinone mycotoxins: chemical structure and biochemistry.In Current Topics of Medical Mycology 4,ed by M.Borgers et al." SpringerーVerlag,New York, (1991)
Description
「研究成果報告書概要(和文)」より
-
[Publications] Mori, H., Yoshimi, N., Iwata, H., Mori, Y., Hara, A., Tanaka, T., Kawai, K.: "Carcinogenicity of naturally occurring 1-hydro-xyanthraquinone in rats : induction of large bowel, liver and stomach neoplasms" Carcinogenesis. 11. 799-802 (1990)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Mori, H., Iwata, H., Morishita, Y., Mori, Y., Ohno, T., Sasaki, S.: "Synergistic effect of radiation on N-2-fluorenylacetamide-induced hepatocarcinogenesis in male ACI/N rats" Japanese Journal of Cancer Research. 81. 975-978 (1990)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Hara, A., Yamada, H., Sakai, N., Hirayama, H., Tanaka, T., Mori, H.: "Immunohistochemical demonstration of the placental form of glutathione S-transferase, a detoxifying enzyme in human gliomas" Cancer. 66. 2563-2568 (1990)
Description
「研究成果報告書概要(欧文)」より
-
-
-
[Publications] Mori, H., Sugie, S., Okumura, A., Hara, A., Kinouchi, A., Kataoka, K., Ohnishi, Y.: "Genotoxicity of pyrene oxide and 1-nitropyrene oxides in hepatocyte primary culture/DNA repair test" Mutation Research. (1991)
Description
「研究成果報告書概要(欧文)」より
-
-
