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1989 Fiscal Year Final Research Report Summary

Development of a dynamic analysis method for phagocytic activity utilizing flow cytometry

Research Project

Project/Area Number 63570170
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionKanazawa Medical University

Principal Investigator

FUJIKAWA Kohzaburo  Kanazawa Medical University, Research Institute of Medical Science, Assistant Professor, 総合医学研究所, 助教授 (20139786)

Project Period (FY) 1988 – 1989
KeywordsFlow cytometry / Phagocytosis / Microspheres
Research Abstract

A new method to analyze the phagoctic activity utilizing flow cytometry (FCM) was developed. We observed the changes in the fraction of cell phagocytizing microspheres and concluded that the phagocytosis of microspheres in V79 cultured cell line could be described with an experimental equation with two parameters. The results were shown in the following twelve sections. 1) Representative examples of FCM measurements of phagocytosis were shown. 2) Results form FCM and microscopic measurements were compared and the reliability of FCM measurements was confirmed. 3) How to distinguish phagocytized microspheres from attached those was explained. 4) Microsphere size suitable to the FCM measurements was examined. 5) Equilibrium of microspheres surrounding cells was examined. 6) Increase in coefficient of variation of peaks in fluorescence histograms was explained by the localization of microspheres in cells. 7) Phagocytic activity was compared between macrophages and V79 cells. 8) An experimental equation describing pliagocytosis was demonstrated. 9) Effects of microsphere concentration on the phagocytosis parameters k and n was examined. 10) A computer program to analyze the phagocytic activity was shown. 11) Cell cycle dependency of phagocytic activity was discussed. 12) Effects of hydroxyurea on the phagoeytosis were discussed.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Kohzaburo Fujikawa-Yamamoto: "Synergistic Effects of Hydroxyurea and Thimidine on the Growth Inhibition of V79 Cells" Cell Struct.Funct.14. 399-405 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kohzaburo Fujikawa-Yamamoto: "Effects of Hydroxyurea on the Phagocytosis in V79 Cells" Cell Struct.Funct.14. 485-493 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 藤川孝三郎: "培養細胞の貪食活性のFCM解析" フロ-サイトメトリ-. 9. 43-46 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kohzaburo Fujikawa-Yamamoto: "Synergistic Effects of Hydroxyurea and Thymidine on the Growth Inhibition of V79 Cells" Cell Struct. Funct.14. 399-405 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kohzaburo Fujikawa-Yamamoto: "Effects of Hydroxyurea on the Phagocytosis in V79 Cells" Cell Struct. Funct.14. 485-493 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kohzaburo Fujikawa: "FCM Analysis of Phagocytic Activity in Cultured Cells" Flow Cytometry. 9. 43-46 (1989)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-26  

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