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1990 Fiscal Year Final Research Report Summary

Studies on the Growth Hormone-Like Factor Produced by Plerocercoids of Spirometra erinacei.

Research Project

Project/Area Number 63570175
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 寄生虫学(含医用動物学)
Research InstitutionEhime University

Principal Investigator

HIRAI Kazumitsu  Ehime University School of Medicine, Parasitology ; Associate Professor, 医学部, 助教授 (20093940)

Co-Investigator(Kenkyū-buntansha) TSUBOI Takafumi  Ehime University School of Medicine ; Parasitology ; Research Associate., 医学部, 助手 (00188616)
Project Period (FY) 1988 – 1990
KeywordsGrowth hormone-like substance / Spirometra erinacei / Plerocercoid / Growth factor / Hepatocyte growth factor / Growth hormone receptor
Research Abstract

In order to examine the physiological property and partial amino acid sequence of the Growth Hormone (GH) -like factor produced by plerocercoids of Spirometra erinacei, we tried to purify this factor detected bt radio-receptor assay for GH using the GH receptor-coupled affinity chromatography. The GH-like factor were purified.
1) One of GH-like factor was purified by two steps consisted of the affinity chromatography and gel filtration.
2) The specific activity in this GH-like factor was enhanced to 195 times compared with the specific activity in the crude worm extract.
3) This factor exhibited the high affinity for the GH receptor and its molecular weight was estimated to be approximately 16 KD by SDS-polyacryl amide gel electrophoresis.
4) This factor stimulated the prolifelation of the mouse hepatocyte in vitro, and then suppressed the serum level of glucose when this factor was injected into the femoral vein of a golden hamster compared with the control.
5) This 16 KD protein obviously cross-reacted with anti-human GH monoclonal antibody on the immunoblot method.
6) The 27 KD protein, which had low affinity for the GH receptor, was also purified from the worm extract and cross-reacted with anti-human GH-monoclonal antibody. Moreover, this stimulated the proliferation of the mouse hepatocyte in vitro.
7) There was the homology of 67% between amino acid sequences of cathepsin L and the fragment of the 27 KD protein cleavaged by lysil endo peptidase.

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 坪井 敬文他: "マンソン裂頭条虫擬充尾虫由来の肝細胞増殖因子" 寄生虫学雑誌. 39. 84- (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 平井 和光他: "マンソン裂頭条虫擬充尾虫が産生する肝細胞増殖因子" 愛媛医学. 9. 525-532 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Phares,C.K.et al.: "Evolutionary imprications of a human growth hormonelike factor in the tapeworm genus Spirometra." Advances in invertebrate reproduction 5.5. 169-174 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsuboi,T.et al.: "Decrease of liver glycogen content in golden hamsters infected with plerocercoids of Spirometra erinacei." Parasitology Research. (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirai,K.et al.: "Effect of infection with Spirometra erinacei plerocercoids on thyroid hormone in mice." Parasitology Reseach. 74. 267-270 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirai,K.et al.: "Hepatocyte proliferating activity of a growth hormonelike factor from plerocercoids of the tapeworm,Spirometra erinacei." Abstracts of 5th International Congress of Invertebrate Reproduction.148- (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 平井 和光: "マンソン裂頭条虫擬充尾虫由来の成長促進因子ーSparganum Growth Factorの生理作用." 医学のあゆみ. 146. 836- (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 平井 和光他: "マンソン裂頭条虫擬充尾虫由来の成長ホルモン様物貭の精製とその特性" 寄生虫学雑誌. 38. 87- (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 坪井 敬文他: "マンソン裂頭条虫擬充尾虫によるマウス肝細胞増殖促進作用" 寄生虫学雑誌. 38. 87- (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 平井 和光他: "マンソン裂頭条虫擬充尾虫が産生する成長ホルモン様物貭の特性" 寄生虫学雑誌. 39. 84- (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirai, K. et al.: "Effect of infection with Spirometra erinacei plerocercoids on thyroid hormone in mice." Parasitology Research. 74(3). 267-270 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirai, K. et al.: "Growth factor induced by plerocercoids of Spirometra erinacei-Physiological activity of Sparganum Growth Factor." Igaku no Ayumi. 146(2). 836 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirai, K. et al.: "Hepatocyte growth factor produced by the plerocercoid of Spirometra erinacei." Ehime Medical Journal. 9(3). (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Phares, C. K. et al.: "Evolutionary implication of a human growth hormone-like factor in the tapeworm genus Spirometra." Advances in invertebrate reproduction. 5. 169-174 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuboi, T. et al.: "Decrease of liver glycogen content in golden hamsters infected with plerocercoids of Spirometra erinacei." Parasitology Research. (1990)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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