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1989 Fiscal Year Final Research Report Summary

Correlation between infection mechanisms and virulence plasmid of Salmonella choleraesuis

Research Project

Project/Area Number 63570205
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 細菌学
Research InstitutionThe Kitasato Institute

Principal Investigator

DANBARA Hirofumi  The Kitasato Institute, Dep. Bacteriol. 2nd Lab., Chief of Laboratory, 細菌部第2室, 室長 (40114558)

Co-Investigator(Kenkyū-buntansha) MATSUI Hidenori  The Kitasato Institute, Dep. Bacteriol. 2nd Lab., Senior Researcher, 細菌部第2室, 研究員 (30219373)
KAWAHARA Kazuyoshi  The Kitasato Institute, Dep. Bacteriol. 2nd Lab., Subchief of Laboratory, 細菌部第2室, 室長補佐 (20195126)
MORIGUCHI Ryozo  The Kitasato Institute, Dep. Pathol. 1st Lab., Chief of Laboratory, 病理部第1室, 室長 (30101299)
Project Period (FY) 1988 – 1989
KeywordsSalmonella choleraesuis / Salmonella / Virulence plasmid / Bacteremia
Research Abstract

1. Correlation between virulence plasmid of Salmonella choleraesuis, pKDSC50, and its mouse virulence: S. choleraesuis strains harboring virulence plasmid, pKDSC50 (parent and re-introduced strains), and cured strains were inoculated intraperitoneally into ICR mice, and the mice were examined pathologically for 14 days. Parent and re-introduced strains caused bacteremia in mice, and inoculated bacteria were recovered from spleen and liver. Obvious pathological changes were observed at individual tissues of peritoneal fluid, mesenterium, and visceral organs, and those changes persisted to day 14. In contrast, mice infected with plasmid-cured strain showed no bacteremia, and had only mild and transient pathological changes at peritoneal fluid, mesenterium, and viscera.
2. Difference of cell surface components between pKDSC50-harbouring and -cured strains of S. choleraesuis : Lipopolysaccharide, outer membrane proteins, and phospholipids of parent, re-introduced, and cured strains were che … More mically and biochemically compared. There was no significant difference between them, indicating that pKDSC50 did not mediate the detectable change of bacterial cell surface.
3. Mapping of the region responsible for the ability to cause mouse bacteremia by transposoninsertional inactivation: Mouse bacteremia test was established in the base of the results of pathological study, and used for screening 100 mutant strains constructed by transposon Tnl-insertional inactivation. Plasmid DNA of mouse bacteremia test-negative strains were introduced to cured strain by transformation, and the transformants were again tested for mouse bacteremia ability. Nine strains were negative for the second test, and Tnl on their plasmids were localized in 6.2 kb region of pKDSC50. This region was designated mba region.
4. Genetical study on mba region of pKDSC50: DNA fragments of mba region were cloned in E. coli, and proteins encoded in this region were identified by E. coli minicell system, or by lac-promoter system with induction of isopropylthiogalactoside. Four proteins with apparent molecular weights of 29k, 70k, 32k, and 32k were detected, and open reading frames corresponding to these four proteins were found in mba region by nucleotide sequential analysis. Other research groups has recently reported similar proteins encoded in virulence plasmid of S. typhimurium, suggesting that the proteins we found were common among virulence plasmid-harboring Salmonella, and playing important role in pathogenesis of Salmonella. Less

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] K.Kawahara,Y.Haraguchi,M.Tsuchimoto,N.Terakado,H.Danbara: "Evidence of correlation between 50-kilobase plasmid of Salmonella choleraesuis and its virulence" Microbial Pathogenesis. 4. 155-163 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Kawahara,T.Hamaoka,S.Suzuki,M.Nakamura,S.Y.Murayama,T.Arai,N.Terakado,H.Danbara: "Lipopolysaccharide alteration mediated by the virulence plasmid of Salmonella" Microbial Pathogenesis. 7. 195-202 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Kawahara,M.Tsuchimoto,K.Sudo,N.Terakado,H.Danbara: "Identification and mapping of mba regions of the Salmonella choleraesuis virulence plasmid pKDSC50 responsible for mouse bacteremia" Microbial Pathogenesis. 8. (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Matsui,K.Kawahara,N.Terakado,H.Danbara: "Nucleotide sequence of a gene encoding a 29 KDa polypeptide in mba region of the virulence plasmid,pKDSC50,of Salmonella choleraesuis" Nucleic Acids Research. 18. (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Matsui,K.Kawahara,N.Terakado,H.Danbara: "Nucleotide sequence of genes encoding 32 KDa and 70 KDa polypeptides in mba region of the virulence plasmid,pKDSC50,of Salmonella choleraesuis" Nucleic Acids Research.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] R.Moriguchi,K.Kawahara,Y.Haraguchi,Y.Nakase,H.Danbara: "Pathological study on virulence of Salmonella choleraesuis in mice associated with 50-kilobase plasmid" British J.Experimental Pathology.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Danbara,K.Kawahara,M.Tsuchimoto,R.Moriguchi,N.Terakado: "“Characterization of 50-kilobase plasmid of S.choleraesuis strain AH1-S44"In;Cellular and Molecular Mode of Action of Selected Microbial Toxins in Foods and Feeds" Plenum Press,New York/London, (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K. Kawahara, Y. Haraguchi, M. Tsuchimoto, N. Terakado, H. Danbara.: "Evidence of correlation between 50-kilobase plasmid of Salmonella choleraesuis and its virulence." Microbial Pathogenesis 4, 155-163, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Kawahara, T. Hamaoka, S. Suzuki, M. Nakamura, S.Y. Murayama, T. Arai, N. Terakado, H. Danbara.: "Lipopolysaccharide alteration mediated by the virulence plasmid of Salmonella." Microbial Pathogenesis 7, 195-202, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Kawahara, M. Tsuchimoto, K. Sudo, N. Terakado, H. Danbara.: "Identification and mapping of mba regions of the Salmonella choleraesuis virulence plasmid pKDSC50 responsible for mouse bacteremia." Microbial Pathogenesis 8, 1990.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Matsui, K. Kawahara, N. Terakado, H. Danbara.: "Nucleotide sequence of genes encoding 32 kDa and 70 kDa polypeptides in mba region of the virulence plasmid, pKDSC50, of Salmonella choleraesuis." Nucleic Acids Research 18, 1990.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Matsui, K. Kawahara, N. Terakado, H. Danbara.: "Nucleotide sequence of genes encoding 32 kDa and 70 kDa polypeptides in mba region of the virulence plasmid, pKDSC50, of Salmonella choleraesuis." Nucleic Acids Research.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] R. Moriguchi, K. Kawahara, Y. Haraguchi, Y. Nakase, H. Danbara.: "Pathological study on virulence of Salmonella choleraesuis in mice associated with 50-kilobase plasmid." British J. Experimental Pathology.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Danbara, K. Kawahara, M. Tsuchimoto, R. Moriguchi, N. Terakado.: "Characterization of 50-kilobase plasmid of S. choleraesuis strain AH1-S44. In: Cellular and Molecular Mode of Action of Selected Microbial Toxins in Foods and Feeds." Plenum Press, New York/London, 1990.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-26  

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