1989 Fiscal Year Final Research Report Summary

The Distribution of the Blood Group A- and B-active Glycolipids in the Human Erythrocyte Membrane

Research Project

Project/Area Number	63570276
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Legal medicine
Research Institution	University of the Ryukyus
Principal Investigator	UCHIMA Eikoh University of the Ryukyus, School of Medicine, Department of Legal Medicine, Instructor, 医学部, 助手 (90093377)
Project Period (FY)	1988 – 1989
Keywords	Blood groups / Glycolipids / Immunostaining / Monoclonal antibody / Chromatoscanner
Research Abstract	A quantitative study on the distribution of structural variations of the A- and/or B-active glycolipids in human blood group A, B and AB erythrocyte membranes was performed. In order to detect the glycolipids with a blood group specificity, immunostaining on a thin-layer chromatography plate, using a monoclonal antibody, was applied, and the relative antigenic ratio of each glycolipid was determined by the use of a chromatoscanner. The results indicated that the blood group active glycolipids with shorter and simpler carbohydrate chains were found to have a greater distribution on the erythrocyte membranes than those with longer and more complex carbohydrate chains. Further, it was found that the antigenic ratios of four A-active glycolipids from the blood A and AB erythrocyte membranes were similar, and that this also was observed with regard to the six B-active glycolipids from the blood group B and AB erythrocytes.

(2 results)