1989 Fiscal Year Final Research Report Summary
Significance of Fibrin/Fibrinogen Related Antigen in Glomeruli in Children with IgA Nephropathy
| Project/Area Number |
63570453
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Department of Pediatrics, Nara Medical College |
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Principal Investigator |
TAIRA Koji Department of Pediatrics, Nara Medical College, Assistant, 医学部, 助手 (10201592)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUI Hiromu Department of Pediatrics, Nara Medical College, Professor, 医学部, 教授 (20075104)
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| Project Period (FY) |
1989
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| Keywords | IgA nephropathy / fibrin / fibrinogen related antigen / FDP / D-dimer / monochloroacetic acid(MCA) / protein-A gold |
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| Research Abstract |
The localization of intraglomerular deposits of fibrin(fb) /fibrinogen(fg)-related antigen(FRA) in children with IgA nephropathy was determined by an immunohistopathologic method using an anti-fg antibody capable of detecting FRA, anti-D-dimer antibody capable of detecting crosslinked Fb, and by a method using the effect of monochloroacetic acid(MCA) treatment on kidney sections. In addition, urinary FDP and thrombin-anti- thrombin III complex(TAT) were examined. MCA-insoluble FRA, suggesting cross-linked fibrin, was observed within the capillary walls, the mesangium and/or the sclerotic area in children with sever proliferative lesions, such as segmental sclerosis, capsular adhesions or crescent formation. on the other hand, MCA-soluble FRA was mainly observed in mesangial area and suggested to be associated with immune-complex deposits in the glomeruli. Mesangial deposits of FRA were confined to electron-dense deposits(EDD). MCA-soluble FRA did not seem to be closely related to glomerular damage. In children with sever proliferative lesions, high level of FDP, and D-dimer and/or TAT were detected in the urine.
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