1989 Fiscal Year Final Research Report Summary
THE ANALYSIS OF THE IMMUNOLOGIC ROLE OF EPIDERMAL LANGERHANS CELLS IN THE INDUCTION OF CYTOTOXIC T LYMPHOCYTE (CTL)
| Project/Area Number |
63570480
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Showa University |
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Principal Investigator |
IIJIMA Masafumi Showa University, Faculty of Medicine, Department of Dermatology : Associated Professor., 医学部・皮膚科, 助教授 (20010449)
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| Co-Investigator(Kenkyū-buntansha) |
OGURA Miyoko Showa University, Faculty of Medicine, Department of Dermatology : Assistant Pro, 医学部・皮膚科, 助手
DAITA Yumi Showa University, Faculty of Medicine, Department of Dermatology : Assistant Pro, 医学部・皮膚科, 助手
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| Project Period (FY) |
1988 – 1989
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| Keywords | Langerhans cell (LC) / Ia+APC function / CTL induction in vitro / Tissue-culture / Murine tail LC / Functional maturation of LC by tissue-culture / PT-GVHD / Immunohistological differential diagnosis for PT-GVHD |
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| Research Abstract |
Tissue-cultured, not but freshly-prepared murine tail epidermal LANGERHANS CELLS(LC) can induce cytotoxic T lymphocyte (CTL) in vitro.
We had already reported that freshly-prepared murine tail LC could not induce CTL in vitro, whereas LC of ear could do. (IIJIMA M.: Jpn J Dermatol,96:1143-1150,1986) In this study, we investigated whether murine tail LC possess Ia-antigen-positive, antigen presenting cell (Ia+APC) function or not even after tissue-culture in vitro. When mouse ear LC were tissue-cultured before mixed lymphocyte-epidermal cell culture to induce CTL, they could exhibit extremely enhanced APC function accompanied with increased expression of Ia antigens on their cell surface. Tissue-cultured murine tail LC were able to induce CTL, which freshly-prepared tail LC had never produced. Thus, it was proven for the first time that tissue-cultured, not but freshly-prepared murine tail LC could act as Ia+APC in the induction of CTL in vitro.
Ear LC, which were tissue-cultured for more than 48 hrs, induced extremely enhanced levels of CTL over those produced by freshly-prepared ear LC. Furthermore LC that were tissue-cultured with dermal components exhibited much more enhanced Ia+APC function than LC which were tissue-cultured in single cell suspension with only epidermal cells. We also found that strongly enhanced Ia+APC function of tissue-cultured LC was completely abolished by anti-Ia antibody plus complement treatment and irradiation of UVB at the dose of 600 J/m^2.
We also investigated the role of LC in the immunohistological rapid diagnosis of so-called "post- operative erythroderma", which was caused by mortal post-transfusion graft-versus-host disease (PT- GVHD) occurred in the Japanese patients without immunodeficiency. We stressed that immunohistological presence of intraepidermal LC was most important in order to differentiate from PT-GVHD in the post- transfusion patients with a high fever and generalized erythematous skin rash.
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