1989 Fiscal Year Final Research Report Summary
Biosynthesis of Eumelanin in Melanoma and Biochemical Markers of Melanoma
| Project/Area Number |
63570484
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Fujita Health University |
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Principal Investigator |
ITO Shosuke Fujita Health Univ, Sch of Hygiene Professor, 衛生学部, 教授 (70121431)
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| Co-Investigator(Kenkyū-buntansha) |
WAKAMATSU Kazumasa Fujita Health Univ, Sch of Hygiene Asst Prof, 衛生学部, 講師 (80131259)
INOUE Shigeki Fujita Health Univ, Sch of Hygiene Asst Prof, 衛生学部, 講師 (30084601)
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| Project Period (FY) |
1988 – 1989
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| Keywords | Melanin / Malignant melanoma / Dopa / HPLC |
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| Research Abstract |
1. Simplified method for preparing enmelanin-related metabolites Dopachrome solution, firepared by chemical oxidation of dopa, was kept under different pH to yield 5,6DHI and 5,6DHI2C. Treatment with diazomethane afforded their O-methyl derivatives. This enabled us to obtain standard compounds necessary for the following research.
2. Importance of enmelanin-related metabolites as biochemical markers of melanoma (1) Excretion of melanin-related metabolites was examined in mice bearing B16 melanoma. In early stage of tumor progression, the increase of 5H6MI2C was found to be more pronounced than of 5-S-CD, a commonly used marker. (2) Correlation among the contents of melanin-related metabolites in tumor, serum, and urine was examined in mice bearing B16 melanoma. Good correlation was found between serum indoles (5H6MI2C + 6H5MI2C) or 5-S-CD and tumor weight (r = 0.92, 0.89, respectively). Urinary metabolites did not show a significant correlation. These results suggest that melanin-related metabolites in serum may better reflect progression of melanoma than those in urine. (3) High levels of glucronide and sulfate conjugates of indoles were found in urine of a melanoma patient. Only sulfate conjugates were detected in urine of melanoma-bearing mice.
Serum and urine samples from healthy subjects and melanoma patients will be analyzed for indoles and 5-S-CD to compare their usefulness in early diagnosis and prognosis of melanoma.
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